BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0048.p (434 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillu... 859 0.0 gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbasp... 182 4e-47 gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillu... 75 1e-14 >gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 859 bits (2219), Expect = 0.0, Method: Compositional matrix adjust. Identities = 406/433 (93%), Positives = 420/433 (96%) Query: 1 MPTSDIKATLSFSDGTPSLDFPVYKGTIGPDVIDIRKLYGATGKFTYDPGFMSTAACNSS 60 M +DIKATLSFSDG+PS+DFPVYKG+IGPDVIDIRKLYGATGKFTYDPGFMSTA+CNSS Sbjct: 24 MSNTDIKATLSFSDGSPSVDFPVYKGSIGPDVIDIRKLYGATGKFTYDPGFMSTASCNSS 83 Query: 61 ITYIDGDKGELLYRGYPIEQLAVNCDFLETCYLLLHGELPTATQKEEFVETVTQHTMVHE 120 ITYIDGDKGELLYRGYPIEQLAVNCDFLE+CYLLLHGELP A QK+ FV TVTQHTMVHE Sbjct: 84 ITYIDGDKGELLYRGYPIEQLAVNCDFLESCYLLLHGELPNAEQKKVFVNTVTQHTMVHE 143 Query: 121 QMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISNPVHRDVSAIRMIAKLPTLVAMA 180 QMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISN HR+VSAIRMIAKLPTLVAMA Sbjct: 144 QMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISNANHREVSAIRMIAKLPTLVAMA 203 Query: 181 YKYNIGQPFVYPRNDLSYSANFMRMMFANPCEEYKVNEVLVRALDRILILHADHEQNAST 240 YKY+IGQPFVYPRNDLSY+ANFMRMMFANPCEEY+VNEVLVRALDRILILHADHEQNAST Sbjct: 204 YKYSIGQPFVYPRNDLSYTANFMRMMFANPCEEYQVNEVLVRALDRILILHADHEQNAST 263 Query: 241 STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALTMLEGIGSVDNIPEFIKQVKDKN 300 STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAAL MLE IGSVD IPEFIKQVKDKN Sbjct: 264 STVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSVDKIPEFIKQVKDKN 323 Query: 301 SGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALEDDYFVS 360 SGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALED+YFVS Sbjct: 324 SGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALEDEYFVS 383 Query: 361 RKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTVGWIAQWNEMISDPEQKIGRPRQLFV 420 RKLYPNVDFYSGIVQRALGIPTSMFTCIFAMART+GWIAQWNEMISDPEQKIGRPRQLFV Sbjct: 384 RKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEMISDPEQKIGRPRQLFV 443 Query: 421 GSAKRDVSPIDKR 433 GS +RDV PIDKR Sbjct: 444 GSPRRDVPPIDKR 456 >gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbaspirillum seropedicae SmR1] Length = 384 Score = 182 bits (462), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 121/379 (31%), Positives = 195/379 (51%), Gaps = 18/379 (4%) Query: 56 ACNSSITYIDGDKGELLYRGYPIEQLAVNCDFLETCYLLLHGELPTATQKEEFVETVTQH 115 A N+++ + +L YRGY I +A +C+F E +LL+HG+LPTA + + + Sbjct: 22 AGNTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAHLLVHGKLPTAAELRAYKAKLKSL 81 Query: 116 TMVHEQMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISNPVHRDVSAIRMIAKLPT 175 + + +HPM V+ V AL + D + P RD+ A R++A L + Sbjct: 82 RGLPANVKAALEWLPAASHPMDVMRTGVSALGCVLPEKDDHNTPGARDI-ADRLMASLGS 140 Query: 176 LVAMAYKY-NIGQPFVYPRNDLSYSANFMRMMFANPCEEYKVNEVLVRALDRILILHADH 234 ++ Y Y + G +D S A+F+ ++ K +E +A+ LIL+A+H Sbjct: 141 MLLYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGE-----KPSEAWEKAMHTSLILYAEH 195 Query: 235 EQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALTMLEGIGSVDNIPEFIK 294 E NAST T R+ +G++ ++ I I L GP HGGANE A + + + D I+ Sbjct: 196 EFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEVAFEIQKRYDNPDEAEADIR 255 Query: 295 QVKDKNSGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALE 354 + + +N V ++GFGH VY DPR K+++E ++ E G +F +A LE + + Sbjct: 256 R-RVENKEV-VIGFGHPVYTISDPRNKVIKEVARKLSKEAG--STKMFDIAERLETVMWD 311 Query: 355 DDYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTVGWIAQWNEMISDPEQKIGR 414 +K++PN+D++S + +G+PT+MFT +F +ART GW A E D KI R Sbjct: 312 -----IKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRID--NKIIR 364 Query: 415 PRQLFVGSAKRDVSPIDKR 433 P +VG PI KR Sbjct: 365 PSANYVGPEDLKFVPISKR 383 >gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 59/200 (29%), Positives = 95/200 (47%), Gaps = 23/200 (11%) Query: 228 LILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALTMLEGIGSVD 287 L+L ADHE NAS+ T R S+ A+ A + AG+A L G HG + + +G+ D Sbjct: 217 LVLCADHELNASSFTSRCVASTDASLRAVVIAGLAGLSGGRHGATTGRVEALWDELGTRD 276 Query: 288 NIPEFIKQVKDKNS-GVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAM 346 ++++++ S G L GFGH +Y + DPRA + L+++ P Sbjct: 277 ----VDRKMRERLSRGDDLPGFGHTLYPDGDPRASYL-------LSQILPRHSPW----- 320 Query: 347 ELEKIALEDDYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTVGWIAQWNEMIS 406 A+ D P+VDF ++R L +P +FA+ R++GWIA E Sbjct: 321 ----AAMIDAGITLTGQKPSVDFALVALRRHLRLPVGAAFGLFALGRSIGWIAHGLEQRE 376 Query: 407 DPEQKIGRPRQLFVGSAKRD 426 + + RPR +++G D Sbjct: 377 --QHHLIRPRAVYIGPQPED 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,346,078 Number of extensions: 54929 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 3 Length of query: 434 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 346 Effective length of database: 1,203,190 Effective search space: 416303740 Effective search space used: 416303740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)