BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0023.0012.p
         (364 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas...   389   e-109
gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li...    65   1e-11
gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H...    55   8e-09

>gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 556

 Score =  389 bits (998), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 189/236 (80%), Positives = 206/236 (87%), Gaps = 2/236 (0%)

Query: 1   MNLGRLLSDVARRFPDEPGLIFGDGTADGKGDKIATWKQINDRVDTLAHSLQKLGVSKGD 60
           MNLGRLLSDVARRFPDEPGLI   G ADG  D++  W++IN RVD LAH+L + GV KGD
Sbjct: 30  MNLGRLLSDVARRFPDEPGLILAQGAADGS-DRLIPWREINARVDALAHALTRRGVKKGD 88

Query: 61  KMLVHSRNNLQIFESAWAAFKLGMVWVPTNVRITPPEAAYLGQSSGASVMLYDRGFANYV 120
           KMLVHSRNN QIFESAWAAFK+G+VWVPTN RITPPEAAYLGQSSGA VMLYDRGFA YV
Sbjct: 89  KMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPPEAAYLGQSSGACVMLYDRGFAQYV 148

Query: 121 DAVKEVSPALKHVIALQDPRAGELDFETLATATGAHQSFDEVEVDYEDPLWFFYTSGTTG 180
           DAVK  SPAL+HV+AL D RAGELD+E L    G+  +F E EVDY+DPLWFFYTSGTTG
Sbjct: 149 DAVKAASPALEHVVALADARAGELDYEALVR-EGSGAAFREAEVDYDDPLWFFYTSGTTG 207

Query: 181 HPKAGMLSHGQMAFVVTNHLADLLPGLTHQSRSLVVAPLSHGAGIHAVVNTARGAA 236
           HPKAGMLSHGQMAFVVTNHLADL+PGLTHQSRSLVVAPLSHGAG+HAV+NTARGAA
Sbjct: 208 HPKAGMLSHGQMAFVVTNHLADLMPGLTHQSRSLVVAPLSHGAGVHAVINTARGAA 263


>gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid
           ligase II protein [Herbaspirillum seropedicae SmR1]
          Length = 546

 Score = 64.7 bits (156), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 61/195 (31%), Positives = 85/195 (43%), Gaps = 12/195 (6%)

Query: 21  IFGDGTADGKGDKIATWKQINDRVDTLAHSLQKLGVSKGDKMLVHSRNNLQIFESAWAAF 80
           ++G  TA   G     W Q   R   LA +LQ LGV K D +     N   + E+ +   
Sbjct: 30  VYGKRTAIIHGALRQDWDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAHFGVP 89

Query: 81  KLGMVWVPTNVRITPPEAAYLGQSSGASVMLYDRGFANYVDAVKEVSPALKHVIALQD-- 138
             G V    N+R+      ++ +   A V+L D  FA     ++   PALK V    +  
Sbjct: 90  MAGAVLNALNIRLDAESIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALKIVEVFDELG 149

Query: 139 --PRAGE----LDFETLATATGAHQSFD-EVEVDYEDPLWFFYTSGTTGHPKAGMLSH-G 190
             P AGE    L++E L    G  + FD ++  D  D +   YTSGTTG PK  +  H G
Sbjct: 150 PPPVAGERFGHLEYEAL--LAGGDEHFDWQMPADEWDAIALNYTSGTTGDPKGVVYHHRG 207

Query: 191 QMAFVVTNHLADLLP 205
                V+N L   LP
Sbjct: 208 AALNAVSNILEWDLP 222


>gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase
           [Herbaspirillum seropedicae SmR1]
          Length = 549

 Score = 55.5 bits (132), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 44/157 (28%), Positives = 70/157 (44%), Gaps = 6/157 (3%)

Query: 36  TWKQINDRVDTLAHSLQKLGVSKGDKMLVHSRNNLQIFESAWAAFKLGMVWVPTNVRITP 95
           ++ Q+    D  A++L+  GV +GD++ ++    ++   +  AA+KLG + VP      P
Sbjct: 64  SYDQLKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGP 123

Query: 96  PEAAYLGQSSGASVMLYDRGFANYVDAVKEVSPALKHVIALQDPRA---GELDFETLATA 152
              AY   +SGA  ++ D           E+ PAL+ V  ++          DF     A
Sbjct: 124 DAIAYRLDNSGAVALVTDASGMEKATLAGEL-PALRLVFCVESSDLVLPQTTDFWDRLHA 182

Query: 153 TGAHQSFDEVEVDYEDPLWFFYTSGTTGHPKAGMLSH 189
             A    D V    +DP    YTSGTTG  K  + +H
Sbjct: 183 APAE--LDPVLTLADDPAMIIYTSGTTGKAKGALHAH 217


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.135    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,288,223
Number of extensions: 58491
Number of successful extensions: 160
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 159
Number of HSP's successfully gapped: 4
Length of query: 364
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 278
Effective length of database: 1,212,660
Effective search space: 337119480
Effective search space used: 337119480
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)