BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0025.p (381 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 589 e-169 gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 112 5e-26 gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 99 8e-22 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 589 bits (1519), Expect = e-169, Method: Compositional matrix adjust. Identities = 285/383 (74%), Positives = 321/383 (83%), Gaps = 11/383 (2%) Query: 6 TPQTSLIHSKYAAPDNFGALPVAINHASTVLFPNVAAMRARNWKEKSGYTYGLHGTPTTF 65 TPQTSL+HS Y APD F A PV I+HASTVLFP+VAAMR+R+W+EK GYTYGLHGTPT+F Sbjct: 10 TPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTYGLHGTPTSF 69 Query: 66 TLEARLAEIEGGRHCRIVPSGLAAIAMVNLALLKSGDDVLLPDNVYGPNRDQVVWLERDF 125 LEARLAEIEGG++CR+ PSGLAAIAMVN A LKSGDDVLLPDNVYGP++D WLERDF Sbjct: 70 VLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERDF 129 Query: 126 GITARFYDPLIGAGIAGLIQDNTRLIWTEAPGSVSMEVSDVPAICRAARARGVPVAMDNT 185 ITARFYDP+IG G+A LI+ TRL+WTEAPGSVSMEV D+PA+CRAA A+GV VA+DNT Sbjct: 130 QITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAKGVLVAIDNT 189 Query: 186 WSAGLAFRAFDHGVDIV-------XGGGR----GAVITCDDELNHKIELAHMRLGFGVGM 234 WSAG+AF+AF+HGVDIV GG GAVI D LN K+ELAHMRLGFGVGM Sbjct: 190 WSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNEKLELAHMRLGFGVGM 249 Query: 235 DDVYMVLRSLSSMRLRFDAHDAAARKVATWLKQRLEIIKVLHPALPDCPGHELWQRDFTG 294 DDVYMVLRSLSSMRLRF+AHDAAARKVA WLKQR EI+ VLHPA DCPGH W+RDF+G Sbjct: 250 DDVYMVLRSLSSMRLRFEAHDAAARKVAAWLKQRPEIVAVLHPAFEDCPGHAHWKRDFSG 309 Query: 295 AGGLFSVIFDEGFSEAQIDRFVDGLALFKIGFSWGGAHSLCVPYRMQTMRKSWTEKGGLV 354 AGGLFSVIFD + EAQ DRFVD L LFKIG+SWGGAHSLCVPYRM+ MR++W +G LV Sbjct: 310 AGGLFSVIFDPRYVEAQTDRFVDSLRLFKIGYSWGGAHSLCVPYRMKGMRRAWMREGQLV 369 Query: 355 RFNIGLEAAEDLIADIAQSLDAL 377 RFNIGLE+ EDLIADI Q+L L Sbjct: 370 RFNIGLESPEDLIADIEQALARL 392 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 112 bits (280), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 103/377 (27%), Positives = 159/377 (42%), Gaps = 54/377 (14%) Query: 52 SGYTYGLHGTPTTFTLEARLAEIEGGRHCRIVPSGLAAIAMVNLALLKSGDDVLLPDNVY 111 G Y PT LE RLA +EGG + SG AA+ + ++GD+++ +Y Sbjct: 54 QGNIYSRIMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLY 113 Query: 112 GPNRDQVVWLERDFGITARFYDPLIGAGIAGLIQDNTRLIWTEAPGSVSMEVSDVPAICR 171 G + +G+ RF DP A LI D T+ I+ E+ G+ ++D+ A+ Sbjct: 114 GGTYNLFAHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVAD 173 Query: 172 AARARGVPVAMDNTWSAGLAFRAFDHGVD--------------------IVXGGG----- 206 A GVP+ +DNT + R F+HG D IV G Sbjct: 174 IAHRHGVPLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAK 233 Query: 207 ---RGAVITCDDELNHKI-------ELAHM---------RLGFGVGMDDVYMVLRSLSSM 247 R + D H + E A++ +G + + + +L+ + ++ Sbjct: 234 HKQRFKRLNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETL 293 Query: 248 RLRFDAHDAAARKVATWLKQRLEIIKVLHPALPDCPGHELWQRDFTG-AGGLFSVIFDEG 306 LR D A VA +LK+ ++ V + L D P H L Q F G A G+ + G Sbjct: 294 GLRLDRITANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANG 353 Query: 307 FSEAQIDRFVDGLALFKIGFSWGGAHSLCVPYRMQTMRK---SWTEKGGL----VRFNIG 359 RF D L LF + G A SL T R+ + EK G+ VR +IG Sbjct: 354 REGGA--RFQDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIG 411 Query: 360 LEAAEDLIADIAQSLDA 376 +E +DL+AD+ Q+L + Sbjct: 412 IEHIDDLLADLEQALQS 428 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 98.6 bits (244), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 102/416 (24%), Positives = 169/416 (40%), Gaps = 49/416 (11%) Query: 3 EKYTPQTSLIHSKYAAPDNFGALPVAINHASTVLFPNVAAMRARNWKEKSGYTYGLHGTP 62 + Y T+++HS G+L I+ + T + + + ++ GY YG G P Sbjct: 5 KTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGRQGNP 64 Query: 63 TTFTLEARLAEIEGGRHCRIVPSGLAAIAMVNLALLKSGDDVLLPDNVYGPNRDQVVWLE 122 T LE ++ ++E G+ +G+AAI + LL+ GD V+ ++G N + + Sbjct: 65 TVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFG-NTNSLWMTV 123 Query: 123 RDFGITARFYDPLIGAGIAGLIQDNTRLIWTEAPGSVSMEVSDVPAICRAARARGVPVAM 182 G D + I NTRL++ E + +V+D+ I R RG+ + Sbjct: 124 GAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGILYVV 183 Query: 183 DNTWSAGLAFRAFDHGVDIVX----------GGGRGAVITCDDEL----------NHKIE 222 DNT ++ FR G +V G G +T E N+K Sbjct: 184 DNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAENYKKN 243 Query: 223 ------LAHMR------LGFGVGMDDVYMVLRSLSSMRLRFDAHDAAARKVATWLKQRLE 270 +A +R G +G + + + ++ LR + A +A L+ Sbjct: 244 PAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQADER 303 Query: 271 IIKVLHPALPDCPGHELWQRDFTGAGGLFSVIFDEGFSEAQIDRFVDGLALFKIGF---S 327 + V +P L P H L + F G L S +G ID F D L ++ + Sbjct: 304 VAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDG-----IDCF-DYLNRLRLAIPTSN 357 Query: 328 WGGAHSLCVP------YRMQTMRK-SWTEKGGLVRFNIGLEAAEDLIADIAQSLDA 376 G +L +P Y M R+ S L+R ++GLE +DL+AD Q+LDA Sbjct: 358 LGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVADFRQALDA 413 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,372,619 Number of extensions: 62267 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 4 Length of query: 381 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 294 Effective length of database: 1,207,925 Effective search space: 355129950 Effective search space used: 355129950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)