BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0025.p (376 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxido... 692 0.0 gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase la... 73 3e-14 gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase... 64 2e-11 >gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 374 Score = 692 bits (1785), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/369 (87%), Positives = 346/369 (93%), Gaps = 3/369 (0%) Query: 5 MSDLATFAKLARSNAQLPVDVYFDDALFKREIHQLFQQGPRYAGHELMVPNVGDYATLPW 64 MSDLATFAKLARSNAQLPV VYFD+ L++RE+ QLFQQGPRY GHELMVP GDY+TLPW Sbjct: 1 MSDLATFAKLARSNAQLPVHVYFDEPLYQRELKQLFQQGPRYVGHELMVPEAGDYSTLPW 60 Query: 65 ENEGRMLVRNAHGIELMSNVCRHRQAKMLDGRGNARNIVCPLHRWTYDLKGDLIGAPHFG 124 ENEGRMLVRNA GIEL+SNVCRHRQAKMLDGRG+A NIVCPLHRWTYDLKG+LIGAPHFG Sbjct: 61 ENEGRMLVRNAQGIELVSNVCRHRQAKMLDGRGHASNIVCPLHRWTYDLKGELIGAPHFG 120 Query: 125 ETPCLNLSKTPLQNWNGLLFENNGKDGNDVAAQLKQLGVTRDLDFSGYLFDHVEVHECDY 184 +TPC+NLSKTPLQ+WNGLLFE NG D +A +LK LGVTRDLDFSGYL+DH EVHECDY Sbjct: 121 QTPCMNLSKTPLQSWNGLLFEANGVD---IADKLKNLGVTRDLDFSGYLYDHTEVHECDY 177 Query: 185 NWKTFIEVYLEDYHVEPFHPGLGSFVSCDDLKWEFGRNYSVQTVGVNHGLAKSGSPTYKK 244 NWKTFIEVYLEDYHVEPFHPGLG+FVSC DLKWEFGR+YSVQTVGVNHGL KSGSPTY K Sbjct: 178 NWKTFIEVYLEDYHVEPFHPGLGNFVSCSDLKWEFGRDYSVQTVGVNHGLIKSGSPTYAK 237 Query: 245 WQEQVLRFGNGQPPKYGAIWLTLYPNIMVEWYPHVLVVSTLWPNGPQKTTNVVEFYYPEE 304 WQE++L+F NG+PPK+GAIWLTLYPNIMVEWYPHVLVVSTLWP GPQKTTNVVEFYYPEE Sbjct: 238 WQEKLLQFSNGKPPKFGAIWLTLYPNIMVEWYPHVLVVSTLWPTGPQKTTNVVEFYYPEE 297 Query: 305 IALFEREMVEAERAAYMETCVEDDEIALRMDAGRRILMDRGTSEVGPYQSPMEDGMQHFH 364 IALFERE+VEAERAAYMETCVEDDEIALRMDAGRRILMDRGTSEVGPYQSPMEDGMQHFH Sbjct: 298 IALFERELVEAERAAYMETCVEDDEIALRMDAGRRILMDRGTSEVGPYQSPMEDGMQHFH 357 Query: 365 EWYRSQIAV 373 EWYR Q+ V Sbjct: 358 EWYRKQLEV 366 >gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase large terminal subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 73.2 bits (178), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 59/215 (27%), Positives = 103/215 (47%), Gaps = 14/215 (6%) Query: 9 ATFAKLARSNAQLPVDVYFDDALFKREIHQLFQQGPRYAGHELMVPNVGDYATLPWENEG 68 +++A L + +A + +Y D ALF+ E+ ++F + + HE + N GD+ T + Sbjct: 14 SSYADLVQ-DAVVDPSLYTDPALFEAEMDKIFYKTWIWVAHESEIKNPGDFKTAQIGRQP 72 Query: 69 RMLVRNAHG-IELMSNVCRHRQAKMLDG-RGNARNIVCPLHRWTYDLKGDLIGAPH-FGE 125 ++VR+ G I ++ N CRHR A + + +GNA CP H W+Y L G L G P+ G Sbjct: 73 VIVVRDKTGKINVLENRCRHRGATVCEKHKGNATGFTCPYHSWSYGLDGKLRGLPYPEGY 132 Query: 126 TPCLNLSKTPLQ-----NWNGLLFENNGKDGNDVAAQLKQLGVTRDL---DFSGYLFDHV 177 + + PLQ +++G++F + ++ ++ L DL +G+ Sbjct: 133 EDVIEKADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLFMKQGAGFPIKVQ 192 Query: 178 EVHECDY--NWKTFIEVYLEDYHVEPFHPGLGSFV 210 H+ + NWK +E + YH H S V Sbjct: 193 GEHKFSFKGNWKIQLENTTDGYHFPVVHKTFLSSV 227 >gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 462 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 70/294 (23%), Positives = 111/294 (37%), Gaps = 55/294 (18%) Query: 24 DVYFDDALFKREIHQLFQQGPRYAGHELMVPNVGDYATLPWENEGRMLVRNAHG-IELMS 82 D++ + LF+ E+ LF+ Y HE +P + DY T + ++ R+ G + + Sbjct: 35 DIFTNPDLFELEMKHLFESNWVYLAHESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVI 94 Query: 83 NVCRHRQAKMLDGR-GNARNIVCPLHRWTYDLKGDLIGAP---------HFGETPCLNLS 132 N C H+ A + + GN + CP H WT+ G L+ F + +L+ Sbjct: 95 NACSHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLT 154 Query: 133 KTP-LQNWNGLLFENNGKDGNDVAAQLKQLGVTRDL----------------DFSGYLFD 175 + Q++ G LF G DV + LG TR + S YL+D Sbjct: 155 RVARFQSYRGFLF---GSLSEDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSYLYD 211 Query: 176 HVEVHECDYNWKTFIEVYLEDYHVEPFHPGLGSFVSCDD---LKWEFGRNYSVQTVGV-- 230 NWK +E + YHV H + +S K N+S GV Sbjct: 212 G--------NWKMQMENGCDGYHVSSVHWNYAATMSRRKEGGTKATDANNWSKAVAGVYG 263 Query: 231 ---------NHGLAKSGSPTYKKWQEQVLRFGNGQPPKYGAIW--LTLYPNIMV 273 + L P + + +E R G + A L LYPN+ + Sbjct: 264 FENGHILLWTNTLNPEVRPIWNQREELAARVGQARADTIVAQTRNLCLYPNVFL 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,293,447 Number of extensions: 57126 Number of successful extensions: 115 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 113 Number of HSP's successfully gapped: 3 Length of query: 376 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 289 Effective length of database: 1,207,925 Effective search space: 349090325 Effective search space used: 349090325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)