BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0044.p (433 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313138|ref|YP_003777230.1| glutamate-1-semialdhyde 2,1-ami... 696 0.0 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 147 2e-36 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 119 6e-28 gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 116 3e-27 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 112 8e-26 >gi|300313138|ref|YP_003777230.1| glutamate-1-semialdhyde 2,1-aminomutase [Herbaspirillum seropedicae SmR1] Length = 427 Score = 696 bits (1797), Expect = 0.0, Method: Compositional matrix adjust. Identities = 355/424 (83%), Positives = 385/424 (90%) Query: 8 NTNESLFARAQKTTPGGVNSPVRAFRSVGGTPRFITKAQGPYFWDAEERRYIDYIGSWGP 67 TN SLFARAQ++TPGGVNSPVRAFRSVGGTPRFI +A+GP+FWDAE +RYIDYIGSWGP Sbjct: 3 KTNASLFARAQQSTPGGVNSPVRAFRSVGGTPRFIERAEGPWFWDAEGKRYIDYIGSWGP 62 Query: 68 AIVGHAHPKVVQAVQEAAARGLSFGAPTEGEIDMAEEICKLVPSIEQVRLVSSGTEATMS 127 AIVGHAHP+V+QAVQ+AAARGLSFGAPTE EI+MAEEI KLVPSIEQ+RLVSSGTEATMS Sbjct: 63 AIVGHAHPEVIQAVQQAAARGLSFGAPTEAEIEMAEEIIKLVPSIEQIRLVSSGTEATMS 122 Query: 128 ALRLARGATKRDKIVKFEGCYHGHADSLLVKAGSGLLTFGNPTSSGVPEDFVKHTLVLDY 187 ALRLARGAT RDKI+KFEGCYHGHADSLLVKAGSGLLTFGNPTS+GVPEDF KHTLVLDY Sbjct: 123 ALRLARGATGRDKIIKFEGCYHGHADSLLVKAGSGLLTFGNPTSAGVPEDFAKHTLVLDY 182 Query: 188 NNTQQLEEAFKAAGNDIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYGAILIFDEVMS 247 N+ QLEEAFK AGN+IACVIVEPVAGNMNLV A+ EFL+TMRRLCTEYGAILIFDEVMS Sbjct: 183 NDAAQLEEAFKTAGNEIACVIVEPVAGNMNLVRASDEFLRTMRRLCTEYGAILIFDEVMS 242 Query: 248 GFRVALGGAQSLYDITPDLTALGKVIGGGLPVAAFGGRADIMRHLAPLGGVYQAGTLSGN 307 GFRVA GGAQ L I PDLTALGKVIGGGLPVAAFGGRA++M+HLAPLGGVYQAGTLSGN Sbjct: 243 GFRVARGGAQELNGIVPDLTALGKVIGGGLPVAAFGGRAEVMKHLAPLGGVYQAGTLSGN 302 Query: 308 PVAVAAGLTTLKIIQEAGFYEQLSAQTRKLADGLSEAARDAGVTFSADTMGGMFGLYFSA 367 PV VAAG+ TLKIIQ+ FY LS QTRKLADGL+ AA+ AGVTF+AD +GGMFGLYF A Sbjct: 303 PVTVAAGMATLKIIQQPDFYTNLSTQTRKLADGLAAAAQAAGVTFAADAIGGMFGLYFDA 362 Query: 368 AVPTSYAEVMQGDKERFNMFFHAMLDQGVYLAPSAFEAGFVSAQHDDAVIAETLVAARTA 427 VPTSYAEVMQGDKERFN FFH MLD GVY APSAFEAGFVSAQH DA+I ET+ AA A Sbjct: 363 KVPTSYAEVMQGDKERFNRFFHKMLDAGVYFAPSAFEAGFVSAQHSDAIIEETVAAAARA 422 Query: 428 FAEI 431 F+E+ Sbjct: 423 FSEL 426 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 147 bits (371), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 110/355 (30%), Positives = 173/355 (48%), Gaps = 32/355 (9%) Query: 7 RNTNESLFARAQKTTPGGVNSPVRAFRSVGGTPRFITKAQGPYFWDAEERRYIDYIGSWG 66 + N+ L R TP GV + +A WD E RR+ID+ Sbjct: 4 KANNQELQQRKNAATPRGVGVMCDFYAE---------RAANAELWDVEGRRFIDFAAGIA 54 Query: 67 PAIVGHAHPKVVQAVQEAAARGLSFG---APTEGEIDMAEEICKLVPS--IEQVRLVSSG 121 GH HPK++ A++ + P +++AE I +L P ++ S+G Sbjct: 55 VLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVELAERINRLTPGNYPKKTAFFSTG 114 Query: 122 TEATMSALRLARGATKRDKIVKFEGCYHGHAD---SLLVKAGSGLLTFGNPTSSGV---P 175 EA +A+++AR T R ++ F G +HG +L K L FG P V P Sbjct: 115 AEAVENAIKIARAHTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLGFG-PFPGDVFHAP 173 Query: 176 EDFVKHTLVLDYNNTQQLEEAFKA--AGNDIACVIVEPVAGNMNLVPATAEFLQTMRRLC 233 H + + + + ++ FK+ +A +I+EPV G A A+F++ +R LC Sbjct: 174 YPSALHGITSE-DALEAVKGLFKSDIEAKRVAAIILEPVQGEGGFYAAPADFMRGLRALC 232 Query: 234 TEYGAILIFDEVMSGF-RVALGGAQSLYDITPDLTALGKVIGGGLPVAAFGGRADIMRHL 292 E+G +LI DEV SG+ R A YD+ PDL + K + GG+P++A GRA+IM Sbjct: 233 DEHGILLIADEVQSGYGRTGKLFAMEHYDVLPDLMTMAKSLAGGMPLSAVNGRAEIMDAP 292 Query: 293 APLGGVYQAGTLSGNPVAVAAGLTTLKIIQEAGFYEQLSAQTRKLADGLSEAARD 347 AP GG+ GT +GNP+A+A+ L L +++E EQL + ++L D L E ++ Sbjct: 293 AP-GGL--GGTYAGNPLAIASALAVLDVMEE----EQLVTRGQRLGDKLQEHLKE 340 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 119 bits (297), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 106/353 (30%), Positives = 165/353 (46%), Gaps = 44/353 (12%) Query: 28 PVRAFRSVGGTPRFITKAQGPYFWDAEERRYIDYIGSWGPAIVGHAHPKVVQAVQEAAAR 87 P A R+ PR + A+G ++ D +D +GHAHPK+V AVQ++ A Sbjct: 18 PFTANRNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSAVQKSVAT 77 Query: 88 -----GLSFGAPTEGEIDMAEEICKLVPS-IEQVRLVSSGTEATMSALRLA------RGA 135 G P+ E +AE + + V SG+EA +AL++A +G Sbjct: 78 LDYAPSFQLGHPSAFE--LAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGN 135 Query: 136 TKRDKIVKFEGCYHG--HADSLLVKAGSGLLTFGNPTSSGVPEDFVKHTLVLDYNNTQQL 193 R +++ E YHG + G TF GV D + HT L+ N + Sbjct: 136 ATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGV--DHLPHTHNLEKNAFTKG 193 Query: 194 EEAFKAA-------------GNDIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYGAIL 240 E + A ++IA VIVEPVAG+ ++ +L+ +R +CT++G +L Sbjct: 194 EPEYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHGILL 253 Query: 241 IFDEVMSGF-RVALGGAQSLYDITPDLTALGK-VIGGGLPVAAFGGRADIMRHLAPLGG- 297 IFDEV++GF R+ A +D+ PD+ K + G +P+ A + +I H A + G Sbjct: 254 IFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEI--HDAFMNGP 311 Query: 298 ----VYQAGTLSGNPVAVAAGLTTLKIIQEAGFYEQLSAQTRKLADGLSEAAR 346 ++ T SG+P+A AAGL +L++ Q G E A +AD EAA Sbjct: 312 DGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLENAQA----VADYFQEAAH 360 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 116 bits (291), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 93/318 (29%), Positives = 146/318 (45%), Gaps = 32/318 (10%) Query: 35 VGGTPRFI-TKAQGPYFWDAEERRYIDYIGSWGPAIVGHAHPKVVQAVQEAAARGLSFGA 93 V P + T+ G + D +RY+DY+ W +GHA P+ + A ++ L + Sbjct: 14 VTNRPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHA-PQCIADALAAQSKKLINPS 72 Query: 94 P---TEGEIDMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGATKRD---------KI 141 P E I++A+ + ++V +SG EA A++LAR K++ +I Sbjct: 73 PAFYNEPSIELAK-LLTANSVFDRVFFANSGGEANEGAIKLARKWGKKNPAADGSARFEI 131 Query: 142 VKFEGCYHGHADSLLVKAGS-GLLTFGNPTSSGVPEDFVKHTLVLDYNNTQQLEEAFKAA 200 + F+ +HG + + +G G T P G P+ + LE Sbjct: 132 ITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKAVL-----------NDLESVKALI 180 Query: 201 GNDIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYGAILIFDEVMSGF-RVALGGAQSL 259 G V++EPV G ++PA+ EF+Q +R L E +LI DEV SG R A Sbjct: 181 GEHTVAVMLEPVQGEGGVIPASKEFMQGLRSLTKEKNLLLIVDEVQSGMGRTGQLFAYQH 240 Query: 260 YDITPDLTALGKVIGGGLPVAAFGGRADIMRHLAPLGGVYQAGTLSGNPVAVAAGLTTLK 319 I PD+ L K IGGG+P+AA R +I A Q GT +GNP+ A G+ +K Sbjct: 241 SGIEPDIMTLAKGIGGGVPLAALLAREEI----ACFEAGEQGGTYNGNPLMTAVGVAVIK 296 Query: 320 IIQEAGFYEQLSAQTRKL 337 + + GF E + + + L Sbjct: 297 ELLKPGFMESVRERGQYL 314 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 112 bits (279), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 89/311 (28%), Positives = 148/311 (47%), Gaps = 24/311 (7%) Query: 33 RSVGGTPRFITKAQGPYFWDAEERRYIDYIGSWGPAIVGHAHPKVVQAVQEAAARGL-SF 91 R G P + +A G F D + +ID G + VG +P+VV+A+++ + + S Sbjct: 42 RGGGRMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSM 101 Query: 92 GAPTEGEIDMAEEICKLVPSIEQVRLVS----SGTEATMSALRLARGATKRDKIVKFEGC 147 + ++A +I +++P + ++ G++A +A++ A+ T R +I+ F G Sbjct: 102 EVNSSKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGG 161 Query: 148 YHG--HADSLLVKAGSGLLTFGNPTSSGVPEDFVKHTLVLDYNNTQQLEEAFK------- 198 YHG +A + L + FG P GV + ++ + + E Sbjct: 162 YHGIWNASNALTTGTAYRKGFG-PFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYVDYL 220 Query: 199 -----AAGNDIACVIVEPVAGNMNLVPATAEFLQTMRRLCTEYGAILIFDEVMSGF-RVA 252 A +D+A VIVEPV G VP + EFLQ +R+ C GA+LI DEV +G R Sbjct: 221 LNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTG 280 Query: 253 LGGAQSLYDITPDLTALGKVIGGGLPVAAFGGRADIMRHLAPLGGVYQAGTLSGNPVAVA 312 A + PD+ GK IGG +P+A R+D+ + P G Q T + N ++ A Sbjct: 281 KMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKI-PDGS--QPNTFAANSISAA 337 Query: 313 AGLTTLKIIQE 323 LT + I+Q+ Sbjct: 338 VALTNISILQD 348 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,430,367 Number of extensions: 64350 Number of successful extensions: 238 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 223 Number of HSP's successfully gapped: 9 Length of query: 433 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 345 Effective length of database: 1,203,190 Effective search space: 415100550 Effective search space used: 415100550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)