BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0022.p (185 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312301|ref|YP_003776393.1| polybetahydroxybutyrate accumul... 341 2e-95 >gi|300312301|ref|YP_003776393.1| polybetahydroxybutyrate accumulation regulatory protein PhbF [Herbaspirillum seropedicae SmR1] Length = 188 Score = 341 bits (874), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 162/177 (91%), Positives = 168/177 (94%) Query: 1 MTSAKKSGERLIKKYPNRRLYDTQTSSYITLTDVKQLVLDDEEFAVVDAKNGDDLTRSIL 60 MT+AK S +RLIKKYPNRRLYDTQTSSYITLTDVKQLVLD+EEF VVDAK+GDDLTRSIL Sbjct: 1 MTTAKNSADRLIKKYPNRRLYDTQTSSYITLTDVKQLVLDNEEFTVVDAKSGDDLTRSIL 60 Query: 61 LQIILEEESHGAPMFSSAALSQIIRYYGHAMQGMMGSYLEKNIQAFIDIQNKLTENSKGF 120 LQIILEEESHG PMFSS ALSQIIRYYGHAMQGMMG+YLEKNIQ FIDIQNKL ENSKGF Sbjct: 61 LQIILEEESHGVPMFSSGALSQIIRYYGHAMQGMMGNYLEKNIQTFIDIQNKLAENSKGF 120 Query: 121 YEGKPFSPEMWAQFMNVQGPMMQGMMSNYIEQSKTLFVQMQEQMQSQTKNMFGTFPF 177 YEGKPFSPEMW QFMNVQGPMMQGMMSNYIEQSK LF+QMQEQMQSQTKNMFGTFPF Sbjct: 121 YEGKPFSPEMWTQFMNVQGPMMQGMMSNYIEQSKNLFIQMQEQMQSQTKNMFGTFPF 177 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 509,482 Number of extensions: 17864 Number of successful extensions: 40 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 39 Number of HSP's successfully gapped: 1 Length of query: 185 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 105 Effective length of database: 1,241,070 Effective search space: 130312350 Effective search space used: 130312350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)