BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0023.p (295 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 433 e-123 gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3... 116 2e-27 gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3... 115 4e-27 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 433 bits (1113), Expect = e-123, Method: Compositional matrix adjust. Identities = 216/301 (71%), Positives = 252/301 (83%), Gaps = 7/301 (2%) Query: 2 SDDFFHLTALPKRSVVLGGGYIAVELASILNGLGSEVTLVYRGKSLLRGMDSELGIFLAE 61 SDDFFHL ALP+R+VVLGGGYIAVELASILNGLG EVTLVYR + LLR MD++LGI LA+ Sbjct: 158 SDDFFHLKALPQRAVVLGGGYIAVELASILNGLGCEVTLVYRRERLLRNMDADLGIHLAD 217 Query: 62 EMRKKGMNILFENNIDAIASDGAG-------KRVKLSDGKTVDADCVLFATGRHPNTAGL 114 EM KKG+ I+F +I+AI ++ AG K V+L++G+ + A CVLFATGR NTAGL Sbjct: 218 EMAKKGIRIVFNASIEAIEAEQAGEASEASVKTVRLTNGEALSAQCVLFATGRTANTAGL 277 Query: 115 GLEKVGVALTAKGAVKVDQHFVSDVASIHAIGDVIDRVALTPVALAEGMVVAARLFNQGG 174 GL++ GV L A GA++V+Q F S V SI A+GDVIDRVALTPVALAE M V +RLF +G Sbjct: 278 GLQEAGVKLKANGAIEVNQDFESSVPSILAVGDVIDRVALTPVALAEAMAVVSRLFGKGE 337 Query: 175 REMSYENIPTAVFSHPNVGTVGLGEEDARKKVGEVRIFKSEFKALKHTLSGNAERTFMKL 234 R MSY NIPTAVFSHPNVGTVGL EE+AR++ GE+RIFK++FK LK+TLS N ERTFMKL Sbjct: 338 RGMSYANIPTAVFSHPNVGTVGLSEEEARQQFGELRIFKTDFKPLKNTLSRNTERTFMKL 397 Query: 235 VVDAKNDRVLGVHMVGADAGEIVQGFAVALQCGATKAQFDATIGIHPTSAEEFVTMRTPV 294 VVDA+ DRVLGVHMVG DAGE++QGFAVALQCGATKAQFD TIGIHPTSAEEFVT+RTPV Sbjct: 398 VVDARTDRVLGVHMVGGDAGEVIQGFAVALQCGATKAQFDTTIGIHPTSAEEFVTLRTPV 457 Query: 295 A 295 A Sbjct: 458 A 458 >gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 87/294 (29%), Positives = 147/294 (50%), Gaps = 18/294 (6%) Query: 6 FHLTALPKRSVVLGGGYIAVELASILNGLGSEVTLVYRGKSLLRGMDSELGIFLAEEMRK 65 L +PKR +V+GGG I +E+A++ + LG+ + +V L++G D ++ + K Sbjct: 289 LELRQVPKRMLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEK 348 Query: 66 KGMNILFENNIDAIASDGAGKRVKLSD---GKTVDA----DCVLFATGRHPNTAGLGLEK 118 + N++ + A+ + G +V G T D VL A GR PN L +K Sbjct: 349 RFDNVMVKTKTVAVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADK 408 Query: 119 VGVALTAKGAVKVDQHFVSDVASIHAIGDVIDRVALTPVALAEGMVVAARLFNQGGREMS 178 GV ++ +G + VD+ ++V I AIGD++ + L A+ EG V A + E S Sbjct: 409 AGVIVSDRGFIAVDKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAG----EKS 464 Query: 179 YEN---IPTAVFSHPNVGTVGLGEEDARKKVGEVRIFKSEFK--ALKHTLSGNAERTFMK 233 + + IP+ ++ P V VG+ E++A+ K ++I K F A ++ F K Sbjct: 465 FFDASVIPSVAYTDPEVAWVGVTEDEAKAK--GIKIEKGHFPWAASGRAVANGRSEGFTK 522 Query: 234 LVVDAKNDRVLGVHMVGADAGEIVQGFAVALQCGATKAQFDATIGIHPTSAEEF 287 L+ DA+ R++G +VG AG+++ A+A++ GA TI HPT E Sbjct: 523 LLFDAETHRIIGGGIVGTHAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESL 576 >gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 115 bits (289), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 80/282 (28%), Positives = 142/282 (50%), Gaps = 5/282 (1%) Query: 8 LTALPKRSVVLGGGYIAVELASILNGLGSEVTLVYRGKSLLRGMDSELGIFLAEEMRKKG 67 +T +PK+ V+G G I +E+ S+ LG+EVT++ + L +D ++ + + K+G Sbjct: 179 ITEVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLTKQG 238 Query: 68 MNILFENNIDAIASDGAGKRVKLSDGK----TVDADCVLFATGRHPNTAGLGLEKVGVAL 123 + + I AI + V+ +D K T + D ++ + GR PNT GL E VG+ L Sbjct: 239 LAVNLGVKIGAIKAGKNSVSVEYTDAKGDAHTGEFDKLIVSIGRTPNTIGLNAEGVGLKL 298 Query: 124 TAKGAVKVDQHFVSDVASIHAIGDVIDRVALTPVALAEGMVVAARLFNQGGREMSYENIP 183 +G + VD +++ ++ A+GDV+ L A EG+ VA R+ Q G +++ IP Sbjct: 299 DERGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGH-VNFNTIP 357 Query: 184 TAVFSHPNVGTVGLGEEDARKKVGEVRIFKSEFKALKHTLSGNAERTFMKLVVDAKNDRV 243 +++ P + VG E+ + + + + F A + +K + DAK D + Sbjct: 358 WVIYTSPEIAWVGKTEQQLKAEGVQYKAGTFPFLANGRARALGDTSGMVKFLADAKTDEI 417 Query: 244 LGVHMVGADAGEIVQGFAVALQCGATKAQFDATIGIHPTSAE 285 LGVH+VG A E++ VA++ A+ HP+ +E Sbjct: 418 LGVHIVGPMASELISEAVVAMEFRASSEDIARICHAHPSLSE 459 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 914,002 Number of extensions: 39576 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 122 Number of HSP's successfully gapped: 4 Length of query: 295 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 210 Effective length of database: 1,217,395 Effective search space: 255652950 Effective search space used: 255652950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)