BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0040.p (466 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 901 0.0 gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 95 1e-20 gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 80 4e-16 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 901 bits (2329), Expect = 0.0, Method: Compositional matrix adjust. Identities = 439/466 (94%), Positives = 455/466 (97%) Query: 1 MLKTLYDKLWESHVVHAEQDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNAANLVV 60 MLKTLYDKLWESHVVH E DGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRN+ANL+V Sbjct: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 Query: 61 ADHNVPTTNRANGIADPISRLQVETLDANAKEYGLTYFGMNDKRQGIVHVIGPEQGATLP 120 ADHNVPTT+RA+GIADPISRLQVETLD NAKEY LTYFGMNDKRQGIVHVIGPEQGATLP Sbjct: 61 ADHNVPTTDRAHGIADPISRLQVETLDGNAKEYSLTYFGMNDKRQGIVHVIGPEQGATLP 120 Query: 121 GMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVLATQTLLAKKSKSMLVQVDGALPNGVTA 180 GMTVVCGDSHTSTHGAFA LAHGIGTSEVEHVLATQTLLA+KSK+MLVQVDGALP GVTA Sbjct: 121 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA 180 Query: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTMRALTMEGRMTVCNMAIEAGARAGMIAVDDTTI 240 KDIVLAVIGKIGTAGGTGYAIEFAGST+R+L+MEGRMTVCNMAIEAGARAGM+A DDTT+ Sbjct: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL 240 Query: 241 NYLKGRPFSPAGPHWERAVSYWRTLHSDPGAKFDMVVTLNATEIKPQVTWGTSPEMVIAI 300 YLKGRPFSP+GPHWERAVSYWR+LHSDPGAKFDMVVTLNA EIKPQVTWGTSPEMV+A+ Sbjct: 241 EYLKGRPFSPSGPHWERAVSYWRSLHSDPGAKFDMVVTLNAAEIKPQVTWGTSPEMVVAV 300 Query: 301 DSRVPDPDKEKDATKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAA 360 D+RVPDPDKEKD TKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAA Sbjct: 301 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK 360 Query: 361 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE 420 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE Sbjct: 361 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE 420 Query: 421 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL 466 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL Sbjct: 421 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL 466 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 95.1 bits (235), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 114/396 (28%), Positives = 162/396 (40%), Gaps = 36/396 (9%) Query: 98 FGMNDKRQGIVHVIGPEQ---------GATLPGMTVVCGDSHTSTHGAFACLAHGIGTSE 148 FG+ GIVH + E G P T+V DSHT+ + G+G E Sbjct: 171 FGVVPPGFGIVHQVNLEYLARGVHNKTGVYYPD-TLVGTDSHTTMINGIGVVGWGVGGIE 229 Query: 149 VEHVLATQTLLAKKSKSMLVQVDGALPNGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTM 208 E + Q + + V + GAL GVTA D+VL + + G +EF G Sbjct: 230 AEAGMLGQPVYFLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGT 289 Query: 209 RALTMEGRMTVCNMAIEAGARAGMIAVDDTTINYLKGRPFSPAGPHWERAVSYWRTLHSD 268 +L++ R T+ NMA E GA G VDD TI Y KG + A A + L+ Sbjct: 290 ESLSLTDRATIANMAPEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGV 349 Query: 269 PGA---KFDMVVTLNATEIKPQVTWGTSPEMVIAI-DSRVPDPDKEKDATKRDGM-EKAL 323 P A + V+L+ + P + P+ I I + + D T +G +KA Sbjct: 350 PKAGQIDYTQEVSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAE 409 Query: 324 AYMALKPNTPIEDIRIDKVFIG---SCTNSRIEDLREAAAVVRGKFRASNVKLA-----M 375 A N D+ V I SCTN+ + AA ++ K + +K+A Sbjct: 410 DLGASYKNADGVDLHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTS 469 Query: 376 VVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPGERCAS-------- 427 + PGS +V + E GL GF GC+ C+ NA L P A Sbjct: 470 LAPGSRVVTKYLEAAGLLPYLEKLGFGVTAYGCTTCIG-NAGDLTPAMNEAIVKNDVVAA 528 Query: 428 ---TSNRNFEGRQGAGGRTH-LVSPAMAAAAGIAGH 459 + NRNFE R R + L SP + A IAG+ Sbjct: 529 AVLSGNRNFEARIHPNIRANFLASPPLVVAYAIAGN 564 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 80.1 bits (196), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 111/403 (27%), Positives = 166/403 (41%), Gaps = 47/403 (11%) Query: 79 SRLQVETLDANAKEYGLTYFGMNDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFA 138 ++L E +D G+ + +K ++H + G P T+V DSHT A Sbjct: 155 TKLAFENVDVIPPGNGIMHQINLEKMSPVIHA---KDGVAFPD-TLVGTDSHTPHVDALG 210 Query: 139 CLAHGIGTSEVEHVLATQTLLAKKSKSMLVQVDGALPNGVTAKDIVLAVIGKIGTAGGTG 198 +A G+G E E+V+ + + + V++ G G+TA DIVL++ + G Sbjct: 211 VIAIGVGGLEAENVMLGRASWMRLPDIIGVELTGRRQPGITATDIVLSLTEFLRKQKVVG 270 Query: 199 YAIEFAGSTMRALTMEGRMTVCNMAIEAGARAGMIAVDDTTINYLK--GRPFSPAGPHWE 256 +EF G +LT+ R T+ NMA E GA A M ++D TI+YLK GR + Sbjct: 271 AYLEFYGDGAASLTLGDRATISNMAPEYGATAAMFSIDQQTIDYLKLTGRE--------D 322 Query: 257 RAVSYWRTLHSDPGAKFDMVVTLNATEIKPQVTWGTSPEMVIAIDSRVPDPDKEKDATKR 316 V T + G D TL E + + + S V+ + +P K + Sbjct: 323 EQVKLVETYAKEAGLWSD---TLAKVEYERVLRFDLS--TVVRTLAGPSNPHKRLPVS-- 375 Query: 317 DGMEKALAYMALK-PNTP--IEDIRIDKVFIGSCTN-SRIEDLREAAAVVR-----GKFR 367 E A +A K N P + D + I SCTN S ++ A + R G R Sbjct: 376 ---ELAAQGIAGKVENEPGKMPDGAVIIAAITSCTNTSNPRNVIAAGLLARNANRLGLAR 432 Query: 368 ASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPG----- 422 VK ++ PGS V+ E GL GF C+ C M+ L+P Sbjct: 433 KPWVKSSL-APGSKAVELYLEEAGLTADLEKLGFGIVAFACTTCNGMSG-ALDPKIQQEI 490 Query: 423 ------ERCASTSNRNFEGR-QGAGGRTHLVSPAMAAAAGIAG 458 + NRNF+GR + L SP + A IAG Sbjct: 491 IDRDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAG 533 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,483,062 Number of extensions: 62330 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 3 Length of query: 466 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 377 Effective length of database: 1,198,455 Effective search space: 451817535 Effective search space used: 451817535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)