BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0027.p (178 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 163 8e-42 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 145 3e-36 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 130 8e-32 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 89 3e-19 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 163 bits (412), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 82/170 (48%), Positives = 114/170 (67%), Gaps = 2/170 (1%) Query: 1 MNVIGNMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVAKATQAMMDVPLAKERMIGDWY 60 MN+IGNM IG+RL LGFA+IL S++IA IG+WRL VA+AT+ MM PL ER++ DWY Sbjct: 1 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY 60 Query: 61 RHTYSGIRRATAVAKSSDPSLSAFFKPETDEATRISTELQKKILELLVSDEEKQLYEKIM 120 + +GIRR A+AKSSDP+L +F E +++ S E QK++ LL + EEK L++KI Sbjct: 61 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG 120 Query: 121 SLRKVYLAKRDVIYKAKGEPNPDPAMIADALEKDYLPAAKNYSTEVENLL 170 LRK+YL+ RD I KAK N + AM L++ ++P A Y + L+ Sbjct: 121 ELRKLYLSSRDEINKAKAAGNTEEAM--RILDQVFVPNATAYQDTMRKLV 168 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 145 bits (365), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 78/162 (48%), Positives = 105/162 (64%), Gaps = 2/162 (1%) Query: 1 MNVIGNMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVAKATQAMMDVPLAKERMIGDWY 60 M NMKIG+RL LGFA+ILA + ++ + +WRLQ +A AT MM PL KER++ DWY Sbjct: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 Query: 61 RHTYSGIRRATAVAKSSDPSLSAFFKPETDEATRISTELQKKILELLVSDEEKQLYEKIM 120 R ++ +RR TA+AKSSDPSL+AFF + A+R+STE QK I L+ SD+EK ++ K+ Sbjct: 61 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG 120 Query: 121 SLRKVYLAKRDVIYKAKGEPNPDPAMIADALEKDYLPAAKNY 162 +RK Y+ RD I KAK E + A A L + A K Y Sbjct: 121 EVRKDYIKYRDAISKAKAEGRTEEA--AQILAGPFDVAGKGY 160 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 130 bits (326), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 67/168 (39%), Positives = 100/168 (59%), Gaps = 2/168 (1%) Query: 3 VIGNMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVAKATQAMMDVPLAKERMIGDWYRH 62 +I +++IG RLG GFA++L S+++A +G+WRL++VA TQ MMDVPL ER++ W Sbjct: 1 MIKDIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTL 60 Query: 63 TYSGIRRATAVAKSSDPSLSAFFKPETDEATRISTELQKKILELLVSDEEKQLYEKIMSL 122 I+R T V KS DP L AF E +++ S + K + +LL S+EEK+++ I Sbjct: 61 LLIAIQRTTTVVKSHDPELEAFLAKEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVA 120 Query: 123 RKVYLAKRDVIYKAKGEPNPDPAMIADALEKDYLPAAKNYSTEVENLL 170 RK +L RD IY AK D A + + +K Y+ AK+ + LL Sbjct: 121 RKQFLTIRDQIYAAK--KQGDAAQVEELYQKQYVGIAKSTQDAMRALL 166 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 88.6 bits (218), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 50/133 (37%), Positives = 75/133 (56%), Gaps = 2/133 (1%) Query: 6 NMKIGKRLGLGFAIILALSVLIAVIGVWRLQSVAKATQAMMDVPLAKERMIGDWYRHTYS 65 N+KIG RL +GF +L L ++ +GVWRL S+ AT M + L KER W+ Sbjct: 3 NLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKE 62 Query: 66 GIRRATAVAKSSDPSLSAFFKPETD-EATRISTELQKKILELLVSDEEKQLYEKIMSLRK 124 R AV KS D + A+F+ + D ++ RIS +LQK++ + EEK+L++++ LR Sbjct: 63 NGVRTFAVMKSDDDAFQAYFQKQIDAQSARIS-QLQKEVEAAITDAEEKRLFDEVGKLRA 121 Query: 125 VYLAKRDVIYKAK 137 Y A R I+ K Sbjct: 122 AYRATRQSIFDVK 134 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 426,513 Number of extensions: 13439 Number of successful extensions: 84 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 84 Number of HSP's successfully gapped: 5 Length of query: 178 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 99 Effective length of database: 1,245,805 Effective search space: 123334695 Effective search space used: 123334695 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)