BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0013.p (176 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 333 7e-93 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 117 6e-28 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 108 2e-25 gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 104 5e-24 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 103 6e-24 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 333 bits (853), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 152/176 (86%), Positives = 169/176 (96%) Query: 1 MEIAILDDSCKKLKPFETGEICVRGPAVFMGYHNNPDANAKAFKGDWFHTGDLGHVDQDG 60 M+IAIL+D ++L PFETGEICVRGPAVFMGYHNNP+ANAKAFK DWFHTGDLGHVDQDG Sbjct: 381 MQIAILNDDGQELAPFETGEICVRGPAVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDG 440 Query: 61 FLYITGRSSDMYISGGSNVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVAKSG 120 FLYITGRSSDMYISGGSNVYPREIEEALLTHP+VSEVAVLGVPDPKWGESG+AVIV K+G Sbjct: 441 FLYITGRSSDMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAG 500 Query: 121 QQADGDALLSHLESRIAKYKWPRRFVFWETMPKSGYGKIVKKQIKSLLEEKGDYRL 176 QQ DG+ALL+HLESRI KYKWPRRF++W++MPKSGYGKIVKKQIK+LLEE+G+YRL Sbjct: 501 QQVDGEALLAHLESRIGKYKWPRRFIYWDSMPKSGYGKIVKKQIKALLEEQGEYRL 556 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 117 bits (292), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 60/165 (36%), Positives = 92/165 (55%), Gaps = 2/165 (1%) Query: 2 EIAILDDSCKKLKPFETGEICVRGPAVFMGYHNNPDANAKAFKGD-WFHTGDLGHVDQDG 60 E AILDD + + + GEI +RGP V GY PD AK D +F TGD+G +D+ G Sbjct: 391 EFAILDDDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKVMTPDGFFKTGDIGVMDERG 450 Query: 61 FLYITGRSSDMYISGGSNVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVAKSG 120 + I R DM + G NVYP E+E + HP V E A +GVPD GE+ + + V + Sbjct: 451 YTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEA-VKLFVVRKD 509 Query: 121 QQADGDALLSHLESRIAKYKWPRRFVFWETMPKSGYGKIVKKQIK 165 + L+ + + + YK P+ F + +PK+ GKI++++++ Sbjct: 510 KTLSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 108 bits (271), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 60/153 (39%), Positives = 87/153 (56%), Gaps = 1/153 (0%) Query: 17 ETGEICVRGPAVFMGYHNNPDANAKAFKGDWFHTGDLGHVDQDGFLYITGRSSDMYISGG 76 E GEI RG GY N A +AF G WFHTGDLG ++ DG++ I RS D+ ISGG Sbjct: 388 EIGEIMFRGNICMKGYLKNDRATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIIISGG 447 Query: 77 SNVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVAKSGQQADGDALLSHLESRI 136 N+ E+E+ L HP+V AV+ PD KWGE+ A + K G + L S + Sbjct: 448 ENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKEGAEVGAAELTEFCRSHL 507 Query: 137 AKYKWPRRFVFWETMPKSGYGKIVKKQIKSLLE 169 A +K P+ +++ +PK+ GKI K +++ ++ Sbjct: 508 AGFKVPKA-IYFGPLPKTSTGKIQKFELRKRMK 539 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 104 bits (259), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 53/154 (34%), Positives = 90/154 (58%), Gaps = 6/154 (3%) Query: 18 TGEICVRGPAVFMGYHNNPDANAKAFKGDWFHTGDLGHVDQDGFLYITGRSSDMYISGGS 77 TGE+ VR P + GY + +A+ + ++G W HT D+GH+D +G+L +T R D+ +GG Sbjct: 386 TGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTGGE 445 Query: 78 NVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVAKSGQQA-DGDALLSHLES-- 134 V +IE+ + HP+V+E AV+G+ D KWGE +A++V K A D + H+ + Sbjct: 446 WVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPVALVVLKKDAAAVSEDDIREHVRAAA 505 Query: 135 ---RIAKYKWPRRFVFWETMPKSGYGKIVKKQIK 165 I++Y P R F + ++ GK+ K+ ++ Sbjct: 506 EAGAISRYGVPDRVRFVAELARTSVGKLNKRVMR 539 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 103 bits (258), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 57/170 (33%), Positives = 91/170 (53%), Gaps = 5/170 (2%) Query: 3 IAILDDSCKKLKPFETGEICVRGP--AVFMGYHNNPDANAKAFKGDWFHTGDLGHVDQDG 60 + I+D+ + L G I +R P +F+ Y N +A + F GD+ TGDLG +D+ G Sbjct: 378 VQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRNEEATREKFAGDFLLTGDLGSMDEQG 437 Query: 61 FLYITGRSSDMYISGGSNVYPREIEEALLTHPSVSEVAVLGVPDPKWGESGIAVIVAKSG 120 ++ GR+ D+ S G + P IEE L+ HP+V AV+GV D E A +V K G Sbjct: 438 YIRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDG 497 Query: 121 QQAD---GDALLSHLESRIAKYKWPRRFVFWETMPKSGYGKIVKKQIKSL 167 D L H+ +++A +++PR F +P + GKI++K +K + Sbjct: 498 VTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKEM 547 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 592,777 Number of extensions: 24143 Number of successful extensions: 94 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 80 Number of HSP's successfully gapped: 14 Length of query: 176 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 97 Effective length of database: 1,245,805 Effective search space: 120843085 Effective search space used: 120843085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)