BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0021.p (246 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 468 e-133 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 182 2e-47 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 160 1e-40 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 144 4e-36 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 133 2e-32 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 468 bits (1204), Expect = e-133, Method: Compositional matrix adjust. Identities = 225/246 (91%), Positives = 236/246 (95%) Query: 1 MVKRIAYVTGGMGGIGTPICARLAKDGYTVVAGCGPNSARKDKWLASMREQGFDVHASEG 60 M KRIAYVTGGMGGIGT IC RL KDGYTVVAGCGPNS RKDKWLA+MREQGFD+HASEG Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 61 NVADWESTKAAFDKVRAEIGEVDVLVNNAGITRDGQFRKMSKSDWDAVIDTNLNSLFNVT 120 NV++WESTKAAF+KVRAEIGEVDVLVNNAGITRDGQFRKMSK DWDAVIDTNLNSLFNVT Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 121 KQVIEGMVDRNWGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 KQVIEGM +R +GRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 Query: 181 VSPGYVGTDMVRAIRPEVLEKIVAGIPVKRLAEPEEIASIIAWIASDEGGYATGSDFSLN 240 VSPGY+GTDMVRAIRPEVLEKIV+GIPVKRL EPEEIASI+AWIAS EGGYATGSDFSLN Sbjct: 181 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLN 240 Query: 241 GGLHMG 246 GGL+MG Sbjct: 241 GGLYMG 246 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 182 bits (462), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 99/246 (40%), Positives = 153/246 (62%), Gaps = 6/246 (2%) Query: 4 RIAYVTGGMGGIGTPICARLAKDGYTVVAGCGPNS--ARKDKWLASM-REQGFDVHASEG 60 ++A VTG GIG I LA+ G V+ S A ++LA++ E G + Sbjct: 9 QVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAG---RGAVL 65 Query: 61 NVADWESTKAAFDKVRAEIGEVDVLVNNAGITRDGQFRKMSKSDWDAVIDTNLNSLFNVT 120 NV D E++ A ++V+ + G + +LVNNAGIT+D +M +WD+VI TNL ++ ++ Sbjct: 66 NVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLS 125 Query: 121 KQVIEGMVDRNWGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 + V+ GM+ GRIINI+SV G+ G GQ NY+ AKAG+ G + ALA+E+ ++ +TVN Sbjct: 126 RAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNC 185 Query: 181 VSPGYVGTDMVRAIRPEVLEKIVAGIPVKRLAEPEEIASIIAWIASDEGGYATGSDFSLN 240 V+PG++ TDM RA+ + I+ IP+ RL EEIA +A++AS + Y TG+ ++N Sbjct: 186 VAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVN 245 Query: 241 GGLHMG 246 GG++MG Sbjct: 246 GGMYMG 251 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 160 bits (404), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 93/238 (39%), Positives = 133/238 (55%), Gaps = 4/238 (1%) Query: 7 YVTGGMGGIGTPICARLAKDGYTVVAGCGPNSARKDKWLASMREQGFDVHASEGNVADWE 66 +VTG GIG I RLA++GY +V C D+ A + G V + +VA+ E Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 Query: 67 STKAAFDKVRAEIGEVDVLVNNAGITRDGQFRKMSKSDWDAVIDTNLNSLFNVTKQVIEG 126 + G +V NAGITRD F M DWD+V+ TNL+ +NV ++ Sbjct: 70 QAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLVMP 129 Query: 127 MVDRNW-GRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGY 185 MV R GRI+ ++SV+G G GQ NYS AKAG+ G T ALA E+A + +TVN V+PG Sbjct: 130 MVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAPGL 189 Query: 186 VGTDMVRAIRPEVLEKIVAGIPVKRLAEPEEIASIIAWIASDEGGYATGSDFSLNGGL 243 + TDM +++I+ IP +R +PEE+A+ +A++ S + Y T S+NGGL Sbjct: 190 IDTDMT---EDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 144 bits (364), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 89/247 (36%), Positives = 134/247 (54%), Gaps = 8/247 (3%) Query: 4 RIAYVTGGMGGIGTPICARLAKDGYTV-VAGCGPNSARK--DKWLASMREQGFDVHASEG 60 R+A VTGG GIG I +LA G+TV VA ++A K D +A+ G + Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAA----GHRAAPLQM 64 Query: 61 NVADWESTKAAFDKVRAEIGEVDVLVNNAGITRDGQFRKMSKSDWDAVIDTNLNSLFNVT 120 ++ ES AAF + + G DVLVNNAG+ R F +W ++ N+ + Sbjct: 65 DLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAG 124 Query: 121 KQVIEGMVDRNWGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 + MV + WGRI+NISS++G + G+T Y T+KA + G T +A E+A G+TVN+ Sbjct: 125 QHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNS 184 Query: 181 VSPGYVGTDMVRAIRPE-VLEKIVAGIPVKRLAEPEEIASIIAWIASDEGGYATGSDFSL 239 V+PG V T M +A+ E E +P++R +EIAS + ++A++E Y TG + Sbjct: 185 VAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPV 244 Query: 240 NGGLHMG 246 +GG G Sbjct: 245 DGGFVAG 251 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 133 bits (334), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 78/242 (32%), Positives = 126/242 (52%), Gaps = 3/242 (1%) Query: 4 RIAYVTGGMGGIGTPICARLAKDGYTVVAGCGPNSARKDKWLASMREQGFDVHASEGNVA 63 ++A +TG GGIG + +A+ G +V G ++ + A +R +G +H S + Sbjct: 20 KLALITGSSGGIGLALARGMAQAGAAIVLN-GRDAGKLAAVAAQLRAEGHTIHESSFDAT 78 Query: 64 DWESTKAAFDKVRAEIGEVDVLVNNAGITRDGQFRKMSKSDWDAVIDTNLNSLFNVTKQV 123 E KAA K+ +EIG + +LVNNAGI R + +SDW +I TNLNS+F + V Sbjct: 79 SSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQAV 138 Query: 124 IEGMVDRNWGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSP 183 M+ R G+IINI SV + G+ G Y+ K + FT +A + G+ VN + P Sbjct: 139 ARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGP 198 Query: 184 GYVGTDMVRAI--RPEVLEKIVAGIPVKRLAEPEEIASIIAWIASDEGGYATGSDFSLNG 241 GY T+M A+ + + +V P +R + E++ ++ASD + G ++G Sbjct: 199 GYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDG 258 Query: 242 GL 243 G+ Sbjct: 259 GV 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 749,379 Number of extensions: 28527 Number of successful extensions: 222 Number of sequences better than 1.0e-08: 50 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 50 Length of query: 246 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 163 Effective length of database: 1,226,865 Effective search space: 199978995 Effective search space used: 199978995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)