BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0025.0010.p
         (465 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310540|ref|YP_003774632.1| nitrate transporter periplasmic...   229   3e-61

>gi|300310540|ref|YP_003774632.1| nitrate transporter periplasmic
           protein [Herbaspirillum seropedicae SmR1]
          Length = 414

 Score =  229 bits (585), Expect = 3e-61,   Method: Compositional matrix adjust.
 Identities = 134/378 (35%), Positives = 206/378 (54%), Gaps = 29/378 (7%)

Query: 108 EKKDLKIGFIAITCAAPLIMADPLGFYKKQGLNVSLNKTAGWALIRDKMINKEHDASHFL 167
           EK+++KIGFI +T  A ++MA  LG  KK G+ +  +K A WA +RDK+ N E DA+H L
Sbjct: 42  EKEEVKIGFIPLTDCASVVMASVLGLDKKYGIKIIPSKEASWAGVRDKLTNGELDAAHVL 101

Query: 168 SPMPLAMSMGVGSNPVQMRVATIQNINGQAITLHVKHKDK-------------RDPKQWK 214
             +   + +G+G     M V    N NGQAITL  K  DK              D ++  
Sbjct: 102 YGLIYGVQLGIGGQKKDMAVLMGLNHNGQAITLSRKLADKGAVDGASLYKLMQTDKRE-- 159

Query: 215 GFRFAVPFEYSMHNFLLRYYLAENGIDPDRDVQIRVTPPPEMVANLRAGNIDGFLGPDPF 274
            + FA  F    H   L Y+LA NGI+P +D ++   PPP+MVAN+R GN+DGF   +P+
Sbjct: 160 -YTFAQTFPTGTHAMWLYYWLAANGINPMKDAKVITVPPPQMVANMRVGNMDGFCVGEPW 218

Query: 275 NQRAVYDEVGFIHILSKEIWDGHPCCAFGMSEDFVKQNPNTFAALFRAVLTAAA-MARDP 333
             RA+ D VG     +++IW  HP    G S +FVK+ PNT  A+  A+L A+  +    
Sbjct: 219 GHRAIVDGVGITATTTQDIWKDHPEKVLGSSLEFVKKYPNTCRAMMAAILEASKWIDASL 278

Query: 334 ANRSLVAKVISPAAYLNQPETVVEQVLTGRFADGLGNVKNVP--------GRADFDPVPW 385
            N++ +A+VI+  +Y+N  + V++Q + GR+ +GLG   + P        G+ +F   P+
Sbjct: 279 TNKNRMAEVIADKSYVNTSKDVIDQRILGRYQNGLGKTWDDPNYMKFYEDGQVNF---PF 335

Query: 386 DSMAVWILSQMKRWGYVKGEVDYKGISERVLLLTDAKKYMKELGQPVPDGAYRKYKIMDK 445
            S  +W ++Q +RWG +K + DY  +++ V  +   K+     G P+P  + R  ++MD 
Sbjct: 336 LSDGMWFMTQHRRWGLLKSDPDYLAVAKAVNQIDLYKQAAAMTGTPLPKDSMRSSRLMDG 395

Query: 446 -EFDPAKADAYVNSFAIK 462
             +D     AY  SF IK
Sbjct: 396 VVWDGKNPAAYAQSFKIK 413


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.135    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,411,554
Number of extensions: 56576
Number of successful extensions: 157
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 155
Number of HSP's successfully gapped: 1
Length of query: 465
Length of database: 1,619,870
Length adjustment: 89
Effective length of query: 376
Effective length of database: 1,198,455
Effective search space: 450619080
Effective search space used: 450619080
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)