BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0062.p (306 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313151|ref|YP_003777243.1| glutamate/aspartate ABC transpo... 192 3e-50 gi|300312815|ref|YP_003776907.1| amino acid ABC transporter/sign... 170 2e-43 gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/sign... 77 1e-15 gi|300311002|ref|YP_003775094.1| amino acid ABC transporter/sign... 71 1e-13 gi|300313169|ref|YP_003777261.1| amino acid transport periplasmi... 71 1e-13 >gi|300313151|ref|YP_003777243.1| glutamate/aspartate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 315 Score = 192 bits (488), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 97/268 (36%), Positives = 155/268 (57%) Query: 34 GTLEKIRRTNAVVIAYLNGTTPFAFLGDDKLVTGYTIELCRNIAEGLRKELKLPHMQVQF 93 G L+KI+ + + + + + PF++L D + GY+I+LC A ++K+L L + ++ Sbjct: 27 GRLKKIKDSGTLTLGVRDSSIPFSYLDDKQSYQGYSIDLCLKAATAIQKKLGLTSLNIKM 86 Query: 94 MEVDTVTRFIALDQGKADLECSTSTNNAERRKIAAFTVPHFFAAVRMLVKKDSGIRNWED 153 + V + TR + G D+ C ++TNN ER AF+V F A + L KK + ++ ED Sbjct: 87 VPVTSATRIPLIANGTTDISCDSATNNQERWNQVAFSVTEFVTANKFLSKKAANLKTLED 146 Query: 154 LRGKTVVVPRNTPMVKLLEQRSKIRSLNVRIVEVAYNEDAFDSVEKGKADAFVMEDVLLR 213 L+GKTVV T +K + + R+L + I+ + +AF VE G+A AFVM+D+LL Sbjct: 147 LKGKTVVSTSGTSNLKQITALNAERNLGMNILAAKDHAEAFLMVETGRAAAFVMDDILLS 206 Query: 214 RYLLSVPQPKNYQVVGEALSLEPYGIMMRKGDPEFKKFVDLQMVRIVRSGEIYRIYDRWF 273 + P +YQV EALS+EPYG+++ KGD EFK VD + + + +I +IY +WF Sbjct: 207 SLAANSKAPGDYQVSSEALSVEPYGLILPKGDKEFKTVVDDALTVVYKGDDIKKIYAKWF 266 Query: 274 VQPISAKVPSMDMPMGFILRDTLRYPTD 301 PI K ++++PM L+ L PTD Sbjct: 267 QSPIPPKGVNLNVPMSPQLQAVLAKPTD 294 >gi|300312815|ref|YP_003776907.1| amino acid ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 305 Score = 170 bits (430), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 86/271 (31%), Positives = 149/271 (54%) Query: 35 TLEKIRRTNAVVIAYLNGTTPFAFLGDDKLVTGYTIELCRNIAEGLRKELKLPHMQVQFM 94 TL+KI+ N + I + + PF++ +++ V GY+ +LC + + ++ ++ ++V+ + Sbjct: 35 TLQKIKDANLIAIGHRTSSIPFSYYDENQQVIGYSQDLCNKVIDAVKTKIGAAKLEVRMV 94 Query: 95 EVDTVTRFIALDQGKADLECSTSTNNAERRKIAAFTVPHFFAAVRMLVKKDSGIRNWEDL 154 V + R L G DLEC ++N R + +F F A+ R+L K+DSGI+++ DL Sbjct: 95 PVTSQNRTPLLQNGTIDLECGVTSNLKSRWQQVSFATNFFVASSRILTKRDSGIKDFPDL 154 Query: 155 RGKTVVVPRNTPMVKLLEQRSKIRSLNVRIVEVAYNEDAFDSVEKGKADAFVMEDVLLRR 214 GK VV T +LL ++ R + ++I +AF ++ G+ A+VM+DVLL Sbjct: 155 AGKAVVTNAGTTSEQLLRNLNEHRKMGMQIQSAKDYGEAFLILQSGRVAAYVMDDVLLAG 214 Query: 215 YLLSVPQPKNYQVVGEALSLEPYGIMMRKGDPEFKKFVDLQMVRIVRSGEIYRIYDRWFV 274 + +P ++ + G EPY M+R+ DP+FK+ VD + +++SGEI +IY +WF Sbjct: 215 ARTTAQKPNDWVLTGNPFGAEPYAFMVRRDDPQFKQLVDDTLSGLMKSGEIKKIYAKWFT 274 Query: 275 QPISAKVPSMDMPMGFILRDTLRYPTDKVGD 305 PI K S + PM + P+DK D Sbjct: 275 FPIPPKNVSFNFPMSDTVMKLYATPSDKPMD 305 >gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 271 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 68/275 (24%), Positives = 120/275 (43%), Gaps = 11/275 (4%) Query: 1 MMTRYA-SLATTRWIIKTVVLGLCMALQMQARAEGTLEKIRRTNAVVIAYLNGTTPFAFL 59 M TR+A +LA T + + L C + ++ + V+ + PF+ Sbjct: 4 MFTRHARALALTAGFVGMLGLAACGKQEDKSATAAAPQAAAPARVYVVGVESAYAPFSSE 63 Query: 60 GDDKLVTGYTIELCRNIAEGLRKELKLPHMQVQFMEVDTVTRFIALDQGKADLECSTSTN 119 + K V G+ I++ + +A+ + ++V+F+ F L QG DL S T Sbjct: 64 NEQKDVVGFDIDIMKALAKKI-------GIEVKFVPTPFEGFFNFLAQGDRDLLISAITI 116 Query: 120 NAERRKIAAFTVPHFFAAVRM-LVKKDSGIRNWEDLRGKTVVVPRNTPMVKLLEQRSKIR 178 ER+K AF+ P+F A + L D+ + EDL+ TV T +L++Q Sbjct: 117 TDERKKSVAFSEPYFVATQTIALPAADTKVSKMEDLKPLTVGTQSATSGDELVQQVLGKN 176 Query: 179 SLNVRIVEVAYNEDAFDSVEKGKADAFVMEDVLLRRYLLSVPQPKNYQVVGEALSLEPYG 238 S ++ + A +E G DA V ++ +++ Y+ + P K V + E YG Sbjct: 177 SAKIKRFD--STPLALKELESGGVDAVVADEPVVKNYIANNPNSKLRTVTDPSFPKEDYG 234 Query: 239 IMMRKGDPEFKKFVDLQMVRIVRSGEIYRIYDRWF 273 I +RK DPE ++ + + G I ++F Sbjct: 235 IAVRKDDPELLAKINKGLAEMKADGSFAAISAQYF 269 >gi|300311002|ref|YP_003775094.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 279 Score = 70.9 bits (172), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 65/269 (24%), Positives = 115/269 (42%), Gaps = 16/269 (5%) Query: 16 KTVVLGLCMALQMQARAEGTLEKIRRTNAVVIAYLNGTTPFAFLG-DDKLVTGYTIELCR 74 K +L C AL L +I+ +V A L P F + V G+ +++C Sbjct: 13 KGSLLVACAALFPGLAQADRLAEIQARGTLVCATLANNEPLGFPDPQSRQVVGFDVDMCA 72 Query: 75 NIAEGLRKELKLPHMQVQFMEVDTVTRFIALDQGKADLECSTSTNNAERRKIAAFTVPHF 134 +A L +Q++ V R AL +G+ADL + ER + FT H+ Sbjct: 73 AVARRL-------GVQMEQKSVTVEGRLPALIEGQADLVSAALGYTRERARQIDFTASHY 125 Query: 135 FAAVRMLVKKDSGIRNWEDLRGKTVVVPR-NTPMVKLLEQRSKIRSLNVRIVEVAYNEDA 193 ++++V+ DSG+ DL K + R +TP EQ + + A A Sbjct: 126 QNPIKLIVQSDSGLNLISDLADKRISANRSSTP-----EQAVRRVLPKAMLQTFADTPQA 180 Query: 194 FDSVEKGKADAFVMEDVLLRRYLLSVPQPKNYQVVGEALSLEPYGIMMRKGDPEFKKFVD 253 F ++ +G+AD + R++ ++ V ALS EP + ++KG+ + V+ Sbjct: 181 FLALAQGRADGLAISMPSGIRFVNE--SAGRFKFVEGALSWEPTALGVKKGERRLRDAVN 238 Query: 254 LQMVRIVRSGEIYRIYDRWFVQPISAKVP 282 + + + GEI ++ +WF +P Sbjct: 239 AALAALEKDGEIDALWTKWFGPKTKFNIP 267 >gi|300313169|ref|YP_003777261.1| amino acid transport periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 70.9 bits (172), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 63/239 (26%), Positives = 105/239 (43%), Gaps = 12/239 (5%) Query: 39 IRRTNAVVIAYLNGTTPFAFLGDDKLVTGYTIELCRNIAEGLRKELKLPHMQVQFMEVDT 98 I+ V++A G PF G +E R +A+ L EL++ ++V Sbjct: 33 IKARKKVLVAIDLGAPPFGMTNAKLEPQGSDVETARLLAKDLGVELEI-------VQVTG 85 Query: 99 VTRFIALDQGKADLECSTSTNNAERRKIAAFTVPHFFAAVRMLVKKDSGIRNWEDLRGKT 158 R L GKAD+ S+ + ER+K+ AFT P+ + + K I++ DL GK Sbjct: 86 PNRIPFLMTGKADMVISSFSITPERQKVVAFTQPYGASQFVVAAPKGENIKSLNDLVGKR 145 Query: 159 VVVPRNTPMVKLLEQRSKIRSLNVRIVEVAYNEDAFDSVEKGKADAFVMEDVLLRRYLLS 218 V V R LL + I IV + ++ G+ DA + L Sbjct: 146 VAVVRGNMQDSLL---TPIAPKGTNIVRFDDDATVTAAIISGQVDALCTPNSLASAIAKQ 202 Query: 219 VPQPKNYQVVGEALSLEPYGIMMRKGDPEFKKFVDLQMVRIVRSGEIYRIYDRWFVQPI 277 P KN + A+ PY I +RKG+P +K+++ + +++G++ IY +W P+ Sbjct: 203 NPS-KNLE-TKFAIKDIPYAIGLRKGEPALEKWLNEWISANLKNGKLPGIYQQWVGSPM 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 863,884 Number of extensions: 32826 Number of successful extensions: 118 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 13 Length of query: 306 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 221 Effective length of database: 1,217,395 Effective search space: 269044295 Effective search space used: 269044295 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)