BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0026.p (683 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 1025 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 400 e-112 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 384 e-107 gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 379 e-106 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 378 e-106 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 1025 bits (2649), Expect = 0.0, Method: Compositional matrix adjust. Identities = 524/678 (77%), Positives = 590/678 (87%), Gaps = 3/678 (0%) Query: 6 AVLASYTALDMTERINATQEYAGRS---WLLGGAVAMGIGIWSMHFVGMLAFRLPIDLGY 62 A+LASYTALDM ERIN T GR+ WL GGAVAMG+GIWSMHF+GMLAFRLPI LGY Sbjct: 18 AILASYTALDMAERINTTASQHGRAARWWLAGGAVAMGLGIWSMHFIGMLAFRLPIALGY 77 Query: 63 DPWITFLSLLIAIGVSGFALWIVSRPELPLRRLLNSALAMGVGIAAMHYTGMAALLMMPG 122 D IT LSL+IAI VSGFALWIV+RPELP RRLL SAL MG+GIA MHYTGMAAL M PG Sbjct: 78 DAGITALSLVIAIAVSGFALWIVTRPELPTRRLLGSALFMGIGIACMHYTGMAALRMQPG 137 Query: 123 IHYDPLLFAASVAIAIAASGAALWIAFRLRRNTPHVKLARGGAAVVMGVAIVGMHYTGMA 182 I YD LF ASVAIAIAAS AALWIAF LRRNTPHV+ ARGGA+VVMG+AI GMHYTGM Sbjct: 138 IAYDLFLFLASVAIAIAASAAALWIAFSLRRNTPHVRAARGGASVVMGIAIFGMHYTGMG 197 Query: 183 AANFPEGSFCLAVRDGVSPGWLAILIIVVTLAVLTIALLTSLLDARLESRTAKLARSLAE 242 AA+F S CL+ R+GV+PGWLA+LIIVVTLA LTIALLTS+LDARLESRTAKLARSLA Sbjct: 198 AASFSRDSICLSAREGVAPGWLAVLIIVVTLAALTIALLTSVLDARLESRTAKLARSLAA 257 Query: 243 ANEELTQLVLHDNLTKLPNRTLLDDRLNQAISKAAREQGHFALMFCDLDGFKAINDSLGH 302 ANEELTQ+VLHD LT+LPNRTLL+DRLNQAI+KAAREQGHFALMF DLDGFKAINDSLGH Sbjct: 258 ANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGH 317 Query: 303 HVGDLMLVEVAQRIRSVMRAQDTVARLGGDEFVMLVDLKEPDDAVTVADKLVKIINQSFN 362 HVGD +LVEVAQR+ S RA DTVARLGGDEFV+LVDL P+DA+ VA+KLV+ +NQ F Sbjct: 318 HVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPEDAMGVAEKLVEAVNQPFK 377 Query: 363 IEKHELRVSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRNGYHFFEASMNVNAHNQ 422 +++HELRVSAS+GIAIYPEDG++RHDLVINADAAMYHTKRSGRNGYHFFE SMN NA Q Sbjct: 378 VDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQQ 437 Query: 423 LQWLQDLRLALERKEFILHYQPKYNSPNGPVLGAEALLRWQHPVHGMVGPDEFIGLAERS 482 LQWLQDLR+ALER +F L YQPK++ P GPVLGAEALLRW+HPVHG+V PD+FIGLAERS Sbjct: 438 LQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAERS 497 Query: 483 GLIVPIGAWVLDEACRQMKVWYDIGHEDWKIAVNLSALQFAQTDLIDLVKDTLARHDLPA 542 GLI+PIGAWVLDEACRQM+ W+ +G+ DW ++VNLSALQFA L+++V+ TL RH LP Sbjct: 498 GLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLPP 557 Query: 543 RCLTLEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYSSLLYLKRLPANELKIDR 602 CLTLEVTESTAMHD E+SL+TL+QIADLGV+ISIDDFGTGYSSLLYLKRLPANELKIDR Sbjct: 558 PCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKIDR 617 Query: 603 GFVRDLSDGNDDAAIISAIVALGRTLNLRIVAEGVETTKQQNFLTSVGCDALQGYLMGRP 662 GFVR+L +DAAI+SAIVALGRTLNLRIVAEGVET +QQ+FLT+ GCDALQGYLMGRP Sbjct: 618 GFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGRP 677 Query: 663 MPPDKFIEATSNLMKNTA 680 M P+ F++ K+ A Sbjct: 678 MTPELFLQTMRERHKSAA 695 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 400 bits (1028), Expect = e-112, Method: Compositional matrix adjust. Identities = 209/433 (48%), Positives = 276/433 (63%), Gaps = 5/433 (1%) Query: 239 SLAEANEELTQLVLHDNLTKLPNRTLLDDRLNQAISKAAREQGHFALMFCDLDGFKAIND 298 S+ EL D LT L NR +L L QAI A R++ ++F DLD FK +ND Sbjct: 507 SMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVND 566 Query: 299 SLGHHVGDLMLVEVAQRIRSVMRAQDTVARLGGDEFVMLVDLKEPDDAVTVADKLVKIIN 358 +LGH GD +L VA R+R+ +R DTVARLGGDEF+M+ L E D+ L +I++ Sbjct: 567 TLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMI--LPEQDELTLDTRSLQRIMD 624 Query: 359 Q---SFNIEKHELRVSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRNGYHFFEASM 415 + H L ++ S+G+A YP DG+ L+ +AD AMY K++GRN + F+ +M Sbjct: 625 AVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAM 684 Query: 416 NVNAHNQLQWLQDLRLALERKEFILHYQPKYNSPNGPVLGAEALLRWQHPVHGMVGPDEF 475 N A +L+ DLR AL+ EF+LHYQPK + +G V+G+EAL+RWQHP GMV P F Sbjct: 685 NEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARF 744 Query: 476 IGLAERSGLIVPIGAWVLDEACRQMKVWYDIGHEDWKIAVNLSALQFAQTDLIDLVKDTL 535 IGLAE +GLI+PIGAWVL AC Q + W G+ D ++VNLS QF Q DL+ + D L Sbjct: 745 IGLAEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVL 804 Query: 536 ARHDLPARCLTLEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYSSLLYLKRLPA 595 LP L LE+TES M D E ++ L + LGV +SIDDFGTGYSSL YLKR P Sbjct: 805 EDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPI 864 Query: 596 NELKIDRGFVRDLSDGNDDAAIISAIVALGRTLNLRIVAEGVETTKQQNFLTSVGCDALQ 655 + LKIDR FVRD++ DDAAI ++I++L R L LR++AEGVET +Q ++L GCD +Q Sbjct: 865 DVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQ 924 Query: 656 GYLMGRPMPPDKF 668 GYL P+P DKF Sbjct: 925 GYLFSPPVPADKF 937 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 384 bits (987), Expect = e-107, Method: Compositional matrix adjust. Identities = 200/421 (47%), Positives = 269/421 (63%), Gaps = 5/421 (1%) Query: 246 ELTQLVLHDNLTKLPNRTLLDDRLNQAISKAAREQGHF-ALMFCDLDGFKAINDSLGHHV 304 E+ HD LT LPNR +L + L +A+ + AR++G A+ F DLD FK INDSLGH V Sbjct: 261 EMRHQATHDALTGLPNRNMLHEHLQRAL-RVARQKGALTAVAFVDLDQFKFINDSLGHQV 319 Query: 305 GDLMLVEVAQRIRSVMRAQDTVARLGGDEFVMLVDLKEPDDA--VTVADKLVKIINQSFN 362 GD +L VAQR+++ +R D VAR GGDEFV+++ + +A V +++ + + + Sbjct: 320 GDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAAVARPWQ 379 Query: 363 IEKHELRVSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRNGYHFFEASMNVNAHNQ 422 E +V+ASIG++ YP DG L+ +AD+AMY K GRN + FF M+ N+ Sbjct: 380 AGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDSQVSNR 439 Query: 423 LQWLQDLRLALERKEFILHYQPKYNSPNGPVLGAEALLRWQHPVHGMVGPDEFIGLAERS 482 L+ L +LR AL++ EF L+YQPK + +G ++GAEAL+RW+ P GMV PD FI AE + Sbjct: 440 LEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEA 499 Query: 483 GLIVPIGAWVLDEACRQMKVWYDIGHEDWKIAVNLSALQFAQTDLIDLVKDTLARHDLPA 542 GLIVPIG WVL ACRQ + W G +AVNLS Q Q+ L D V LA+ L A Sbjct: 500 GLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQS-LPDFVAGVLAQSGLAA 558 Query: 543 RCLTLEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYSSLLYLKRLPANELKIDR 602 L LE+TE+ M DAE S+ TL + LG+ IS+DDFGTGYSSL YL+R P + LKID+ Sbjct: 559 SWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDK 618 Query: 603 GFVRDLSDGNDDAAIISAIVALGRTLNLRIVAEGVETTKQQNFLTSVGCDALQGYLMGRP 662 FVRD+ D AAI+ AI++L LNLR++AEGVE +Q FL CD +QGY G+P Sbjct: 619 SFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKP 678 Query: 663 M 663 M Sbjct: 679 M 679 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 379 bits (974), Expect = e-106, Method: Compositional matrix adjust. Identities = 206/435 (47%), Positives = 278/435 (63%), Gaps = 5/435 (1%) Query: 231 SRTAKLARSLAEANEELTQLVLHDNLTKLPNRTLLDDRLNQAISKAAREQGHFALMFCDL 290 R L R + N++L HD LT LPNR LL DR+ A+ A R FA++F DL Sbjct: 242 QRNQVLKRIVGRTNDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMDL 301 Query: 291 DGFKAINDSLGHHVGDLMLVEVAQRIRSVMRAQDTVARLGGDEFVMLV-DLKEPDDAVTV 349 DGFKAINDSLGH VGD +LV VAQRIR +R +D VAR+GGDEFV++V +L PD + Sbjct: 302 DGFKAINDSLGHAVGDGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVEQL 361 Query: 350 ADKLVKIINQSFNIEKHELRVSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRNGYH 409 ++ ++ + Q F I+ LRV++SIGIA+YP G S L+ NADAAMY K+SGRN Y Sbjct: 362 SENILAALRQDFQIDDATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQSGRNTYR 421 Query: 410 FFEASMNVNAHNQLQWLQDLRLALERKEFILHYQPKYNSPNGPVLGAEALLRWQHPVHGM 469 FFE +M+ +A LQ Q L+ A++ ++F LH+QPKY + + G EALLRW HP G Sbjct: 422 FFEPAMHASAMRHLQVRQALQQAIDEQQFRLHFQPKYRGASKELTGLEALLRWTHPQLGE 481 Query: 470 VGPDEFIGLAERSGLIVPIGAWVLDEACRQMKVWYDIGHEDWKIAVNLSALQFAQTDLID 529 + P EFI +AERSG I+ IG WVL C Q+ W G + K+A+NLS LQ +TDL+ Sbjct: 482 MAPTEFIPIAERSGQILCIGDWVLQAVCEQIARWDAAGMKPVKVALNLSPLQM-RTDLVA 540 Query: 530 LVKDTLARHDLPARCLTLEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYSSLLY 589 + + + + + L E+TE+ AM D E S + + ++ LG DI+IDDFGTGYSSL Y Sbjct: 541 RIVELVGAAGIAPQRLMFEITETAAMKDVERSQRVIGELQALGFDIAIDDFGTGYSSLAY 600 Query: 590 LKRLPANELKIDRGFVRDLSDGNDDAA--IISAIVALGRTLNLRIVAEGVETTKQQNFLT 647 L + ++KIDR F L DG+D + I++AI+AL L + +VAEGVET Q L Sbjct: 601 LAQFHCRQIKIDRFFTSRL-DGDDHSGRAIVAAIIALAHALQMEVVAEGVETEGQLRELQ 659 Query: 648 SVGCDALQGYLMGRP 662 + CD +QG+L+GRP Sbjct: 660 LLHCDQVQGFLLGRP 674 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 378 bits (971), Expect = e-106, Method: Compositional matrix adjust. Identities = 192/438 (43%), Positives = 265/438 (60%), Gaps = 1/438 (0%) Query: 234 AKLARSLAEANEELTQLVLHDNLTKLPNRTLLDDRLNQAISKAAREQGHFALMFCDLDGF 293 A +A A+E + L +D LT LPNR L ++ AI+ A G A++ DLD F Sbjct: 653 ASVAIESRRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRF 712 Query: 294 KAINDSLGHHVGDLMLVEVAQRIRSVMRAQDTVARLGGDEFVMLVD-LKEPDDAVTVADK 352 K IND+ GH G+ +L E+AQR R+ +R D +AR+GGDEF++LVD +P +A K Sbjct: 713 KNINDTFGHETGEAVLQEIAQRFRNSLRELDILARVGGDEFIVLVDDFDDPVQLGEIAQK 772 Query: 353 LVKIINQSFNIEKHELRVSASIGIAIYPEDGSSRHDLVINADAAMYHTKRSGRNGYHFFE 412 L+ + F I+ E +S SIGIA YP DG + L+ NAD AMY K G++ Y FF Sbjct: 773 LLTEARKPFIIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFS 832 Query: 413 ASMNVNAHNQLQWLQDLRLALERKEFILHYQPKYNSPNGPVLGAEALLRWQHPVHGMVGP 472 +N N ++ +LR A+ER EF++HYQPK G ++GAEAL+RWQHP+ G++ P Sbjct: 833 DEINTNTVERIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPP 892 Query: 473 DEFIGLAERSGLIVPIGAWVLDEACRQMKVWYDIGHEDWKIAVNLSALQFAQTDLIDLVK 532 EFI LAE + LI IG VLD ACR + + +I++NL+ QF L+D +K Sbjct: 893 GEFITLAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIK 952 Query: 533 DTLARHDLPARCLTLEVTESTAMHDAESSLKTLQQIADLGVDISIDDFGTGYSSLLYLKR 592 + + RH P CL E+TES MH+ E ++ + I G ISIDDFGTGYSSL YLKR Sbjct: 953 EVVDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKR 1012 Query: 593 LPANELKIDRGFVRDLSDGNDDAAIISAIVALGRTLNLRIVAEGVETTKQQNFLTSVGCD 652 P N LKID+ F+ D+ D + +AI+ AI+A+ LNL++V EGVET KQ L G D Sbjct: 1013 FPVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSD 1072 Query: 653 ALQGYLMGRPMPPDKFIE 670 QGY +P+P +F++ Sbjct: 1073 EYQGYYFSKPVPYAQFVQ 1090 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,974,803 Number of extensions: 80909 Number of successful extensions: 421 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 345 Number of HSP's successfully gapped: 45 Length of query: 683 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 591 Effective length of database: 1,184,250 Effective search space: 699891750 Effective search space used: 699891750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)