BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0018.p (426 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s... 791 0.0 gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas... 187 2e-48 gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas... 123 2e-29 gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum ... 60 2e-10 >gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked subunit) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 791 bits (2043), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/415 (92%), Positives = 400/415 (96%) Query: 12 RRAGTGLSGGAMPIADGVVVSTAKFNKIVSMDKYSRTAVVQPGVRNLAISEAAAPHGLYY 71 R AGTGLSGGA PIADGVVVSTAKFN+I+ MDKYSRTAVVQPGVRNLAISEAAAPHGLYY Sbjct: 81 RGAGTGLSGGATPIADGVVVSTAKFNQILQMDKYSRTAVVQPGVRNLAISEAAAPHGLYY 140 Query: 72 APDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVLRVRVVTIEGDVIELGNAGLDAPGL 131 APDPSSQIAC+IGGNVAENSGGVHCLKYGLTVHNVLRVR+VTIEG+V+ELG+ LDAPGL Sbjct: 141 APDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNVLRVRMVTIEGEVVELGSGALDAPGL 200 Query: 132 DLLAVFIGSEGMLGIVTEVTVKLVPKPQQARVIMASFDDVVKGGNAVANVIAAGIIPAGL 191 DLLAVFIGSEGMLG+VTEVTVKLVPKPQ ARVIMASFDDVVKGGNAVANVIAAGIIPAGL Sbjct: 201 DLLAVFIGSEGMLGVVTEVTVKLVPKPQAARVIMASFDDVVKGGNAVANVIAAGIIPAGL 260 Query: 192 EMMDKTSSRMVEPFVKAGYDIDAEAILLCESDGTSEEVEEEIGRMSEVLNASGATAIAVS 251 EMMDKTSSRMVEPFVKAGYDIDAEAILLCESDGT EEVEEEIGRM++VLNASGATAIA S Sbjct: 261 EMMDKTSSRMVEPFVKAGYDIDAEAILLCESDGTVEEVEEEIGRMTDVLNASGATAIACS 320 Query: 252 QSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKRLAEVLLGIARMEGKYGLRCANV 311 QSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRK+LA+VLLGIA ME KYGLRCANV Sbjct: 321 QSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKKLAQVLLGIAEMEKKYGLRCANV 380 Query: 312 FHAGDGNLHPLILFDANQADEFHRAELFGAEILELCVEVGGTITGEHGVGIEKINSMCVQ 371 FHAGDGNLHPLILFDAN ADEFHRAELFGAEILELCVEVGGTITGEHGVGIEKINSMCVQ Sbjct: 381 FHAGDGNLHPLILFDANIADEFHRAELFGAEILELCVEVGGTITGEHGVGIEKINSMCVQ 440 Query: 372 YSPEEREAFFKVKRAFDTAFLLNPDKAIPSLHRCAEYGKMHVQRGAIKFPDLPRF 426 +SP ERE FFK+KRAFD AFLLNPDKAIP+LHRCAEYGKMHVQRG ++FPDLPRF Sbjct: 441 FSPAERETFFKLKRAFDPAFLLNPDKAIPTLHRCAEYGKMHVQRGQLRFPDLPRF 495 >gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 116/389 (29%), Positives = 194/389 (49%), Gaps = 7/389 (1%) Query: 14 AGTGLSGGAMPIADGVVVSTAKFNKIVSMDKYSRTAVVQPGVRNLAISEAAAPHGLYYAP 73 AGT L G + + GV V ++ N+++++ TA VQ GV +++ GL++ Sbjct: 83 AGTSLEGHVLALQGGVTVDLSQMNQVLAVHAEDLTATVQAGVTRKQLNQEIKDTGLFFPI 142 Query: 74 DPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVLRVRVVTIEGDVIELGN-AGLDAPGLD 132 DP + ++GG + + G + ++YG N L + VVT +G++I+ G A + G D Sbjct: 143 DPGAD--ASLGGMASTRASGTNAVRYGTMKENTLTLTVVTAQGEIIKTGTRAKKSSAGYD 200 Query: 133 LLAVFIGSEGMLGIVTEVTVKLVPKPQQARVIMASFDDVVKGGNAVANVIAAGIIPAGLE 192 L V++GSEG LGI+TEVTV+L P+P+ + SF V N V I G+ A +E Sbjct: 201 LTRVYVGSEGTLGIITEVTVRLYPQPEAISAAICSFPTVADAVNTVIQTIQMGVPLARVE 260 Query: 193 MMDKTSSRMVEPFVKAGYDIDAEAILLCESDGTSEEVEEEIGRMSEVLNASGATAIAVSQ 252 ++D+ R + K ++ +LL E G+ V+E+ + ++ A + Sbjct: 261 LLDENGVRAINAHDKLSLPVNP--LLLFEFHGSENGVKEQAELVQDIAKEFHALGFEWAT 318 Query: 253 SEAERLRFWSGRKNAFPAAGRISPDYYCM--DGTIPRKRLAEVLLGIARMEGKYGLRCAN 310 +R R W+ R NA+ A ++ P + D +P RLAE +L + GL A Sbjct: 319 RPEDRTRLWTARHNAYFALLQLRPGARAISTDCCVPISRLAECVLATKADCEEQGLTHAI 378 Query: 311 VFHAGDGNLHPLILFDANQADEFHRAELFGAEILELCVEVGGTITGEHGVGIEKINSMCV 370 + H GDGN H ++ D N + RAE ++ + + GT TGEHGVG+ K++ + Sbjct: 379 IGHVGDGNFHVQMMVDPNDPADIARAEGVNQRMVARAIGMDGTCTGEHGVGLHKMDFLIQ 438 Query: 371 QYSPEEREAFFKVKRAFDTAFLLNPDKAI 399 ++ +K A D ++NP K + Sbjct: 439 EHGEGAIAVMRAIKHALDPKNIMNPGKIL 467 >gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 112/398 (28%), Positives = 176/398 (44%), Gaps = 15/398 (3%) Query: 16 TGLSGGAMPIADG--VVVSTAKFNKIVSMDKYSRTAVVQPGVRNLAISEAAAPHGLYYAP 73 TGL G++P G VV+S + N+I ++D + T V+ G + E AA G + Sbjct: 79 TGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGCVLQHLQEQAAAAGRLFPL 138 Query: 74 DPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVLRVRVVTIEGDVI-ELGNAGLDAPGLD 132 +++ +CTIGGN++ N+GG L+YG T L + VVT +G+++ L D G D Sbjct: 139 SLAAEGSCTIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEIMSSLKGLRKDNTGYD 198 Query: 133 LLAVFIGSEGMLGIVTEVTVKLVPKPQ-QARVIMASFDDVVKGGNAVANVIAAGIIPAGL 191 L +FIG+EG LG++T +KL P+P+ Q + A G G Sbjct: 199 LRDLFIGAEGTLGVITAAVMKLFPQPRAQVTALAALPHPAQALQLLQLAQAHCGPALTGF 258 Query: 192 EMMDKTSSRMVE---PFVKAGYDI-DAEAILLCESDGTSEEVEEEI--GRMSEVLNASGA 245 E+M ++V P + +D + +LL SD SEE + G + L Sbjct: 259 ELMSDFCLQLVRKHFPEQRLPFDRPHPQYVLLELSDSESEEHARALFEGLIGTALEQGLV 318 Query: 246 TAIAVSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKRLAEVLLG--IARMEGK 303 ++ S A+ W R++ A + D ++P R+AE + + + Sbjct: 319 DDAVIAASLAQSRALWRLRESISSAQAHEGKNIK-HDISVPISRIAEFIAATDVLVQQAA 377 Query: 304 YGLRCANVFHAGDGNLHPLILFDANQADE--FHRAELFGAEILELCVEVGGTITGEHGVG 361 G R H GDGNLH + AD R + + GG+I+ EHG+G Sbjct: 378 PGCRMVTFGHLGDGNLHYNVSPPEGVADRDFIARQAAINRVVHDSVDRFGGSISAEHGLG 437 Query: 362 IEKINSMCVQYSPEEREAFFKVKRAFDTAFLLNPDKAI 399 K + SP E + +K A D L+NP K + Sbjct: 438 ALKREEITRYKSPVELQLMRAIKLALDPQQLMNPGKVL 475 >gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 1333 Score = 60.5 bits (145), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 82/322 (25%), Positives = 121/322 (37%), Gaps = 67/322 (20%) Query: 42 MDKYSRTAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGL 101 +DK T GV +S+AA G +A DP+S A IGGNVA N+GG + +G Sbjct: 263 LDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAEASCIGGNVAMNAGGKKAVLWGT 322 Query: 102 TVHNVLRVRVVTIEGDVIEL-------------------------GNAGLDAP------- 129 + N+ R+V +GD +E+ G G Sbjct: 323 ALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVARFKLEWSHPGEKGQKTEVFKTEIL 382 Query: 130 ------------GLDLLAVFIG------SEGMLGIVTEVTVKLVPKPQQARVIMASF--- 168 G D+ F+ EG G++T L P+Q R + F Sbjct: 383 EISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLITSARWILHKMPKQTRTVCLEFFGQ 442 Query: 169 -DDVVKGGNAVANVIAA-----GIIPAGLEMMDKTSSRMVEPFVKAGYDIDAEAILLCES 222 D + + + + A G I AGLE +D+ R V K+ + + +L+ + Sbjct: 443 ARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYLRAVGYATKSKRGVLPKMVLIGDI 502 Query: 223 DGTSEE----VEEEIGRMSEVLNASGATAIAVSQSEAERLRFWSGRKNAFPAAGRISPDY 278 G E E+ RM+ N G +AVS EA R +FW R A + Sbjct: 503 VGDDENAVAAAASEVIRMAN--NRVGEGFVAVS-PEA-RKKFWLDRSRTAAIAKHTNAFK 558 Query: 279 YCMDGTIPRKRLAEVLLGIARM 300 D IP R+ E GI R+ Sbjct: 559 INEDVVIPLNRMGEYTDGIERI 580 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,211,205 Number of extensions: 45017 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 4 Length of query: 426 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 338 Effective length of database: 1,203,190 Effective search space: 406678220 Effective search space used: 406678220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)