BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0004.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 402 e-113 gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 211 6e-56 gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 65 5e-12 gi|300311017|ref|YP_003775109.1| glutathione S-transferase famil... 64 1e-11 gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herb... 56 2e-09 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 402 bits (1032), Expect = e-113, Method: Compositional matrix adjust. Identities = 188/230 (81%), Positives = 208/230 (90%) Query: 1 MIDVYSWATPNGHKVHIMLEELGLDYRAHAIDIGAGDQFADAFLKISPNNKIPAIVDSDG 60 MIDVYSWATPNGHKVHIMLEE GL Y AHAIDIGAGDQF FLKISPNNKIPAIVD DG Sbjct: 1 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDG 60 Query: 61 PDGEPISLFESGAILIYLAGKTGKLLGNTDRERFNVLQWVMFQMGGLGPMLGQAHHFRIY 120 PDGEPISLFESGAILIYLAGKTG+ LG T RE++ VLQWVMFQMGGLGPMLGQAHHFR+Y Sbjct: 61 PDGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLY 120 Query: 121 APEKIEYAVNRYSNEAKRLYGVLDKQLSKTAYLAGDEYTIADIASFPWTRSWKNQGIELS 180 APE+IEYAVNRY+NEA+RLY V+DKQL + AYLAG+ YTIADIA FPWTRS KNQGI+L Sbjct: 121 APEQIEYAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLD 180 Query: 181 EYPNVQRWFDAINERPAVQRGVTVLADKRKALTDDKARDILFGNSQYKKR 230 ++PNV+RW+DAI+ RPAVQRGVTVLADKRK L DDKA+++LFG SQY+KR Sbjct: 181 DFPNVKRWYDAISARPAVQRGVTVLADKRKPLHDDKAKELLFGKSQYQKR 230 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 211 bits (536), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 106/212 (50%), Positives = 141/212 (66%), Gaps = 7/212 (3%) Query: 2 IDVYSWATPNGHKVHIMLEELGLDYRAHAIDIGAGDQFADAFLKISPNNKIPAIVDSDGP 61 I +YS TPNG KV IMLEE GL Y AH + DQ + FL ++PNNKIPAI+D +GP Sbjct: 22 IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNGP 81 Query: 62 DGEPISLFESGAILIYLAGKTGKLLGNTDRERFNVLQWVMFQMGGLGPMLGQAHHFRIYA 121 G+P+ LFESGAIL+YLA KTG+ L R+ V+QWVMFQMGG+GPM GQ F +A Sbjct: 82 GGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWVMFQMGGVGPMFGQLGFFHRFA 141 Query: 122 PEKIE--YAVNRYSNEAKRLYGVLDKQLSKTAYLAGDEYTIADIASFPWTRSW-----KN 174 ++ E +RY NE+KRL GV+DK+L+++ +L GD Y+IADIA+FPW R+ Sbjct: 142 GKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWIRNLIGFYEAA 201 Query: 175 QGIELSEYPNVQRWFDAINERPAVQRGVTVLA 206 + ++P+V R +A RPAV RG+ + A Sbjct: 202 DLVGFQDFPHVARALEAFVARPAVVRGLNIPA 233 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 65.1 bits (157), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 51/190 (26%), Positives = 82/190 (43%), Gaps = 15/190 (7%) Query: 11 NGHKVHIMLEELGLDYRAHAIDIGAGDQFADAFLKISPNNKIPAIVDSDGPDGEPISLFE 70 + H+ + LGL +D+ AG AFL ++P ++P + D + + + + Sbjct: 11 HAHRAVLFASLLGLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGE------VVIAD 64 Query: 71 SGAILIYLAGKTGKL--LGNTDRERFNVLQWVMFQMGGL--GPMLGQAHHFRIYAPEKIE 126 S AIL+YLA K G+ L + V +W+ G L GP R+ Sbjct: 65 SNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAA-----RLVTVFGAA 119 Query: 127 YAVNRYSNEAKRLYGVLDKQLSKTAYLAGDEYTIADIASFPWTRSWKNQGIELSEYPNVQ 186 + A L G L++ L+K +LA D TIAD+A + + ++L YP VQ Sbjct: 120 FNAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQ 179 Query: 187 RWFDAINERP 196 + I P Sbjct: 180 GFLQRIEALP 189 >gi|300311017|ref|YP_003775109.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 60/232 (25%), Positives = 94/232 (40%), Gaps = 49/232 (21%) Query: 1 MIDVYSWATPNGHKVHIMLEELGLDYRAHAIDIGAGDQFADA-FLKISPNNKIPAIVDSD 59 MI V+ +V +LEELGLDY A LK+ P K P I D + Sbjct: 1 MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGE 60 Query: 60 GPDGEPISLFESGAILIYL----AGKTGKLLGNTDRERFNVLQWVMFQMGGLGP------ 109 +++ ESG I+ YL +G L + ER W+ F G P Sbjct: 61 ------LTVAESGNIIEYLLERYGAHSGLLPASGTPERLRYRYWLHFAEGSAMPWLVMQL 114 Query: 110 ------------------MLGQAHHFRIYAPEKIEYAVNRYSNEAKRLYGVLDKQLSKTA 151 ML + R+ AP++ +R ++ +L + Sbjct: 115 IFAKMPKAVPTLIRPIARMLAEGMRSRVVAPQQ------------ERQLRFMEDELGQQG 162 Query: 152 YLAGDEYTIADI-ASFPWTRSWKNQGIELSEYPNVQRWFDAINERPAVQRGV 202 + AG +++ ADI SFP + + +G YPN+ ++ +AI+ RPA QR + Sbjct: 163 WFAGAQFSGADIQMSFP-VEALRARGGLDGRYPNLLKYLEAIHARPAYQRAL 213 >gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 56.2 bits (134), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 55/195 (28%), Positives = 84/195 (43%), Gaps = 11/195 (5%) Query: 11 NGHKVHIMLEELGLDYRAHAIDIGAGDQFADAFLKISPNNKIPAIVDSDGPDGEPISLFE 70 N KV ELG+ + G AFL ++PN +P +++ DG L+E Sbjct: 12 NVRKVLWACAELGIAFEREDWGAGFRPTSEPAFLAMNPNAMVP-VIEEDG-----FVLWE 65 Query: 71 SGAILIYLAGKTG--KLLGNTDRERFNVLQWVMFQMGGLGPMLGQAHHFRIYAPEKIEYA 128 S AI+ YLA + G L + R V QW+ +Q L A+ + A Sbjct: 66 SNAIIRYLANRHGGAALYPLQAQARARVDQWIDWQASDLNRAWSYAYLTLVRKSPAHGNA 125 Query: 129 VNRYSNEA--KRLYGVLDKQLSKTA-YLAGDEYTIADIASFPWTRSWKNQGIELSEYPNV 185 ++ A R VL++QL + Y+AG E+T+ADI+ W E P V Sbjct: 126 QQVAASIADWTRFMEVLEQQLQHSGGYVAGAEFTLADISVGLSVNRWFGTPFEHPPLPAV 185 Query: 186 QRWFDAINERPAVQR 200 ++D + ER +R Sbjct: 186 AAYYDRLTERDGFRR 200 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 793,902 Number of extensions: 34503 Number of successful extensions: 112 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 6 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)