BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0033.p (131 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312287|ref|YP_003776379.1| chemotaxis cheY protein [Herbas... 229 4e-62 gi|300309955|ref|YP_003774047.1| two-component system sensor his... 124 3e-30 gi|300311034|ref|YP_003775126.1| chemotaxis response regulator p... 100 5e-23 gi|300311785|ref|YP_003775877.1| chemotaxis response regulator C... 96 1e-21 gi|300311339|ref|YP_003775431.1| CheY chemotaxis protein [Herbas... 91 3e-20 >gi|300312287|ref|YP_003776379.1| chemotaxis cheY protein [Herbaspirillum seropedicae SmR1] Length = 121 Score = 229 bits (585), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 113/121 (93%), Positives = 116/121 (95%) Query: 11 MSKTILAVDDSGSLRQMVVFSLKAAGYQVTEAVDGQEALDKAKLQTFDLVLTDQNMPRMD 70 MSKTILAVDDSGSLRQMVVFSLKAAGY VTEAVDG +AL+KAK QTFDLVLTDQNMPRMD Sbjct: 1 MSKTILAVDDSGSLRQMVVFSLKAAGYNVTEAVDGVDALEKAKSQTFDLVLTDQNMPRMD 60 Query: 71 GLTLIRSLRALTSYQRVPILMLTTEFSDEMKAKGKAAGANGWLVKPFDPQRLTEVVKKVI 130 GLTLIRSLR LTSY RVPILMLTTEFSDEMKAKGKAAGANGWLVKPFDPQRLTEVV+KVI Sbjct: 61 GLTLIRSLRELTSYARVPILMLTTEFSDEMKAKGKAAGANGWLVKPFDPQRLTEVVRKVI 120 Query: 131 G 131 G Sbjct: 121 G 121 >gi|300309955|ref|YP_003774047.1| two-component system sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 122 Score = 124 bits (310), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 58/120 (48%), Positives = 82/120 (68%) Query: 11 MSKTILAVDDSGSLRQMVVFSLKAAGYQVTEAVDGQEALDKAKLQTFDLVLTDQNMPRMD 70 M K IL VDDS SLRQ + +LK+AGY V EA DG++AL + F+LV++D NMP +D Sbjct: 1 MEKNILIVDDSFSLRQTISAALKSAGYHVEEAADGKQALAHLDGRKFNLVISDLNMPHLD 60 Query: 71 GLTLIRSLRALTSYQRVPILMLTTEFSDEMKAKGKAAGANGWLVKPFDPQRLTEVVKKVI 130 GL+ +R ++ + +Y+ P++MLTTE S+ K +GKAAG W+ KPF P L + V K+I Sbjct: 61 GLSFVREMKQMPAYKFTPVIMLTTESSEARKQEGKAAGIRAWVHKPFQPPVLLDAVAKLI 120 >gi|300311034|ref|YP_003775126.1| chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 126 Score = 100 bits (248), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 56/120 (46%), Positives = 77/120 (64%), Gaps = 1/120 (0%) Query: 12 SKTILAVDDSGSLRQMVVFSLKAAGYQVTEAVDGQEALDKAKL-QTFDLVLTDQNMPRMD 70 SK+I VDDS ++ V L+ AG+ V A DG +AL K + DL++TD NMP MD Sbjct: 4 SKSIFLVDDSATMLMSVRSHLEMAGFAVDTAEDGVKALKKMQAGYKPDLLITDINMPNMD 63 Query: 71 GLTLIRSLRALTSYQRVPILMLTTEFSDEMKAKGKAAGANGWLVKPFDPQRLTEVVKKVI 130 GL LIR++RAL ++ +PIL LTTE + +GK AGA GWLVKP L ++V++V+ Sbjct: 64 GLELIRNVRALPGFRFIPILTLTTESQSGKRDEGKKAGATGWLVKPVGGADLLKIVRQVL 123 >gi|300311785|ref|YP_003775877.1| chemotaxis response regulator CheY [Herbaspirillum seropedicae SmR1] Length = 122 Score = 95.9 bits (237), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 55/118 (46%), Positives = 71/118 (60%), Gaps = 1/118 (0%) Query: 15 ILAVDDSGSLRQMVVFSLKAAGYQVTEAVDGQEALDKAKLQ-TFDLVLTDQNMPRMDGLT 73 IL VDDS ++R V LK G V AVDG++ L K K LV++D NMP MDGLT Sbjct: 4 ILVVDDSSTVRAEVGDFLKKNGLDVALAVDGRDGLSKLKGDPAIKLVVSDVNMPNMDGLT 63 Query: 74 LIRSLRALTSYQRVPILMLTTEFSDEMKAKGKAAGANGWLVKPFDPQRLTEVVKKVIG 131 + +R+ V I+MLTTE S MK +GKAAG GW+VKPF + + KK++G Sbjct: 64 MAEKIRSELGNASVNIIMLTTENSPIMKERGKAAGIKGWIVKPFKGEAVLPTFKKLVG 121 >gi|300311339|ref|YP_003775431.1| CheY chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 131 Score = 90.9 bits (224), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 49/117 (41%), Positives = 70/117 (59%), Gaps = 1/117 (0%) Query: 15 ILAVDDSGSLRQMVVFSLKAAGY-QVTEAVDGQEALDKAKLQTFDLVLTDQNMPRMDGLT 73 L VDD ++R++V LK GY V EA DG +AL K + FD V++D NMP MDGLT Sbjct: 8 FLVVDDFSTMRRIVRNLLKELGYTNVDEAEDGVQALQKLRSDQFDFVVSDWNMPNMDGLT 67 Query: 74 LIRSLRALTSYQRVPILMLTTEFSDEMKAKGKAAGANGWLVKPFDPQRLTEVVKKVI 130 +++ +R + ++P+LM+T E E AGANG++VKPF L E + K+ Sbjct: 68 MLQEIRKDPALSKLPVLMVTAEAKKENIVAAAQAGANGYVVKPFTAATLDEKLGKIF 124 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 302,478 Number of extensions: 9256 Number of successful extensions: 156 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 44 Length of query: 131 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 56 Effective length of database: 1,264,745 Effective search space: 70825720 Effective search space used: 70825720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)