BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0046.p (276 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 375 e-105 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 242 3e-65 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 240 7e-65 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 115 4e-27 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 114 9e-27 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 375 bits (964), Expect = e-105, Method: Compositional matrix adjust. Identities = 189/254 (74%), Positives = 213/254 (83%), Gaps = 8/254 (3%) Query: 1 VVDDAIVVLENISRHIEQGMKPVKAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVGR 60 VVDDAIVVLENISRHIE GMKP KAAL+GAREVGFTVLSMSISLIAVFIPILMM GIVGR Sbjct: 399 VVDDAIVVLENISRHIESGMKPYKAALLGAREVGFTVLSMSISLIAVFIPILMMEGIVGR 458 Query: 61 LFREFAVTLSVAILVSLLVSLTTTPMMCAMLLKQE--SEEERKPGRF------FTAVERL 112 LFREFA+TLSVAILVSL+VSLTTTPMMCA LL+ + ++E R+ R + Sbjct: 459 LFREFAITLSVAILVSLVVSLTTTPMMCAQLLRHDPAADEARRQRRHPWLQKIADQSAAI 518 Query: 113 FDSMLRGYERSLAWALRFSPLMIVILLATIALNVYLYVIVPKGFFPQQDTGRLVGGIRGD 172 F+ ML Y+R+LAWALR +PLM++ILLATIALNVYLY +PKGFFPQQDTGRLVGGIRGD Sbjct: 519 FEDMLLAYQRTLAWALRAAPLMMLILLATIALNVYLYKAIPKGFFPQQDTGRLVGGIRGD 578 Query: 173 QSISFQAMRIKLNEFIEIVRQDPAVANVTAFTGGGRRNGGSMFIALKPLSERKISADKVI 232 Q+ISFQAMR KLNEFI+IVR DPAV++V AFTGG RRNGG M++ LKPLSERK SADKV+ Sbjct: 579 QAISFQAMRTKLNEFIDIVRADPAVSDVVAFTGGQRRNGGFMYVTLKPLSERKDSADKVV 638 Query: 233 ARLRMKLGHVAXCR 246 ARLR+K VA R Sbjct: 639 ARLRIKTAKVAGAR 652 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 242 bits (617), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 127/244 (52%), Positives = 168/244 (68%), Gaps = 6/244 (2%) Query: 1 VVDDAIVVLENISRHIEQGMKPVKAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVGR 60 VVDDAIV++ENISR+IE+GMKP++AAL GA ++GFT++S++ SLIAV IP+L MG +VGR Sbjct: 399 VVDDAIVMIENISRYIEEGMKPMQAALKGAEQIGFTIISLTFSLIAVLIPLLFMGDVVGR 458 Query: 61 LFREFAVTLSVAILVSLLVSLTTTPMMCAMLLKQESEEERKPGRFFTAVERLFDSMLRGY 120 LF EFAVTL+V+IL+S +VSLT TPMMCA LL+ + EE++ G F+ ++ FD ++ Y Sbjct: 459 LFHEFAVTLAVSILISAVVSLTLTPMMCARLLRHQPEEQQ--GWFYHKSQQFFDRIIARY 516 Query: 121 ERSLAWALRFSPLMIVILLATIALNVYLYVIVPKGFFPQQDTGRLVGGIRGDQSISFQAM 180 L W L L +++ AT+ L V LYV VPKGFFP QDTG + G QSISF AM Sbjct: 517 GVMLQWVLARQKLTLLVAAATLGLTVLLYVFVPKGFFPLQDTGVIQGISEATQSISFSAM 576 Query: 181 RIKLNEFIEIVRQDPAVANVTAFTG----GGRRNGGSMFIALKPLSERKISADKVIARLR 236 K + ++V QDPAV ++T+F G N G M I LKP R++SA VI RL+ Sbjct: 577 GEKQQQLAKVVLQDPAVESLTSFIGVDGTNATLNSGRMLINLKPHDVREVSATDVIRRLQ 636 Query: 237 MKLG 240 KL Sbjct: 637 GKLA 640 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 240 bits (613), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 121/243 (49%), Positives = 170/243 (69%), Gaps = 3/243 (1%) Query: 1 VVDDAIVVLENISRHIEQGMKPVKAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVGR 60 VVDDAIV+LENI R++E+GM P++AAL GA E+GFT++S+S+SL+AVFIP+L+MGGIVGR Sbjct: 411 VVDDAIVMLENIYRYVEEGMTPLEAALKGAGEIGFTIISISVSLVAVFIPLLLMGGIVGR 470 Query: 61 LFREFAVTLSVAILVSLLVSLTTTPMMCAMLLKQESEEERKPGRFFTAVERLFDSMLRGY 120 LFREFAVT+++ I VS+++SLT TPM+C+ LK +E ++ G+ + ER FD+ML GY Sbjct: 471 LFREFAVTVTLTIAVSVIISLTLTPMLCSRFLK--NEHGQRHGKLYQWFERCFDAMLAGY 528 Query: 121 ERSLAWALRFSPLMIVILLATIALNVYLYVIVPKGFFPQQDTGRLVGGIRGDQSISFQAM 180 +R L ++ + ++ +AT+AL L++ +PKGFFPQQD G +VG Q S AM Sbjct: 529 KRGLDVVMKHQFITLMSFIATLALTAVLFIFIPKGFFPQQDNGVIVGFAESAQDTSSAAM 588 Query: 181 RIKLNEFIEIVRQDPAVANVTAFTGGGRRNGGSMFIALKPLSE-RKISADKVIARLRMKL 239 +L + E+VRQDP V G N G FIALKP + R +AD++IARLR K+ Sbjct: 589 HERLLKVAEVVRQDPDVIGFAMSAGSTTFNTGQFFIALKPKDQGRTTNADEIIARLRPKV 648 Query: 240 GHV 242 + Sbjct: 649 ARI 651 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 115 bits (288), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 73/243 (30%), Positives = 128/243 (52%), Gaps = 10/243 (4%) Query: 1 VVDDAIVVLENISRHI-EQGMKPVKAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVG 59 +VDDAIVV+EN+ R + E+G+ P A ++ ++ +++ LIAVFIP+ GG VG Sbjct: 405 LVDDAIVVVENVERIMSEEGLSPADATRKAMGQISGALIGITLVLIAVFIPMAFFGGAVG 464 Query: 60 RLFREFAVTLSVAILVSLLVSLTTTPMMCAMLLK--QESEEERKPGRFFTAVERLFDSML 117 ++R+F++++ +++ S+L++ T TP +CA +LK ++ K G FF R F+S Sbjct: 465 AIYRQFSLSMVASMVFSVLMAFTLTPALCATILKPVEKGHHHEKKG-FFGWFNRKFNSTA 523 Query: 118 RGYERSLAWALRFSPLMIVILLATIALNVYLYVIVPKGFFPQQDTGRLVGGIRGDQSISF 177 GY+ + L + +++ + +A LYV +P F P +D G ++ ++ S Sbjct: 524 SGYQGIIGGMLNKAGRYMIVYVLIVACVGLLYVRLPASFLPNEDQGYIITNVQLPPGAST 583 Query: 178 QAMRIKLNEFIEIVRQDPAVANVTAFTG----GGRRNGGSMFIALKPLSERK--ISADKV 231 + + E +DPAV + A G G +N +F LK S+R SAD + Sbjct: 584 SRTTAVVKQIEEYYLKDPAVEHAIAVLGYSFSGNGQNAALVFTPLKDWSQRDKSQSADAI 643 Query: 232 IAR 234 + Sbjct: 644 AGK 646 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 114 bits (285), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 84/273 (30%), Positives = 140/273 (51%), Gaps = 13/273 (4%) Query: 1 VVDDAIVVLENISRHI-EQGMKPVKAALIGAREVGFTVLSMSISLIAVFIPILMMGGIVG 59 +VDDAIVV+EN+ R + E+ + P +A +E+ ++ +++ L AVFIP+ GG G Sbjct: 407 LVDDAIVVVENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTG 466 Query: 60 RLFREFAVTLSVAILVSLLVSLTTTPMMCAMLLKQESEEERKPGRFFTAVERLFDSMLRG 119 ++R+F++T+ A+ +S++V+LT TP +CA LLK + + GRFFT R FD Sbjct: 467 VIYRQFSITIVSAMALSVMVALTLTPALCATLLKPHAGGHVQQGRFFTWFNRSFDRSATR 526 Query: 120 YERSLAWALRFSP--LMIVILLATIALNVYLYVIVPKGFFPQQDTGRLVGGIRGDQSISF 177 Y+ +A L+ L++ L+A +A+ V L++ +P F P++D G L+ I+ + Sbjct: 527 YQGGVAGLLKRGKRGLLMYALIA-VAMAV-LFMRLPTSFLPEEDQGVLMAQIQLPTGATA 584 Query: 178 QAMRIKLNEFIEIVRQDP-----AVANVTAFTGGGRRNGGSMFIALKPLSERK---ISAD 229 + + E P +A V A GG +NG FI LK +R SA Sbjct: 585 EQTATVVKTVQEYFMNKPESVESVLAIVGAGVGGNSQNGARAFIRLKDWEQRTGAGQSAS 644 Query: 230 KVIARLRMKLGHVAXCRYISIRPSLTRRTSRRS 262 +++ R L + R + P R + S Sbjct: 645 ELVRRANADLSKIRGARVFLMNPPAVRGLGQSS 677 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 693,951 Number of extensions: 24748 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 10 Length of query: 276 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 192 Effective length of database: 1,222,130 Effective search space: 234648960 Effective search space used: 234648960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)