BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0013.p (114 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312307|ref|YP_003776399.1| ribosomal large subunit pseudou... 140 3e-35 gi|300311211|ref|YP_003775303.1| ribosomal large subunit pseudou... 67 5e-13 >gi|300312307|ref|YP_003776399.1| ribosomal large subunit pseudouridine synthase D [Herbaspirillum seropedicae SmR1] Length = 370 Score = 140 bits (353), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 73/105 (69%), Positives = 84/105 (80%) Query: 1 MTAKPAVTHYRRAAVGALDGRAVSLMRCQLETGRTHQIRVHMQSLGFALVGDALYGKSHL 60 M AKPAVTHYRR A G +D + VSL+ C+LETGRTHQIRVH+QSLGF LVGDALYGK HL Sbjct: 240 MRAKPAVTHYRRLATGEIDRKPVSLIHCRLETGRTHQIRVHLQSLGFPLVGDALYGKHHL 299 Query: 61 MPFFPRQALQAWKLGLIHPATGKQVEWEVGLPEDFTELLERAGID 105 + FPRQAL A +LGL+HP+TGK +W LPED LL+RAGID Sbjct: 300 VSVFPRQALLAGRLGLLHPSTGKFRQWAAPLPEDIVALLQRAGID 344 >gi|300311211|ref|YP_003775303.1| ribosomal large subunit pseudouridine synthase C [Herbaspirillum seropedicae SmR1] Length = 355 Score = 66.6 bits (161), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 34/86 (39%), Positives = 46/86 (53%), Gaps = 10/86 (11%) Query: 24 SLMRCQLETGRTHQIRVHMQSLGFALVGDALYGKSHLMP----------FFPRQALQAWK 73 +L+ +L+TGRTHQIRVH+ S GF +VGD YG L F R L AW+ Sbjct: 248 ALLEAELKTGRTHQIRVHLSSSGFPIVGDDKYGDFALNKALQKADGRRIAFKRMFLHAWR 307 Query: 74 LGLIHPATGKQVEWEVGLPEDFTELL 99 + HP +G V + GLP++ L Sbjct: 308 ISFRHPESGDTVTLKAGLPQECASFL 333 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 400,542 Number of extensions: 16403 Number of successful extensions: 41 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 40 Number of HSP's successfully gapped: 2 Length of query: 114 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 41 Effective length of database: 1,274,215 Effective search space: 52242815 Effective search space used: 52242815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 124 (52.4 bits)