BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0002.p (329 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 166 2e-42 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 159 3e-40 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 149 3e-37 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 124 8e-30 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 84 1e-17 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 166 bits (421), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 110/342 (32%), Positives = 177/342 (51%), Gaps = 35/342 (10%) Query: 14 LEYRKLGNSAIEVAPLAFGGNVFGWTADEATSFSLLDKFVDAGLNLVDTADVYSRWASGN 73 ++YR LG + ++V+ + G +G EA + + LD V+ G+NL+D A++Y Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 74 KGGESETIIGKWIRQGGRRDKLVIATKVGMDMGDGKVGLSPAYIR------------RAV 121 G +E +G W+++ G+RD+L+IATK G + +P ++R A+ Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKC---TGPARKPHNPRHVRGGINNLDRKGLTEAL 117 Query: 122 EDSLTRLQTDYIDLYQSH-----------------TDDKNTPQEETLHTYGELVQQGKVR 164 SL RLQ D++DLYQ H D+ P EETL E V+ GK+R Sbjct: 118 HGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIR 177 Query: 165 LIGASNFGAGRLEEARKLSREHGYPLYQTLQPEYNLYSRADFENNLEQYCLKEKIGVINY 224 IG SN A + + + + +HG T+Q YNL +R+ FE NL ++ +E++G++ Y Sbjct: 178 TIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRS-FEINLAEFAHREQVGLLAY 236 Query: 225 YSLASGFLSGKYR--SEKDLGKSTRGGKTQSYLNPRGLRILDALDAVAERYGSTPAAVAL 282 LA G LSGKY + G+ T + Y N + R A+A ++G PA +AL Sbjct: 237 SPLAFGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMAL 296 Query: 283 AWLIARPSITAPIASATTLEQMDALIAATRLSLDAAAIARLD 324 A++ +RP +T+ I ATTL Q+ + I + L L +A ++ Sbjct: 297 AYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIE 338 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 159 bits (402), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 105/323 (32%), Positives = 163/323 (50%), Gaps = 17/323 (5%) Query: 14 LEYRKLGNSAIEVAPLAFGGNVFGWTADEATSFSLLDKFVDAGLNLVDTADVYSRWASGN 73 + YR+LG S ++V+ L G +FG D S ++ + G+N +DTADVY++ Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTK----- 55 Query: 74 KGGESETIIGKWIRQGGRRDKLVIATKVGMDMG--DGKVGLSPAYIRRAVEDSLTRLQTD 131 G+SE I+GK + G+R + V+A+K+G M + S ++I R E+ LTRL TD Sbjct: 56 --GKSEEIVGKLL--AGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTD 111 Query: 132 YIDLYQSHTDDKNTPQEETLHTYGELVQQGKVRLIGASNFGAGRLEEARKLSREHGYPLY 191 Y+D+ H D ++ EE + G+L++ GK+R G SNF R+ E +L ++ PL Sbjct: 112 YLDILYMHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLP 171 Query: 192 QTLQPEYNLYSRADFENNLEQYCLKEKIGVINYYSLASGFLSGKYRSEKDLGKSTRGGK- 250 QP YNL +R E + C +GV+ Y +A G L+GKY+ + + TR G+ Sbjct: 172 LVCQPYYNLLNRQP-EVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRG 230 Query: 251 ----TQSYLNPRGLRILDALDAVAERYGSTPAAVALAWLIARPSITAPIASATTLEQMDA 306 ++ L I L E G A AW++A +I++ IA TL QM+ Sbjct: 231 DKRMMETEFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMED 290 Query: 307 LIAATRLSLDAAAIARLDQASAP 329 A L + A A +D P Sbjct: 291 YYDAAELKISAEEEALVDSLVTP 313 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 149 bits (376), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 104/290 (35%), Positives = 157/290 (54%), Gaps = 27/290 (9%) Query: 17 RKLGNSAIEVAPLAFGG---NVFGWTADEATSFSLLDKFVDAGLNLVDTADVYSRWASGN 73 R+LG++ V+ + G + F D+A S + +D+ ++ G+NL+DTAD+Y + + Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTN-- 75 Query: 74 KGGESETIIGKWIRQGGRRDKLVIATKVGM-----DMGDGKVGLSPAYIRRAVEDSLTRL 128 E ++G+ I+ GRRDK IATK G+ D G V SPAYIR+AVE SL RL Sbjct: 76 -----EELLGRAIK--GRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRL 128 Query: 129 QTDYIDLYQSHTDDKNTPQEETLHTYGELVQQGKVRLIGASNFGAGRLEEARKLSREHGY 188 + IDLY H D TP E T+ +LVQ GK+R IG S A LE A ++ + Sbjct: 129 GVETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSEASAATLERAHRV-----H 183 Query: 189 PLYQTLQPEYNLYSRADFENNLEQYCLKEKIGVINYYSLASGFLSGKYRSEKDLGKS--- 245 P+ LQ EY+L++R D E + C IG + Y L GFL+G + +DL + Sbjct: 184 PV-TALQSEYSLWTR-DPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFR 241 Query: 246 TRGGKTQSYLNPRGLRILDALDAVAERYGSTPAAVALAWLIARPSITAPI 295 + Q R L++ + + +A ++G TP+ +ALAW++A+ PI Sbjct: 242 RSNPRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPI 291 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 124 bits (312), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 97/328 (29%), Positives = 160/328 (48%), Gaps = 28/328 (8%) Query: 13 QLEYRKLGNSAIEVAPLAFG-GNVFGWTADEATSFSLLDKFVDAGLNLVDTADVYSRWAS 71 ++ YR+ G S + + ++ G FG + T ++L + D G+ D A+ Y Sbjct: 13 RMPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNY----- 67 Query: 72 GNKGGESETIIGKWIRQG--GRRDKLVIATKVGMDMGDGKVGLSPAYIRR----AVEDSL 125 G G +E G+W+ + RD+LVI++K G M G G P+ R+ + E SL Sbjct: 68 GPPAGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYG-GPSGARKHLIASCEQSL 126 Query: 126 TRLQTDYIDLYQSHTDDKNTPQEETLHTYGELVQQGKVRLIGASNFGAGRLEEARKLSRE 185 R+ DY+D++ SH D +TP EET+ L +QGK +G S++ +A + + Sbjct: 127 KRMGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKS 186 Query: 186 HGYPLYQTLQPEYNLYSRADFENNLEQYCLKEKIGVINYYSLASGFLSGKYRSEKDLGKS 245 G PL QP Y++ +RA E L + +G I + LA G L+ KY LG Sbjct: 187 EGVPLL-IHQPNYSMLNRA-IEGGLLPALEELGVGCIGFSPLAQGLLTSKY-----LGGV 239 Query: 246 TRGGKT-------QSYLNPRGLRILDALDAVAERYGSTPAAVALAWLIARPSITAPIASA 298 G + Q L+ L + AL+ +A + G T A +A+AW++ +T+ + A Sbjct: 240 PEGARVTRAPSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGA 299 Query: 299 TTLEQM-DALIAATRLSLDAAAIARLDQ 325 T+EQ+ D+L A +L +A +D+ Sbjct: 300 RTVEQLDDSLDAVNKLDFTQEELASIDK 327 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 88/283 (31%), Positives = 128/283 (45%), Gaps = 53/283 (18%) Query: 49 LDKFVDAGLNLVDTADVYSRWASGNKGGESETIIGKWIRQGGRRDKLVIATKVGMDMGDG 108 L +D G++L+DTA++Y+ G +E I+ K I GRRD++ + +KV Sbjct: 39 LQAGLDLGMSLIDTAEMYAE-------GGAERIVAKAI--AGRRDQVYLVSKVLPHNASK 89 Query: 109 KVGLSPAYIRRAVEDSLTRLQTDYIDLYQSHTDDKNTPQEETLHTYGELVQQGKVRLIGA 168 + ++ A E SL RL TD IDLY H + P T+ LV QGK+ G Sbjct: 90 RGTIA------ACEASLKRLGTDRIDLYLLHWRGAH-PLAATIEGMETLVSQGKIGGWGV 142 Query: 169 SNFGAGRLEEARKLSREHGYPLYQTLQPEYNLYSRADFENNLEQYCLKEKIGVINYYSLA 228 SNF +++ L HG Y Q YNL SR E +L + I V+ Y + Sbjct: 143 SNFDTDDIDDL--LEEPHGEH-YLCNQVLYNL-SRRGIEYDLLPQAQQAHIAVMAYSPIE 198 Query: 229 SGFLSGKYRSEKDLGKSTRGGKTQSYLNPRGLRILD--ALDAVAERYGSTPAAVALAWLI 286 G RIL AL A+A+ + TPA +ALAW++ Sbjct: 199 QG------------------------------RILKHPALTALAQAHRVTPAQIALAWVL 228 Query: 287 ARPSITAPIASATTLEQMDALIAATRLSLDAAAIARLDQASAP 329 +P + A I A + + A A+ +SL AA + LD+A AP Sbjct: 229 RQPGVVA-IPKAADVAHVQANRASLDISLSAADLKILDEAFAP 270 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,113,101 Number of extensions: 51147 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 6 Length of query: 329 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 243 Effective length of database: 1,212,660 Effective search space: 294676380 Effective search space used: 294676380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)