BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0020.p (565 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthe... 781 0.0 gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synt... 299 4e-82 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 69 1e-12 >gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthetase [Herbaspirillum seropedicae SmR1] Length = 588 Score = 781 bits (2016), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/464 (79%), Positives = 416/464 (89%) Query: 6 KQLGDLGATLDPADVVRLQQDYADQFASLWQTAVNSQIPAVTDRRMSAPAWQSNPFFAFH 65 KQL D GAT+DPA++VRLQQDY QF +LWQ+ + ++ PA+TDRR+SAPAWQ +P+FAF Sbjct: 50 KQLADNGATIDPAELVRLQQDYTQQFGALWQSMLAAKTPAITDRRLSAPAWQEHPYFAFQ 109 Query: 66 AAAYQLNARFLTSMVDAVQSTDKVKQKLRFAVQQMIDALSPANFLATNPEAQQKIIDTHG 125 AAAYQLN+RFL ++ DAVQ ++KV+ KLRFAVQQMIDA+SPANFLATNP AQQKI++T G Sbjct: 110 AAAYQLNSRFLLALADAVQGSEKVRHKLRFAVQQMIDAMSPANFLATNPVAQQKILETRG 169 Query: 126 ESLTRGIAQLFADLQKGRVSQTDETAFEVGRDVATTAGSVVFENELFQLIQYAPLTKTVH 185 ESLT+GIAQL ADLQKG VSQTDETAFEVGRDV + GSV+FENELFQLIQY PLTKTVH Sbjct: 170 ESLTKGIAQLLADLQKGHVSQTDETAFEVGRDVGASEGSVIFENELFQLIQYKPLTKTVH 229 Query: 186 ARPLLIVPPCINKFYILDLQPQNSLVRYAVEQGNTVFLISWRNADASMETATWDQYVDDA 245 PLLIVPPCINKFYILDLQP NSLVRY VEQG+TVFLISWRNADA++ETATWDQYVDDA Sbjct: 230 EVPLLIVPPCINKFYILDLQPHNSLVRYTVEQGHTVFLISWRNADAAVETATWDQYVDDA 289 Query: 246 VVNAIHVAQEISGQDKINALGFCVGGTILSCALAVLAARGEEPVASLTLLTAFLNFLDTG 305 VV +IH+ QEIS Q +INALGFCVGGTILSCALA+LAARGE+PVASLTLLT FL+F DTG Sbjct: 290 VVRSIHIVQEISKQPQINALGFCVGGTILSCALALLAARGEDPVASLTLLTTFLDFSDTG 349 Query: 306 AIDVYIDDVQIGMREQMIGKSGLMSGRDFASAFSSLRPNDLLWNYVESNYLKGETPQAFD 365 AIDVYID++Q+ +REQMIG+ GLM+GRDFASAFSSLRPNDLLWNYVESNYLKGE P AFD Sbjct: 350 AIDVYIDEMQLALREQMIGQRGLMAGRDFASAFSSLRPNDLLWNYVESNYLKGEKPSAFD 409 Query: 366 LLYWNADSTNLPGPMFCYYLRHMYLENALKEPGKLTIAGEKIDLYKLNMPAFIYASREDH 425 LLYWNADSTNLPGPMFCYYLRHMYLENALKEPG+LT+ GEKIDL K+N PAFIYASR+DH Sbjct: 410 LLYWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTVGGEKIDLRKINAPAFIYASRDDH 469 Query: 426 IVPWASAYASTAILNAKKPANNRFVLGASGHIAGVINPPDXNRR 469 IVPW SAYAS ++N K +NNRFVLGASGHIAGVINP N+R Sbjct: 470 IVPWKSAYASLGLINTAKRSNNRFVLGASGHIAGVINPASKNKR 513 >gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synthase [Herbaspirillum seropedicae SmR1] Length = 590 Score = 299 bits (765), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 166/442 (37%), Positives = 249/442 (56%), Gaps = 12/442 (2%) Query: 46 VTDRRMSAPAWQSNPFFAFHAAAYQLNARFLTSMVDAVQSTDKVKQKL-RFAVQQMIDAL 104 V DRR S P WQ PF +H + + L ++ + V +K L FA +Q +D Sbjct: 100 VADRRFSNPDWQRWPFNLWHQS-FLLAEQWWAAATRDVWGVEKHHADLVAFAARQCLDVF 158 Query: 105 SPANFLATNPEAQQKIIDTHGESLTRGIAQLFADLQKGRVSQTDETA--FEVGRDVATTA 162 SP+N L TNPE + ++ G +L G+ D+ + + TA F GRDVA + Sbjct: 159 SPSNQLLTNPEVLRITMEQRGANLLMGMQNCMNDMIGLLLEKPASTARTFMPGRDVAVSP 218 Query: 163 GSVVFENELFQLIQYAPLTKTVHARPLLIVPPCINKFYILDLQPQNSLVRYAVEQGNTVF 222 G VV N + +LI+YAP T+TV PLL++P I K+YILDL P NSL+RY VE+G+TVF Sbjct: 219 GKVVLRNRIMELIRYAPATETVCPEPLLLIPAWIMKYYILDLSPANSLIRYLVEKGHTVF 278 Query: 223 LISWRNADASMETATWDQYVDDAVVNAIHVAQEISGQDKINALGFCVGGTILSCALAVLA 282 +SW+N + + D Y+D A+ K++ +G+C+GGT+LS A A +A Sbjct: 279 CVSWKNPAPADRDLSMDDYLDSGFHAALDAVNSACPDRKVHGVGYCLGGTLLSIAAAAMA 338 Query: 283 ARGEEPVASLTLLTAFLNFLDTGAIDVYIDDVQIGMREQMIGKSGLMSGRDFASAFSSLR 342 G++ +ASLTLL A +F + G + ++ID+ QI + E + ++G +S A+AF LR Sbjct: 339 RDGDQRLASLTLLAAQTDFTEPGELGLFIDESQISLLEAQMQQAGTLSQSQMAAAFQMLR 398 Query: 343 PNDLLWNYVESNYLKGETPQAFDLLYWNADSTNLPGPMFCYYLRHMYLENALKEPGKLTI 402 DLLW+ V YL G DL+ WNAD+T +P M YLR ++L++ L E G+ + Sbjct: 399 SYDLLWSRVIRQYLMGVPAHNSDLMEWNADATRMPALMHSQYLRRLFLDDDLSE-GRYEV 457 Query: 403 AGEKIDLYKLNMPAFIYASREDHIVPWASAYASTAILNAKKPANNRFVLGASGHIAGVIN 462 G+ + L +N+P F+ + DH+ PW S Y L++ PA FVL + GH AG+++ Sbjct: 458 DGKPVLLADVNLPVFLVGTVRDHVAPWRSVYK----LHSLCPAEITFVLASGGHNAGIVS 513 Query: 463 PPDXNRRKRNAAT---GPTTRS 481 PP R + +T G + RS Sbjct: 514 PPGHPRSQFQISTRAAGASYRS 535 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 68.6 bits (166), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 69/252 (27%), Positives = 112/252 (44%), Gaps = 29/252 (11%) Query: 188 PLLIVPPCINKFYIL-DLQPQNSLVRYAVEQG-NTVFLISWRNADASMETATWDQYVDDA 245 P LIV P ++ D SLV + G + V LI W +A A M+ D Y+ D Sbjct: 87 PTLIVAPYAGHTSVIADYDQGQSLVETLLGNGVDHVLLIDWHSASADMKDLEIDNYLADL 146 Query: 246 VVNAIHVAQEISGQDKINALGFCVGGTILSCALAVLAARGEEPVASLTLLTAFLNF-LDT 304 V V E+ G ++ +G C GG I A++AAR E VA L L + ++ Sbjct: 147 CV----VIDELGG--RVRLVGLCQGGWI----SAMIAARFPEKVARLVLAGSPIDAGAGE 196 Query: 305 GAIDVYIDDVQIGMREQMI-GKSGLMSGRDFASAFSSLRPN--------DLLWNYVESNY 355 G + + + + E ++ GLM G + ++ P+ +LL + + Y Sbjct: 197 GPLKQMVQRLPLRFYEDLVRSGGGLMRGSFMLQGWKNMHPDEHYFNEHLELLQHIDDPAY 256 Query: 356 L-KGETPQAFDLLYWNADSTNLPGPMFCYYLRHMYLENALKEPGKLTIAGEKIDLYKLNM 414 L K ET A W + +LPG + + H++ +N L GK G+++DL ++ Sbjct: 257 LAKRETFAA-----WYETTVDLPGRWYLQAIGHIFKDNLLAR-GKYVALGQQLDLRQITC 310 Query: 415 PAFIYASREDHI 426 P ++ A D I Sbjct: 311 PLYLLAGERDDI 322 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,758,063 Number of extensions: 74418 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 3 Length of query: 565 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 475 Effective length of database: 1,193,720 Effective search space: 567017000 Effective search space used: 567017000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)