BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0024.0023.p
         (432 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312296|ref|YP_003776388.1| 2-methylthioadenine synthetase ...   800   0.0  
gi|300310058|ref|YP_003774150.1| tRNA modifying 2-methylthioaden...   142   6e-35

>gi|300312296|ref|YP_003776388.1| 2-methylthioadenine synthetase
           [Herbaspirillum seropedicae SmR1]
          Length = 458

 Score =  800 bits (2065), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 398/432 (92%), Positives = 417/432 (96%)

Query: 1   VDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGFIDAAVQESLDAIGEALSENGRVIVTG 60
           VDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGFIDAAVQESLDAIGEAL+ENG+VIVTG
Sbjct: 27  VDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGFIDAAVQESLDAIGEALNENGKVIVTG 86

Query: 61  CLGAKKDGDGDDLIQKIHPKVLEVTGPHAVGEVMQAVHKHLPKPHAPFLDLVPAQGVKLT 120
           CLGAKKD +GDD+IQKIHPKVLEVTGPHAVGEVMQAVHKHLPKPHAPF+DLVP QGVKLT
Sbjct: 87  CLGAKKDAEGDDIIQKIHPKVLEVTGPHAVGEVMQAVHKHLPKPHAPFIDLVPPQGVKLT 146

Query: 121 PKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIADIMLEAENLFNAGVKELLVISQDTS 180
           PKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIAD+MLEAENLF AGVKELLVISQDTS
Sbjct: 147 PKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIADVMLEAENLFKAGVKELLVISQDTS 206

Query: 181 AYGVDVKFRTGFWNGKPVKTHMTQLVEALGELASRYGAWVRLHYVYPYPHVDQIIPFMAQ 240
           AYGVDVKFRTGFWNGKPVKTHMTQLV ALGELA +YGAWVRLHYVYPYPHVDQIIP MA+
Sbjct: 207 AYGVDVKFRTGFWNGKPVKTHMTQLVGALGELAQQYGAWVRLHYVYPYPHVDQIIPMMAE 266

Query: 241 GHVLPYLDVPLQHAHPDVLKRMKRPASGEKNIERIQAWRKMCPDLTIRSTFIAGFPGETE 300
           G +LPYLDVPLQHAHPDVLKRMKRPA+GEKNIERIQAWR MCPDLTIRSTFIAGFPGETE
Sbjct: 267 GKILPYLDVPLQHAHPDVLKRMKRPANGEKNIERIQAWRAMCPDLTIRSTFIAGFPGETE 326

Query: 301 AEFEYLLDFLKEAEIDRLGCFAYSPVEGATANLLDNPVPEEVREERRGRVMQLQEEISKK 360
           AEFEYLLDFLKEAEIDRLGCFAYSPVEGATAN + NPVPEEVREERRGRVMQLQEEISKK
Sbjct: 327 AEFEYLLDFLKEAEIDRLGCFAYSPVEGATANEIANPVPEEVREERRGRVMQLQEEISKK 386

Query: 361 RLQAKVGKTLRVLIDEIDRSGGIGRSSADAPEIDGVVYVKPPFEPHKKLVVGEFVDVVVT 420
           RLQAKVGKTLRVLIDE+DR+GG GR+ +DAPEIDGVVYVKPPFEPH+KL VG+FVDV +T
Sbjct: 387 RLQAKVGKTLRVLIDEVDRNGGTGRTYSDAPEIDGVVYVKPPFEPHRKLEVGQFVDVRIT 446

Query: 421 AADAHDLWAAAQ 432
           AADAHDLW AA+
Sbjct: 447 AADAHDLWGAAE 458


>gi|300310058|ref|YP_003774150.1| tRNA modifying 2-methylthioadenine
           synthetase [Herbaspirillum seropedicae SmR1]
          Length = 446

 Score =  142 bits (357), Expect = 6e-35,   Method: Compositional matrix adjust.
 Identities = 120/441 (27%), Positives = 212/441 (48%), Gaps = 45/441 (10%)

Query: 2   DSEQILTQLRA-EGYDTAKSYDGADLVIVNTCGFIDAAVQESLDAIGE--ALSENGRVIV 58
           DS+++   L A +G       + AD++++NTC   + A ++    +G   AL +N   ++
Sbjct: 18  DSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVFSDLGRLRALKKNNPALL 77

Query: 59  TGCLGAKKDGDGDDLIQKIHPKVLEVTGPHAVGEVMQAV--HKHLPKPHA--------PF 108
            G  G     +G+ +I++  P V  V GP  +  + Q +   ++  +P           F
Sbjct: 78  IGVGGCVASQEGEAIIKR-APFVDMVFGPQTLHRLPQMISERRYSGRPQVDISFPEIEKF 136

Query: 109 LDLVPAQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIADIMLEAENLFNAG 168
             L PA+    T    AY+ I EGC+  CS+C++P  RG+ VSR   D++ E   L + G
Sbjct: 137 DHLPPARVEGAT----AYVSIMEGCSKYCSYCVVPYTRGEEVSRRFEDVLTEVAGLADQG 192

Query: 169 VKELLVISQDTSAYGVDVKFRTGFWNGKPVKTHMTQLVEALGELASRYGAWVRLHYVYPY 228
           VKE++++ Q+ +AY      R    +G+     +  L+E + EL        R+ +V  +
Sbjct: 193 VKEIMLLGQNVNAY------RGVMEDGEIADFAL--LIEYIAELP----GIERIRFVTSH 240

Query: 229 PH--VDQIIPFMAQ-GHVLPYLDVPLQHAHPDVLKRMKRPASGEKNIERIQAWRKMCPDL 285
           P     ++I   A+   +  +L +P QH     L  MKR  +  +    I+  RK+ PD+
Sbjct: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300

Query: 286 TIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATANLLDNPVPEEVREE 345
           T++S FI GFPGETE +FE L+  + +   D    F +S   G  A  L +  P+EV+  
Sbjct: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360

Query: 346 RRGRVMQLQEEISKKRLQAKVGKTLRVLIDEIDRSGG---IGRSSAD-APEIDGVVYVKP 401
           R  R+     + + +   A+VG   RVL++ + +       GR+ ++     DG      
Sbjct: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDG------ 414

Query: 402 PFEPHKKLVVGEFVDVVVTAA 422
              P+ + ++G+ ++V +T A
Sbjct: 415 --GPNGQRLIGQIIEVSITEA 433


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.138    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,235,722
Number of extensions: 49371
Number of successful extensions: 146
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 144
Number of HSP's successfully gapped: 2
Length of query: 432
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 344
Effective length of database: 1,203,190
Effective search space: 413897360
Effective search space used: 413897360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)