BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0031.p (665 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 371 e-103 gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 244 1e-65 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 192 6e-50 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 191 2e-49 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 175 1e-44 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 371 bits (953), Expect = e-103, Method: Compositional matrix adjust. Identities = 228/466 (48%), Positives = 293/466 (62%), Gaps = 24/466 (5%) Query: 24 APAQPGTAAPLIRIRVGILDSVLNQAGEISISRSGLESEVNILRQHLADLGSNVARLSTQ 83 AP P APLIRIR +LD +LNQ GE+SI+RS L++EV L+ LG + RLS + Sbjct: 284 APVLP---APLIRIRTTVLDKLLNQVGEVSIARSRLDNEVGSLQSESKALGEQLMRLSGE 340 Query: 84 LREVEIQAEIQIAASNQKQPQNQHFDPLEFDRFTHLHELTRMLAESVNDVASL-QKNLLD 142 LR+ Q+ Q H L L + ASL Q+ L + Sbjct: 341 LRQWLRQSGTQAQDGGDAA----------AAMQALAHSLAARLEQ-----ASLHQRRLQE 385 Query: 143 SVDRTQNGLVHQARLTRDMQRELMHARMVQFRGIEEPPHRLVRQMAKETGKELALDIVGG 202 V T+ GL QA TR++Q+ELM+ARMV+F +E LVRQ+A ETGK LALD+ Sbjct: 386 GVSSTREGLRQQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNC 445 Query: 203 AVELDRSILEKMVGPFEHLLRNAAAHGIEGAEQRLAAGKDAVGRLLLEVRQEGNEAMIRL 262 +++DR+ILE+++GP EHLLRNAA HGIE AEQRLA GK AVGRL ++ EGNEA+IR+ Sbjct: 446 RLQIDRAILERLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRV 505 Query: 263 SDDGQGLDLARIHEKALHLGLIDPHHRLSDAELTSIIFHPGFSTSSNVTAIAGRGVGMDV 322 SDDG+GLDL I +KAL LG S A L +IF PG STS V+A+AGRG+GMDV Sbjct: 506 SDDGRGLDLEAIRQKALELGAAG-LAEASQARLADLIFQPGLSTSPQVSALAGRGIGMDV 564 Query: 323 VRSEVASLGGRISIATIAGKGAQFIIHLPLSLAVAQVVLLELDQQSYAIPSLLVEQVLQI 382 VRSEVA+LGG + + + G+G QF ++LPLSLAV V LL QQ +AIPS+LV+ ++Q+ Sbjct: 565 VRSEVAALGGWMRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIVQL 624 Query: 383 KRADQDRVLREGLLEWQGRALPLHYLSALLGDERNIPQQQ---ALPVLVIKSGHDFLAIL 439 + + L E LE QG L LH LL DE P + + VL+IK +A+L Sbjct: 625 RGPQARQALAERSLEHQGSRLALHPWHCLL-DEAPEPAGEGSDSAYVLLIKRDDGLMAVL 683 Query: 440 VDRIVGNREVVTKSIGPQLTGMTGVVGATVLGSGSIVLILNPIQLA 485 D + GNREVV K +GPQL + GVVGATVLG G IVLI+NP+ LA Sbjct: 684 ADHVSGNREVVVKPVGPQLATLAGVVGATVLGDGQIVLIINPLLLA 729 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 244 bits (624), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 180/536 (33%), Positives = 283/536 (52%), Gaps = 25/536 (4%) Query: 105 NQHFDPLEFDRF--THLHELTRMLAESVNDVASLQKNLLDSVDRTQNGLVHQARLTRDMQ 162 Q + D F T EL R L + + Q + LD+ DR L++ + Sbjct: 247 QQKLATTQLDEFAQTAFAEL-RTLMQQNQQLLGEQLSALDAFDR------RSVNLSQRLY 299 Query: 163 RELMHARMVQFRGIEEPPHRLVRQMAKETGKELALDIVGGAVELDRSILEKMVGPFEHLL 222 E + RM F R+VR + GK + LDIVG ++DR ILEK+ P HLL Sbjct: 300 DEALACRMRPFADGTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLL 359 Query: 223 RNAAAHGIEGAEQRLAAGKDAVGRLLLEVRQEGNEAMIRLSDDGQGLDLARIHEKALHLG 282 RNA HGIE + QR AAGK G + LE R +I ++DDG G+D+ ++ + G Sbjct: 360 RNAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVVARG 419 Query: 283 LIDPH--HRLSDAELTSIIFHPGFSTSSNVTAIAGRGVGMDVVRSEVASLGGRISIATIA 340 L + RLS+AEL + P FS VT I+GRGVG+DVV + + G I I T A Sbjct: 420 LSNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQA 479 Query: 341 GKGAQFIIHLPLSLAVAQVVLLELDQQSYAIPSLLVEQVLQIKRADQDRVLREGLLEWQ- 399 G+G++F++ LPL+L+V + +L+++ + YA P V + L++ A+ + L EG + Sbjct: 480 GQGSRFLMQLPLTLSVIRSLLVDIAGEPYAFPLAYVNRTLRLP-AEALQTL-EGYQHFSH 537 Query: 400 -GRALPLHYLSALLGDERNIPQQQALPVLVIKSGHDFLAILVDRIVGNREVVTKSIGPQL 458 GR + L +L + + ++ V+VI + VD +G R +V + + P+L Sbjct: 538 GGRQVGLVSAHQVLQKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRL 597 Query: 459 TGMTGVVGATVLGSGSIVLILNPIQLAQRQGYQRMLRREQLQGAD-------GGVSRKIV 511 + V+ ++ G+ +LIL+ + + +++ +LQ D +RK V Sbjct: 598 GKIADVLAGALMEDGTPLLILDVADMLRT--VEKLTSSGRLQTVDQDGAAPTTTAARKKV 655 Query: 512 LVVDDSLTVRRVMHRLLTREGYQVVLATDGVDALHQMQAVRPDIVLLDIEMPRMDGFDLT 571 LVVDDSLTVR + +LLT GYQV +A DG+D + ++A D+V+ DI+MPRMDG +L Sbjct: 656 LVVDDSLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRADSFDLVITDIDMPRMDGIELV 715 Query: 572 RNIRDMAATATLPIIMITSRTAAKHRERAMELGVNAYLGK-PYQDDVLLNLIHGLI 626 IR A +LP+++++ + + R+R +E G + Y K + D+ LL + LI Sbjct: 716 SLIRGAANLQSLPVMIVSYKDREEDRQRGLEAGADHYFTKSSFHDESLLQAVTDLI 771 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 192 bits (489), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 112/329 (34%), Positives = 196/329 (59%), Gaps = 4/329 (1%) Query: 156 RLTRDMQRELMHARMVQFRGIEEPPHRLVRQMAKETGKELALDIVGGAVELDRSILEKMV 215 R+ +MQ +M RM+ + + RLVR ++++ GKE+ L +VG E D++I+E + Sbjct: 504 RIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALA 563 Query: 216 GPFEHLLRNAAAHGIEGAEQRLAAGKDAVGRLLLEVRQEGNEAMIRLSDDGQGLDLARIH 275 P H++RN+ HG+EG EQR A+GK A+G L + EG++ +I + DDG+G+D A I Sbjct: 564 DPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIK 623 Query: 276 EKALHLGLIDPHH--RLSDAELTSIIFHPGFSTSSNVTAIAGRGVGMDVVRSEVASLGGR 333 KA GLID R+SD + +++F GFST+ V+ ++GRGVGMDVVR+ V + G Sbjct: 624 RKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGS 683 Query: 334 ISIATIAGKGAQFIIHLPLSLAVAQVVLLELDQQSYAIPSLLVEQVLQIKRADQDRVLRE 393 +++ +++GKG + I LPLS+AV +V+++E D Q + +P V + +++ R+ Sbjct: 684 LALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVRVPRSSLHFANSS 743 Query: 394 GLLEWQGRALPLHYLSALLG--DERNIPQQQALPVLVIKSGHDFLAILVDRIVGNREVVT 451 + +G L L ++ +LG E+ + VLV++ G + +++D +V+ Sbjct: 744 AKMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQ 803 Query: 452 KSIGPQLTGMTGVVGATVLGSGSIVLILN 480 K + L G++ G+ ++G GS++++LN Sbjct: 804 KPLLGILGGLSLYSGSALMGDGSVLMVLN 832 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 191 bits (484), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 114/333 (34%), Positives = 185/333 (55%), Gaps = 2/333 (0%) Query: 149 NGLVHQARLTRDMQRELMHARMVQFRGIEEPPHRLVRQMAKETGKELALDIVGGAVELDR 208 N + +R TRD+Q +M RM+ + R+VR +A + GK++ G A ELD+ Sbjct: 348 NSVSQLSRNTRDLQESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDK 407 Query: 209 SILEKMVGPFEHLLRNAAAHGIEGAEQRLAAGKDAVGRLLLEVRQEGNEAMIRLSDDGQG 268 ++E++V P HL+RN+ HGIE + R A+GK G L L +G +I ++DDG G Sbjct: 408 GLIERIVDPLTHLVRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGG 467 Query: 269 LDLARIHEKALHLGLIDPHHRLSDAELTSIIFHPGFSTSSNVTAIAGRGVGMDVVRSEVA 328 L+ +I KA G+ LSDAE+ +IF PGFST+ VT ++GRGVGMDVV+ + Sbjct: 468 LNREKILAKAKQQGM-PVTDNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNIL 526 Query: 329 SLGGRISIATIAGKGAQFIIHLPLSLAVAQVVLLELDQQSYAIPSLLVEQVLQIKRADQD 388 ++GG + I + G G I LPL+LA+ + + + ++ Y +P V + LQ D Sbjct: 527 AMGGAVDIRSSKGFGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIK 586 Query: 389 RVLREG-LLEWQGRALPLHYLSALLGDERNIPQQQALPVLVIKSGHDFLAILVDRIVGNR 447 + +G +L +G LPL L + E + V++++S A+ VD ++G + Sbjct: 587 EISGQGQVLRVRGEYLPLIPLYQIFNIEPSFTDPSQGLVVILESDGKKAALFVDDLIGQQ 646 Query: 448 EVVTKSIGPQLTGMTGVVGATVLGSGSIVLILN 480 ++V K++ + G+ GAT+LG G + LI++ Sbjct: 647 QIVVKNLESNYRKVAGISGATILGDGGVALIVD 679 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 175 bits (443), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 128/391 (32%), Positives = 204/391 (52%), Gaps = 22/391 (5%) Query: 97 ASNQKQPQNQHFDPLEFDRFTHLHELTRML---AESVNDVASLQKNL-LDSVDRTQNGLV 152 A ++++ Q Q F +E + L +L L S +A +K+ + + + L+ Sbjct: 322 ALDERRAQEQKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDSQFEEATQAIDTLM 381 Query: 153 HQARLTRDMQRELMHARMVQFRGIEEPPHRLVRQMAKETGKELALDIVGGAVELDRSILE 212 Q RD L RMVQ + + R+VR +A++ GK++ L + G ELD+S++E Sbjct: 382 EQ---IRDAALTL---RMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVE 435 Query: 213 KMVGPFEHLLRNAAAHGIEGAEQRLAAGKDAVGRLLLEVRQEGNEAMIRLSDDGQGLDLA 272 ++ P H++RNA HGIE A +RLAAGK A L L E +I + DDG+G+D Sbjct: 436 RLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDRE 495 Query: 273 RIHEKALHLGLIDPHHRLSDAELTSIIFHPGFSTSSNVTAIAGRGVGMDVVRSEVASLGG 332 I KA+ GL P LSDAE+ +F PGFST+ VT ++GRGVGMDVVR + +L G Sbjct: 496 AILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHG 555 Query: 333 RISIATIAGKGAQFIIHLPLSLAVAQVVLLELDQQSYAIPSLLVEQVLQI---KRADQDR 389 +SI T G+G + LPL+LA+ + + + IP +V + + + + Sbjct: 556 EVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQPGSHIV 615 Query: 390 VLREGLLEWQGRALPLHYLSALLGDERNIPQQQALPVLVIKSGHDFLAILVDRIVGNREV 449 +R+ L + +PL L L ER +++++ G +LVD ++G + Sbjct: 616 NVRDEPLPF----VPLRELFDLPARERGRNS-----LVIVQYGPLRAGLLVDGLLGECQA 666 Query: 450 VTKSIGPQLTGMTGVVGATVLGSGSIVLILN 480 V K +G + G+ G+T+LG G + LIL+ Sbjct: 667 VIKPLGRLFGKVKGLSGSTILGDGRVALILD 697 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,998,922 Number of extensions: 88205 Number of successful extensions: 569 Number of sequences better than 1.0e-08: 57 Number of HSP's gapped: 538 Number of HSP's successfully gapped: 57 Length of query: 665 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 574 Effective length of database: 1,188,985 Effective search space: 682477390 Effective search space used: 682477390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)