BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0004.p (131 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312613|ref|YP_003776705.1| transcription regulator protein... 134 2e-33 >gi|300312613|ref|YP_003776705.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 239 Score = 134 bits (337), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 65/82 (79%), Positives = 73/82 (89%) Query: 50 AVREXFLQSIAGSLYLLGKKSRTITSRIPATLERSLQDHRDILIALKARDPQQASDAMRT 109 A FLQSIAGSLY+LGKKSR+ITSRIPATLERSLQDHR IL ALKARDPQ+AS+AMR Sbjct: 158 ACNNPFLQSIAGSLYMLGKKSRSITSRIPATLERSLQDHRQILDALKARDPQKASEAMRH 217 Query: 110 HLIRVRDAYRGAAQNKDESVHD 131 HL+RVRDAYR A +N++ESVHD Sbjct: 218 HLMRVRDAYRSAGRNRNESVHD 239 Score = 106 bits (265), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 52/53 (98%), Positives = 52/53 (98%) Query: 1 MSFDPIVQKSISEQVAQRLLTMIRSGLLKPGQQLPPERELAAMLGVGRPAVRE 53 MSFDPIVQKSISEQVAQRLLTMIRSGLLKP QQLPPERELAAMLGVGRPAVRE Sbjct: 1 MSFDPIVQKSISEQVAQRLLTMIRSGLLKPDQQLPPERELAAMLGVGRPAVRE 53 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 373,456 Number of extensions: 13857 Number of successful extensions: 91 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 2 Length of query: 131 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 56 Effective length of database: 1,264,745 Effective search space: 70825720 Effective search space used: 70825720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)