BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0005.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 86 2e-18 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 81 6e-17 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 77 1e-15 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 74 1e-14 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 66 2e-12 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 86.3 bits (212), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 61/201 (30%), Positives = 98/201 (48%), Gaps = 8/201 (3%) Query: 5 LKASRNDATLILTFANPGQRNAVDSNVLAAAIETLSKAERDDSVRALVLTGAERDFCGGV 64 L A + L LT P RNA+ + L + L++AE DDSVR +V++G E+ F G Sbjct: 11 LAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAFAAGA 70 Query: 65 E----VGKD-LATQIAALEALQNLIDMLRSFPKPVIAAVEGVALDAGFSLALGCDLIVAG 119 + + KD +ATQ L+ + FPKP++AAV G AL AG L + D+ +A Sbjct: 71 DLNEMIHKDAIATQ---LDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAA 127 Query: 120 QNAAFGISPSQVGTWAVGGAGWFLPKSLPPQWVAEILLDAAPLPAARLHGSGIINRLTSD 179 + A G VGT G L +++ +++L + A + +G++ + D Sbjct: 128 RGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDD 187 Query: 180 GDALDKALAWGEQLATTTPAA 200 L++ LA +A +P A Sbjct: 188 DATLERTLALAHSIAQKSPLA 208 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 81.3 bits (199), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 63/228 (27%), Positives = 102/228 (44%), Gaps = 12/228 (5%) Query: 10 NDATLILTFANPGQRNAVDSNVLAAAIETLSKAERDDSVRALVLTGAERDFCGGVEVGK- 68 D ++T P + N+ + + ++ + D SVR L+LTGA R FC G ++G Sbjct: 11 RDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAGQDLGDR 70 Query: 69 ---------DLATQIAALEALQNLIDMLRSFPKPVIAAVEGVALDAGFSLALGCDLIVAG 119 DL I + L+ LR+ P PVI AV GVA AG +L L CD+++A Sbjct: 71 AVKPGDDGVDLGESID--KYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIVLAA 128 Query: 120 QNAAFGISPSQVGTWAVGGAGWFLPKSLPPQWVAEILLDAAPLPAARLHGSGIINRLTSD 179 ++A+F ++G G +FLP+ + + L + A + G+I + D Sbjct: 129 RSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKCVDD 188 Query: 180 GDALDKALAWGEQLATTTPAAFEQFKTLLASASSTTLTEYFSLERHAL 227 +++A A A K L ++ + TL E LER + Sbjct: 189 DQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTM 236 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 76.6 bits (187), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 52/184 (28%), Positives = 87/184 (47%), Gaps = 1/184 (0%) Query: 1 MTAELKASRNDATLILTFANPGQRNAVDSNVLAAAIETLSKAERDDSVRALVLTGAERDF 60 M +++ S+ D L + F ++NA+ + + + L +AE D +VRA++ TG + F Sbjct: 1 MQMDIQLSKADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTGKPKAF 60 Query: 61 CGGVEVGKDLATQIAALEA-LQNLIDMLRSFPKPVIAAVEGVALDAGFSLALGCDLIVAG 119 G ++ L +A + + + KP++AAV GVA+ G +L L CDL+ A Sbjct: 61 SAGNDLEDFLKNPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAA 120 Query: 120 QNAAFGISPSQVGTWAVGGAGWFLPKSLPPQWVAEILLDAAPLPAARLHGSGIINRLTSD 179 NA F + +Q+G + LP Q AE LL A G +NR+ + Sbjct: 121 DNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRVMAQ 180 Query: 180 GDAL 183 + L Sbjct: 181 EELL 184 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 73.6 bits (179), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 58/176 (32%), Positives = 87/176 (49%), Gaps = 7/176 (3%) Query: 16 LTFANPGQRNAVDSNVLAAAIETLSKAERDDSVRALVLTGAERDFCGGVEVGKDLAT-QI 74 LT P NA+ +L A L + D +VR ++L + FC G ++ + AT Q Sbjct: 20 LTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFCAGHDLRQMQATPQQ 79 Query: 75 AALEAL----QNLIDMLRSFPKPVIAAVEGVALDAGFSLALGCDLIVAGQNAAFGISPSQ 130 EAL +L+ LR P PVIA V+G+A AG L CDL +A ++A F +S Sbjct: 80 TYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATCDLAIAAESARFAVSGIN 139 Query: 131 VGTWAVGGAGWFLPKSLPPQWVAEILLDAAPLPAARLHGSGIINRLTSDGDALDKA 186 VG + A L ++LP + E+L+ + AA G+IN+ + + LD A Sbjct: 140 VGLFCSTPA-VALTRNLPIKRAFEMLVTGKFIDAATAAAWGLINQAVPEQE-LDSA 193 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 66.2 bits (160), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 57/218 (26%), Positives = 93/218 (42%), Gaps = 13/218 (5%) Query: 15 ILTFANPGQRNAVDSNVLAAAIETLSKAERDDSVRALVLTGAERDFCGGVEV----GKDL 70 ++ P NA+ ++ E L + + + +++TG+E+ F G ++ G D Sbjct: 16 LIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAGADISAMAGYDY 75 Query: 71 ATQIAALEALQNLIDMLRSFPKPVIAAVEGVALDAGFSLALGCDLIVAGQNAAFGISPSQ 130 + E + + LR KPVIAAV G AL G LA+ CD I+A NA FG + Sbjct: 76 -MDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIAADNAKFGQPEIK 134 Query: 131 VGTWAVGGAGWFLPKSLPPQWVAEILLDAAPLPAARLHGSGIINRLTSDGDALDKALAWG 190 +G G LP+++ ++ L + A +G+++R+ A DK L Sbjct: 135 LGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIV----AADKLLEEA 190 Query: 191 EQLATTTPAAFEQFKTLLAS----ASSTTLTEYFSLER 224 A Q ++ A TTL+E ER Sbjct: 191 MAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYER 228 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 685,812 Number of extensions: 26386 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 8 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)