BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0061.p (341 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313151|ref|YP_003777243.1| glutamate/aspartate ABC transpo... 575 e-165 gi|300312815|ref|YP_003776907.1| amino acid ABC transporter/sign... 253 2e-68 gi|300313169|ref|YP_003777261.1| amino acid transport periplasmi... 74 2e-14 gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/sign... 69 7e-13 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 64 1e-11 >gi|300313151|ref|YP_003777243.1| glutamate/aspartate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 315 Score = 575 bits (1483), Expect = e-165, Method: Compositional matrix adjust. Identities = 283/315 (89%), Positives = 294/315 (93%) Query: 27 MKLLKFAGVLVGASVLMGSVHAEEFTGRLKKIKETGTLTLGVRDASIPFSYLDDKQSYQG 86 MKLLK AGVLVGASVLM SVHAEEFTGRLKKIK++GTLTLGVRD+SIPFSYLDDKQSYQG Sbjct: 1 MKLLKLAGVLVGASVLMSSVHAEEFTGRLKKIKDSGTLTLGVRDSSIPFSYLDDKQSYQG 60 Query: 87 YSIDLCLKAATAIQKKLGLTSLNVKMVPVTSATRIPLIANGTTDISCDSATNNMERQKQV 146 YSIDLCLKAATAIQKKLGLTSLN+KMVPVTSATRIPLIANGTTDISCDSATNN ER QV Sbjct: 61 YSIDLCLKAATAIQKKLGLTSLNIKMVPVTSATRIPLIANGTTDISCDSATNNQERWNQV 120 Query: 147 AFAVTEFVTANRFLTKKAANLKTLDDLKGKTIVSTSGTSNLKQITTLNTERNLGMNILAA 206 AF+VTEFVTAN+FL+KKAANLKTL+DLKGKT+VSTSGTSNLKQIT LN ERNLGMNILAA Sbjct: 121 AFSVTEFVTANKFLSKKAANLKTLEDLKGKTVVSTSGTSNLKQITALNAERNLGMNILAA 180 Query: 207 KDHAEAFLMVETGRAVAFVMDDILLSSLAANSKAPGDYVVSSEALSVEPYGLILPLGDAA 266 KDHAEAFLMVETGRA AFVMDDILLSSLAANSKAPGDY VSSEALSVEPYGLILP GD Sbjct: 181 KDHAEAFLMVETGRAAAFVMDDILLSSLAANSKAPGDYQVSSEALSVEPYGLILPKGDKE 240 Query: 267 FKKVVDDALVNVYKGDDIKKIYAKWFQSPIPPKGVNLNVPMSPQLQAVFAKPTDSGEPSA 326 FK VVDDAL VYKGDDIKKIYAKWFQSPIPPKGVNLNVPMSPQLQAV AKPTDS +P+A Sbjct: 241 FKTVVDDALTVVYKGDDIKKIYAKWFQSPIPPKGVNLNVPMSPQLQAVLAKPTDSYDPAA 300 Query: 327 YAAVPEAQKQSSKKK 341 YA VPEAQK KKK Sbjct: 301 YAVVPEAQKNMGKKK 315 >gi|300312815|ref|YP_003776907.1| amino acid ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 305 Score = 253 bits (645), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 118/266 (44%), Positives = 171/266 (64%) Query: 55 LKKIKETGTLTLGVRDASIPFSYLDDKQSYQGYSIDLCLKAATAIQKKLGLTSLNVKMVP 114 L+KIK+ + +G R +SIPFSY D+ Q GYS DLC K A++ K+G L V+MVP Sbjct: 36 LQKIKDANLIAIGHRTSSIPFSYYDENQQVIGYSQDLCNKVIDAVKTKIGAAKLEVRMVP 95 Query: 115 VTSATRIPLIANGTTDISCDSATNNMERQKQVAFAVTEFVTANRFLTKKAANLKTLDDLK 174 VTS R PL+ NGT D+ C +N R +QV+FA FV ++R LTK+ + +K DL Sbjct: 96 VTSQNRTPLLQNGTIDLECGVTSNLKSRWQQVSFATNFFVASSRILTKRDSGIKDFPDLA 155 Query: 175 GKTIVSTSGTSNLKQITTLNTERNLGMNILAAKDHAEAFLMVETGRAVAFVMDDILLSSL 234 GK +V+ +GT++ + + LN R +GM I +AKD+ EAFL++++GR A+VMDD+LL+ Sbjct: 156 GKAVVTNAGTTSEQLLRNLNEHRKMGMQIQSAKDYGEAFLILQSGRVAAYVMDDVLLAGA 215 Query: 235 AANSKAPGDYVVSSEALSVEPYGLILPLGDAAFKKVVDDALVNVYKGDDIKKIYAKWFQS 294 ++ P D+V++ EPY ++ D FK++VDD L + K +IKKIYAKWF Sbjct: 216 RTTAQKPNDWVLTGNPFGAEPYAFMVRRDDPQFKQLVDDTLSGLMKSGEIKKIYAKWFTF 275 Query: 295 PIPPKGVNLNVPMSPQLQAVFAKPTD 320 PIPPK V+ N PMS + ++A P+D Sbjct: 276 PIPPKNVSFNFPMSDTVMKLYATPSD 301 >gi|300313169|ref|YP_003777261.1| amino acid transport periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 63/226 (27%), Positives = 106/226 (46%), Gaps = 16/226 (7%) Query: 74 PFSYLDDKQSYQGYSIDLCLKAATAIQKKLGLTSLNVKMVPVTSATRIPLIANGTTDISC 133 PF + K QG ++ A + K LG+ +++V VT RIP + G D+ Sbjct: 49 PFGMTNAKLEPQGSDVE----TARLLAKDLGV---ELEIVQVTGPNRIPFLMTGKADMVI 101 Query: 134 DSATNNMERQKQVAFAVTEFVTANRFLT--KKAANLKTLDDLKGKTIVSTSGTSNLKQIT 191 S + ERQK VAF T+ A++F+ K N+K+L+DL GK + G +T Sbjct: 102 SSFSITPERQKVVAF--TQPYGASQFVVAAPKGENIKSLNDLVGKRVAVVRGNMQDSLLT 159 Query: 192 TLNTERNLGMNILAAKDHAEAFLMVETGRAVAFVMDDILLSSLAANSKAPGDYVVSSEAL 251 + + G NI+ D A + +G+ A + L S++A + P + + A+ Sbjct: 160 PIAPK---GTNIVRFDDDATVTAAIISGQVDALCTPNSLASAIAKQN--PSKNLETKFAI 214 Query: 252 SVEPYGLILPLGDAAFKKVVDDALVNVYKGDDIKKIYAKWFQSPIP 297 PY + L G+ A +K +++ + K + IY +W SP+P Sbjct: 215 KDIPYAIGLRKGEPALEKWLNEWISANLKNGKLPGIYQQWVGSPMP 260 >gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 260 Score = 68.6 bits (166), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 78/278 (28%), Positives = 118/278 (42%), Gaps = 28/278 (10%) Query: 27 MKLLKFA-GVLVGASVLMGSVHAEEFTGRLKKIKETGTLTLGVRDASIPFSYLDDKQSYQ 85 MKL K G++ GA + SV + L I++ GTL + V PF +D Q Sbjct: 1 MKLTKLLLGLMAGAMMFSASVARAD---ALDDIQKHGTLRVAVPADFPPFGSVDADLKPQ 57 Query: 86 GYSIDLCLKAATAIQKKLGLTSLNVKMVPVTSATRIPLIANGTTDISCDSATNNMERQKQ 145 G ID+ AT I KKLG+ V+++PV+SA RIP + D+ S N ER+K Sbjct: 58 GLDIDV----ATLIAKKLGV---KVELIPVSSANRIPYLTTKKVDLVISSMGKNAEREKV 110 Query: 146 VAFAVTEFVTANRFLTKKAANLKTLDDLKGKTIVSTSGTSNLKQITTL-----NTERNLG 200 + F+ N ++K+ DL GKTI T G+ ++T + +R Sbjct: 111 IDFSAAYAPFFNGVFGPADLSVKSAADLAGKTIAVTRGSVEDLELTKIVPASATVKRYED 170 Query: 201 MNILAAKDHAEAFLMVETGRAVAFVMDDILLSSLAAN-SKAPGDYVVSSEALSVEPYGLI 259 N + + +V TG VA + +A N K P + ++ P + Sbjct: 171 NNSTISSFLSGQVQLVATGNVVAAAI-------IARNPPKKPETKFLIKDS----PCFIG 219 Query: 260 LPLGDAAFKKVVDDALVNVYKGDDIKKIYAKWFQSPIP 297 L G+ + VD L K + I KW P+P Sbjct: 220 LNKGEKKLQDKVDAILAESKKDGSLSAISQKWLGMPLP 257 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 73/266 (27%), Positives = 116/266 (43%), Gaps = 32/266 (12%) Query: 36 LVGASVLMGSVHAEEFTGRLKKIKETGTLTLGVRDASIPFSYLDDKQSYQGYSIDLCLKA 95 LV SV+ S AE TL + A +PF + D Q Y G+ IDL Sbjct: 13 LVALSVVGASARAE-------------TLVVACDTAFVPFEFKDRNQ-YVGFDIDLW--- 55 Query: 96 ATAIQKKLGLTSLNVKMVPVTSATRIPLIANGTTDISCDSATNNMERQKQVAFAVTEFVT 155 AI K +G+T K+ P+ IP + D++ T ER+K + F+ + + Sbjct: 56 -DAIAKDIGVT---YKLQPMDFNGIIPALQTKNVDVALAGITIKDERKKVIDFSDGYYDS 111 Query: 156 ANRFLTKKAANLKTLDDLKGKTIVSTSGTS--NLKQITTLNTERNLGMNILAAKDHAEAF 213 + +++K++ DL GK + SGTS + + TE + NI A+ Sbjct: 112 GFSLMVPVGSSIKSVADLAGKALAVKSGTSAFDYAKANFKATELHQFPNI------DNAY 165 Query: 214 LMVETGRAVAFVMDDILLSSLAANSKAPGDYVVSSEALSVEPYGLILPLGDAAFKKVVDD 273 L ++TGR A + D + A + VV ++ ++ + YG+ P G K V+ Sbjct: 166 LELQTGRVDAAMHDTPNVLYYIATAGKGRAKVVGTQMMAHQ-YGIGFPKGSPLVPK-VNA 223 Query: 274 ALVNVYKGDDIKKIYAKWFQSPIPPK 299 AL + +IY KWF + PPK Sbjct: 224 ALAKIKADGRYAEIYRKWFGAE-PPK 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 942,867 Number of extensions: 36032 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 12 Length of query: 341 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 255 Effective length of database: 1,212,660 Effective search space: 309228300 Effective search space used: 309228300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)