BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0060.p (249 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313150|ref|YP_003777242.1| glutamate/aspartate ABC transpo... 452 e-128 gi|300312813|ref|YP_003776905.1| glutamate/aspartate ABC transpo... 229 2e-61 gi|300312812|ref|YP_003776904.1| glutamate/aspartate ABC transpo... 94 1e-20 gi|300313536|ref|YP_003777628.1| amino acid ABC transporter perm... 92 3e-20 gi|300313611|ref|YP_003777703.1| amino acid ABC transporter perm... 88 7e-19 >gi|300313150|ref|YP_003777242.1| glutamate/aspartate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 249 Score = 452 bits (1163), Expect = e-128, Method: Compositional matrix adjust. Identities = 220/249 (88%), Positives = 236/249 (94%) Query: 1 MHYNWNWGIFWEQSPDGIPYIDTLLIGLKWTIATAVLAWIMALILGTVIGTIRTTQKPWA 60 MHYNWNWGIFWE SPDGIPYIDTLL GLKWT+ATA AWIMALILGT+ GT+RTT KPW Sbjct: 1 MHYNWNWGIFWEMSPDGIPYIDTLLAGLKWTLATAACAWIMALILGTIFGTLRTTTKPWV 60 Query: 61 VKIANGYVELFRNIPLLVQMFLWYFVMPELVPAAMGDWLKSLPEASFVTAFLALGFFTSS 120 V+IANGYVELFRNIPLLVQMFLWYFVMPEL+PA +GDW+KSLP+ASFVTA LALGFFTSS Sbjct: 61 VRIANGYVELFRNIPLLVQMFLWYFVMPELLPAFIGDWIKSLPDASFVTATLALGFFTSS 120 Query: 121 RVAVQVSTGINALPRGQRMAGAALGLTPVQTYRYVLLPMAFRIIIPALTNEFAAIIKNSS 180 RVAVQV+TGI ALPRGQRMAGAALGLTPVQTYRYVLLPMAFRIIIPALTNEFAAIIKNSS Sbjct: 121 RVAVQVTTGIQALPRGQRMAGAALGLTPVQTYRYVLLPMAFRIIIPALTNEFAAIIKNSS 180 Query: 181 VALTIGLVELTAATYSMREFTFQTFESLTGATVIYIIISVIALMLARFLERVTAVPGYIT 240 VALTIGLVELTAATYSMREFTFQTFE+LTGAT+IY+IISVIAL +AR LE+VTAVPGYIT Sbjct: 181 VALTIGLVELTAATYSMREFTFQTFEALTGATIIYVIISVIALFMARLLEKVTAVPGYIT 240 Query: 241 TGSSSSGGH 249 GS+S+GGH Sbjct: 241 GGSTSAGGH 249 >gi|300312813|ref|YP_003776905.1| glutamate/aspartate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 241 Score = 229 bits (583), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 121/239 (50%), Positives = 161/239 (67%), Gaps = 4/239 (1%) Query: 1 MHYNWNWGIFWEQSPDGIPYIDTLLIGLKWTIATAVLAWIMALILGTVIGTIRTTQKPWA 60 M Y W W +F + G Y+D LL GL T+A + AWI+AL+LGT +G +RT Sbjct: 1 MAYQWQWSVFLQAPSTGQTYLDWLLSGLGVTVALGLSAWILALVLGTFLGILRTLPNRAL 60 Query: 61 VKIANGYVELFRNIPLLVQMFLWYFVMPELVPAAMGDWLKSLPEAS--FVTAFLALGFFT 118 A YVELFRN+PLLVQ+F+WYF MPEL+P GD +K+LP + FV A L LG FT Sbjct: 61 SGFAACYVELFRNVPLLVQIFIWYFAMPELLP--WGDAVKALPPMTQQFVMAMLCLGMFT 118 Query: 119 SSRVAVQVSTGINALPRGQRMAGAALGLTPVQTYRYVLLPMAFRIIIPALTNEFAAIIKN 178 ++RV QV +GI +LPRGQ+ AG ALGLT QTY +VLLPMA RI++P LT+EF I KN Sbjct: 119 AARVCEQVRSGIESLPRGQKGAGLALGLTLGQTYLFVLLPMAMRIVLPPLTSEFLNIFKN 178 Query: 179 SSVALTIGLVELTAATYSMREFTFQTFESLTGATVIYIIISVIALMLARFLERVTAVPG 237 S+VA TIGL+EL A + ++T Q +E+ T+ Y++I++ + L R LER T++PG Sbjct: 179 SAVASTIGLLELAAQGRQLVDYTAQPYEAFIAVTLAYMLINICVMSLMRILERKTSLPG 237 >gi|300312812|ref|YP_003776904.1| glutamate/aspartate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 227 Score = 93.6 bits (231), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 68/213 (31%), Positives = 113/213 (53%), Gaps = 9/213 (4%) Query: 22 DTLLIGLKWTIATAVLAWIMALILGTVIGTIRTTQKPWAVKIANGYVELFRNIPLLVQMF 81 D LL+ LK T+ +A + ++ GT++ +R ++ A GYV LFR+IPL V + Sbjct: 20 DGLLVSLKITL----VAIVFGMVWGTLLAMMRLSRHSPLQWFAAGYVNLFRSIPL-VMVL 74 Query: 82 LWYF-VMPELVPAAMGDWLKSLPEASFVTAFLALGFFTSSRVAVQVSTGINALPRGQRMA 140 LW+F ++P+L+ G L + +A +A F ++ A + GIN++ RGQ A Sbjct: 75 LWFFLIVPQLLQKLFG--LSPANDLRMTSALVAFALFEAAYYAEIIRAGINSVSRGQMAA 132 Query: 141 GAALGLTPVQTYRYVLLPMAFRIIIPALTNEFAAIIKNSSVALTIGLVELTAATYSMREF 200 ALGLT Q V+LP AFR ++P L + + +++S+ L + YS+ E Sbjct: 133 AYALGLTYPQAMGMVILPQAFRNMVPLLLTQGIVLFQDTSLVYVSALADFFGRAYSIGER 192 Query: 201 TFQTFESLTGATVIYIIISV-IALMLARFLERV 232 + E L A +Y II ++LM+ R+ ++V Sbjct: 193 NGRIIEMLFVAGAVYFIICFSMSLMVKRYRKKV 225 >gi|300313536|ref|YP_003777628.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 223 Score = 92.4 bits (228), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 61/211 (28%), Positives = 113/211 (53%), Gaps = 12/211 (5%) Query: 20 YIDTLLIGLKWTIATAVLAWIMALILGTVIGTIRTTQKP-WAVKIANGYVELFRNIPLLV 78 Y D ++ G+ TI + ++ + +G + G TQ P W + +GYVE+ RN P LV Sbjct: 13 YTDVIVKGVATTIELIAIGGVLGVAVG-IFGAWARTQGPGWLKPVVSGYVEIIRNTPFLV 71 Query: 79 QMFLWYFVMPELVPAAMGDWLKSLPEASFVTAFLALGFFTSSRVAVQVSTGINALPRGQR 138 Q+F +F +P L D S A+ V + LG +++ + G+ A+PRGQ Sbjct: 72 QLFFIFFGLPSL------DIHISEITAAIVAMVINLGAYSTE----IIRAGVQAIPRGQI 121 Query: 139 MAGAALGLTPVQTYRYVLLPMAFRIIIPALTNEFAAIIKNSSVALTIGLVELTAATYSMR 198 A +L ++ +Q +R+++L A + I PAL+++ ++ S+V I + EL+ A ++ Sbjct: 122 EAAQSLSMSRMQIFRHIILRPALQKIWPALSSQVVIVMLGSAVCSQIAVEELSFAANFIQ 181 Query: 199 EFTFQTFESLTGATVIYIIISVIALMLARFL 229 F+ FE+ AT IY++++++ + R + Sbjct: 182 GRNFRAFEAYIVATAIYLVLAILLRQVLRLI 212 >gi|300313611|ref|YP_003777703.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 247 Score = 87.8 bits (216), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 66/226 (29%), Positives = 121/226 (53%), Gaps = 18/226 (7%) Query: 27 GLKWTIATAVLAWIMALILGTVIGTIRTTQK-----PWAVK----IANGYVELFRNIPLL 77 G+ T+ ++++ ++ ++G ++G +R + W +K +A YV FR PL Sbjct: 18 GVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLKSMRWLAGFYVAFFRGTPLF 77 Query: 78 VQMFLWYF-VMPELVPAAMG-----DWLKSLPE--ASFVTAFLALGFFTSSRVAVQVSTG 129 VQ+ L +F VMP LV G + +++ + +F++ +AL + ++ G Sbjct: 78 VQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTVALSLNAGAYISEIFRAG 137 Query: 130 INALPRGQRMAGAALGLTPVQTYRYVLLPMAFRIIIPALTNEFAAIIKNSSVALTIGLVE 189 I ++ RGQ A A+LG+ T RYV+LP AFR ++P L NE ++K+SS+ IGL E Sbjct: 138 IQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEAITLLKDSSLVSAIGLAE 197 Query: 190 LTAATYSMREFTFQTFESLTGATVIYIIISV-IALMLARFLERVTA 234 L A ++ + +E +V+Y +++ +AL++AR + +A Sbjct: 198 LAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESKYSA 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 724,047 Number of extensions: 28120 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 19 Length of query: 249 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 166 Effective length of database: 1,226,865 Effective search space: 203659590 Effective search space used: 203659590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)