BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0006.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 688 0.0 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 115 5e-27 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 114 2e-26 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 83 3e-17 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 70 3e-13 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 688 bits (1776), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/386 (84%), Positives = 356/386 (92%), Gaps = 1/386 (0%) Query: 1 MSQPANIVSKLPRVGTTIFTVMSALASEKGAVNLGQGFPDFDCDPALVDAVTSAMKTGLN 60 MS P NIV+KLP+VGTTIFTVMSALASEKGAVNLGQGFPDF CDPALV AVT AM+ GLN Sbjct: 1 MSTP-NIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVAAVTQAMQEGLN 59 Query: 61 QYPPMAGVPLLREAIAQKIKTLHGHTYDALNEITVTAGATQGILTSILCAVHAGDEVIVI 120 QYPPMAGVP+LREAIA+K++ L+GH YD ++EITVTAGATQGILTS+LCAVH GDEVIVI Sbjct: 60 QYPPMAGVPVLREAIAEKVEKLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVI 119 Query: 121 EPAYDCYVPAIELAGGVPVFVQMTVSSDGYSVPWDKVAAAVTSKTRLIMVNTPHNPTGSI 180 EP YDCYVPAIELAGGVPVFVQM V + GYS+PWDKV AAVT KTR+IMVNTPHNPTGS+ Sbjct: 120 EPVYDCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSV 179 Query: 181 LKKTDINALADIVRGTDILILSDEVYEHMVYDGQAHESLCRHPELAPRTFINSSFGKTYH 240 ++ D+ ALADIVRGTDILILSDEVYEHMVYDGQ HESL RHPELA R+FINSSFGKTYH Sbjct: 180 MRAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYH 239 Query: 241 VTGWKIGFVAAPAALTAEFRKVHQFNVFTVNTPVQYGIAEYMKNPAPYVELPAFYQRKRD 300 VTGWK+G+VAAPAALTAEFRKVHQFNVFTVNTPVQYG+A YMK+PAPY++LPAFYQ KRD Sbjct: 240 VTGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRD 299 Query: 301 LFRDGLKNTRFKLLPSDGTYFQCVEYGAISDLSEAEFSKWLTTEIGVAAIPVSAFYNTPK 360 LFR GL NTRF+LLPS GTYFQCV+YGAIS L EAEF KWLT+EIGVAAIPVSAFYNTP+ Sbjct: 300 LFRTGLANTRFELLPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVAAIPVSAFYNTPR 359 Query: 361 ESGIVRFCFAKKDETLRLALDRLAKL 386 ESGIVRFCFAKKDETL+LAL+RLAKL Sbjct: 360 ESGIVRFCFAKKDETLQLALERLAKL 385 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 115 bits (289), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 101/363 (27%), Positives = 175/363 (48%), Gaps = 27/363 (7%) Query: 32 VNLGQGFPDFDCDPALVDAVTSAMKTGLNQYPPMAGVPLLREAIAQKIKTLHGHTYDALN 91 +++G G PDF PA+V+A T AM G QY G+P LR AI+ ++++G A Sbjct: 36 IHMGIGEPDFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAISDHYRSVYGLEI-APE 94 Query: 92 EITVTAGATQGILTSILCAVHAGDEVIVIEPAYDC---YVPAIE-----LAGGVPVFVQM 143 I VTAGA+ +L + V EV++ +P+Y C +V A E +A G Q+ Sbjct: 95 RIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQL 154 Query: 144 TVSSDGYSVPWDKVAAAVTSKTRLIMVNTPHNPTGSILKKTDINALADIVRGTDILILSD 203 S+ W S TR +++ +P NPTG+ + ++ A+ VR + D Sbjct: 155 --SAQMVREHWG-------SATRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTIVD 205 Query: 204 EVYEHMVYDGQAHESLCRHPELAPRTFINSSFGKTYHVTGWKIGFVAAPAALTAEFRKVH 263 E+Y+ + Y+G +L L + +SF K +++TGW++G++ P AL + K+ Sbjct: 206 EIYQGLSYEGAPFSALS----LGDDVVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLA 261 Query: 264 QFNVFTVNTPVQYGIAEYM--KNPAPYVELPAFYQRKRDLFRDGLKNTRFKL-LPSDGTY 320 Q + ++ Q+ + A Y E A ++R+RD L++ F + + DG + Sbjct: 262 QNLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRDYIVPALESLGFTVPVMPDGAF 321 Query: 321 FQCVEYGAISDLSEAEFSKWLTTEIGVAAIPVSAFYNTPKESGIVRFCFAKKDETLRLAL 380 + + A++D ++ + S + E GV +P F +R +A E L+ A+ Sbjct: 322 YVYADCSALADDAD-QLSLDMLNEAGVVLVPGLDF-GPFTARRYIRLSYATSMENLQEAV 379 Query: 381 DRL 383 RL Sbjct: 380 ARL 382 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 95/385 (24%), Positives = 174/385 (45%), Gaps = 23/385 (5%) Query: 10 KLPRVGTTIFTVMSAL---ASEKGA--VNLGQGFPDFDCDPALVDA-VTSAMKTGLNQYP 63 ++ R+ +F + + L A +G +++ G PD P +V+ V A + + Y Sbjct: 43 RIERLPPYVFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYS 102 Query: 64 PMAGVPLLREAIAQKIKTLHGHTYDALNEITVTAGATQGILTSILCAVHAGDEVIVIEPA 123 G+P LR AIA ++ + +D +E VT G+ +G+ +L + GD V+V P+ Sbjct: 103 SSKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPS 162 Query: 124 YDCYVPAIELAGGVPVFVQMTVSSDGYSVPWDKVAAAVTS---KTRLIMVNTPHNPTGSI 180 Y ++ +AG V+M+ D + D++ AV K +++++ P NPT Sbjct: 163 YPIHIYGAVIAGANIRSVRMSPGVDFF----DELERAVRESYPKPKMMILGFPSNPTAQC 218 Query: 181 LKKTDINALADIVRGTDILILSDEVYEHMVYDGQAHESLCRHP---ELAPRTFINSSFGK 237 ++ + + R IL++ D Y + +DG S+ + P E+A F + K Sbjct: 219 VELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAVEFF---TLSK 275 Query: 238 TYHVTGWKIGFVAAPAALTAEFRKVHQFNVFTVNTPVQY-GIAEYMKNPAPYVELPAFYQ 296 +Y++ GW+IGF+ A L A ++ ++ + TPVQ IA + + E+ A Y+ Sbjct: 276 SYNMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYE 335 Query: 297 RKRDLFRDGLKNTRFKL-LPSDGTYFQCVEYGAISDLSEAEFSKWLTTEIGVAAIPVSAF 355 R+R++ GL + + +P Y EF++ L + V P F Sbjct: 336 RRRNVLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGF 395 Query: 356 YNTPKESGIVRFCFAKKDETLRLAL 380 E VRF + + +R A+ Sbjct: 396 GEYGDE--YVRFALIENESRIRQAI 418 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 83.2 bits (204), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 57/206 (27%), Positives = 98/206 (47%), Gaps = 14/206 (6%) Query: 80 KTLHGHTYDALNEITVTAGATQGILTSILCAVHAGDEVIVIEPAYDCYVPAIELAGGVPV 139 K + G T D +I + GA++ I+ S+ ++ GDEV+V P Y + A+ L+GG PV Sbjct: 88 KKIEGVTID---DIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPV 144 Query: 140 FVQMTVSSDGYSVPWDKVAAAVTSKTRLIMVNTPHNPTGSILKKTDINALADIVRGTDIL 199 + G+ + +T T+ I+V P+NPTG++ + + ++ R ++ Sbjct: 145 HY-VCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLI 203 Query: 200 ILSDEVYEHMVYDGQAHESLCRHPELAPRT-FIN-SSFGKTYHVTGWKIGFVAAPAALTA 257 IL+DE+Y+ ++YDG H SL LA FI + K Y G++ G++ Sbjct: 204 ILADEIYDKVLYDGNTHTSLA---SLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKH 260 Query: 258 EFRKVHQFNVFT-----VNTPVQYGI 278 + N+ N P Q+ I Sbjct: 261 ARDYIEGLNMLASMRLCANAPGQFAI 286 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 70.1 bits (170), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 90/351 (25%), Positives = 140/351 (39%), Gaps = 53/351 (15%) Query: 32 VNLGQGFPDFDCDPALVDAVTSAMKTGLNQYPPMAGVPLLREAIAQKIKTLHGHTY-DAL 90 ++LG G P PA + GL YP G LR AIA ++ + D Sbjct: 49 ISLGIGEPKHPT-PAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRYDIPRPDPA 107 Query: 91 NEITVTAGATQGILT---SILCAVHAGDEVIVIEPAYDCYVPAIELAGGVPVFV----QM 143 EI G+ + + + +++ G V+ P Y Y + LAG P FV + Sbjct: 108 TEILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPKR 167 Query: 144 TVSSDGYSVPWDKVAAAVTSKTRLIMVNTPHNPTGSILKKTDINALADIVRGTDILILSD 203 + D VP D V + +L+ V +P NPTG++L D L + +I SD Sbjct: 168 NFAPDFSQVPQD-----VWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASD 222 Query: 204 EVYEHMVYDG-------QAHESLCRHPELAPRTFINSSFGKTYHVTGWKIGFVAAPAALT 256 E Y + ++ QA L R R SS K +V G + GFVA AA+ Sbjct: 223 ECYSEIYFNEDPPLGGMQAARLLGRDYR---RLISFSSLSKRSNVPGMRSGFVAGDAAIL 279 Query: 257 AEF---RKVHQFNVFTV-----------NTPVQYGIAEYMKNPAPYVELPAFYQRKRDLF 302 +F R H + V T VQ IA+Y+ + + L Sbjct: 280 KKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQGNIAKYVTK----------FSQVTPLL 329 Query: 303 RDGLKNTRFKLLPSDGTYFQCVEYGAISDLSEAEFSKWLTTEIGVAAIPVS 353 ++ L+ LP D ++ + + ++S+ EF++ L E V +P S Sbjct: 330 QEVLEVA----LP-DAAFYLWAKVDKLVNISDTEFAQRLYAEYNVTVLPGS 375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,212,838 Number of extensions: 50382 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 5 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)