BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0006.p (141 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 263 3e-72 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 103 4e-24 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 100 8e-23 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 93 8e-21 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 89 2e-19 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 263 bits (673), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 127/141 (90%), Positives = 132/141 (93%) Query: 1 VQINARSVVAFTRAVIPVMRKQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTITRGM 60 + INARSVVAFTRAVIPVMR QGGGNIINVTSVAARHGGGPGALIYAASKGFVST+TRGM Sbjct: 113 ININARSVVAFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGM 172 Query: 61 AKELQPDNIRVNAVAPGVIMTPFQERFTTPEQLESFRKTIPMGRIGDPAECDGAFLYLAS 120 AKEL D IRVNAVAPGVIMTPFQERFTTPEQLE FRKTIPMGRIG+P EC GAFLYLAS Sbjct: 173 AKELLADKIRVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLAS 232 Query: 121 DALSGYVTGQIIDVNGGQYMP 141 + +SGYVTGQIIDVNGGQYMP Sbjct: 233 EQMSGYVTGQIIDVNGGQYMP 253 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 103 bits (258), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 55/141 (39%), Positives = 86/141 (60%), Gaps = 2/141 (1%) Query: 1 VQINARSVVAFTRAVIPVMRKQGGGNIINVTSVAARHGGG-PGALIYAASKGFVSTITRG 59 + ++ R + ++AV+P M+ Q G+I+ ++SV+A+ GGG G Y+A+K V + R Sbjct: 109 LDVSLRGTLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARA 168 Query: 60 MAKELQPDNIRVNAVAPGVIMTPFQERFTTPEQLESFRKTIPMGRIGDPAECDGAFLYLA 119 MA+E P IRVN + PG+I T + + E+ +TIP+GR+G P + G ++LA Sbjct: 169 MAREFGPQGIRVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLA 228 Query: 120 SDALSGYVTGQIIDVNGGQYM 140 SD LS Y TG +DVNGG + Sbjct: 229 SD-LSQYCTGVTLDVNGGMLI 248 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 99.8 bits (247), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 56/141 (39%), Positives = 86/141 (60%), Gaps = 2/141 (1%) Query: 1 VQINARSVVAFTRAVIPVMRKQGGGNIINVTSVAARHGGG-PGALIYAASKGFVSTITRG 59 + ++ R + ++AV+P MR Q G+II ++SV+A+ GGG G Y+A+K V + R Sbjct: 109 LDVSLRGTLYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARA 168 Query: 60 MAKELQPDNIRVNAVAPGVIMTPFQERFTTPEQLESFRKTIPMGRIGDPAECDGAFLYLA 119 MA+E P+ IRVN++ PG+I T + T E+ +TIP+ R+G + GA ++L Sbjct: 169 MAREFGPEGIRVNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLG 228 Query: 120 SDALSGYVTGQIIDVNGGQYM 140 SD LS Y TG +DVNGG + Sbjct: 229 SD-LSQYCTGITLDVNGGMLI 248 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 93.2 bits (230), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 57/140 (40%), Positives = 74/140 (52%), Gaps = 6/140 (4%) Query: 3 INARSVVAFTRAVIPVMRKQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTITRGMAK 62 +N +S+ + +P R+ GGG IN+ S A PG Y SKG V T ++ MA Sbjct: 113 VNVKSIFLSAKTFVPYFRQVGGGAFINIASTAGIRPR-PGLTWYNGSKGAVITTSKSMAA 171 Query: 63 ELQPDNIRVNAVAPGVIMTPFQERF----TTPEQLESFRKTIPMGRIGDPAECDGAFLYL 118 EL PD IRVN V P + T F TPE + F TIPMGR P + A LYL Sbjct: 172 ELGPDKIRVNCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYL 231 Query: 119 ASDALSGYVTGQIIDVNGGQ 138 SD + +VTG I+V+GG+ Sbjct: 232 GSDE-AEFVTGVCIEVDGGR 250 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 88.6 bits (218), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 56/140 (40%), Positives = 79/140 (56%), Gaps = 3/140 (2%) Query: 1 VQINARSVVAFTRAVIPVMRKQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTITRGM 60 + N +V +RAV+ M K G IIN+TSV G PG + YAA+K V+ ++R + Sbjct: 114 IATNLTAVGRLSRAVLRGMMKARTGRIINITSVVG-EAGNPGQMNYAAAKAGVAGMSRAL 172 Query: 61 AKELQPDNIRVNAVAPGVIMTPFQERFTTPEQLESFRKTIPMGRIGDPAECDGAFLYLAS 120 A+E+ NI VN VAPG I T R +Q + + IP+GR+G E A +LAS Sbjct: 173 AREIGSRNITVNCVAPGFIDTDM-TRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLAS 231 Query: 121 DALSGYVTGQIIDVNGGQYM 140 + Y+TG ++VNGG YM Sbjct: 232 PQ-AAYITGTTLNVNGGMYM 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 480,736 Number of extensions: 19876 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 34 Length of query: 141 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 65 Effective length of database: 1,260,010 Effective search space: 81900650 Effective search space used: 81900650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)