BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0044.p (216 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 114 9e-27 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 102 2e-23 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 96 3e-21 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 93 2e-20 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 92 4e-20 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 114 bits (284), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 70/195 (35%), Positives = 111/195 (56%), Gaps = 5/195 (2%) Query: 25 GFTVDLADPQSIDACAKASVQWLGGLDGLVNNAAITNSGGKTTEQLAIDMWDKVMDVNVR 84 G D+ D S + A A +Q G +D LVNNA IT KT E D +D+++DV++R Sbjct: 57 GLVADVTDKASCETAAAAILQQFGRIDILVNNAGITQPV-KTLEITGAD-YDRILDVSLR 114 Query: 85 GTWLMTNACLPALRESGRGSVVNLSSDTPLWGATNLLA--YVASKSAVIGMTRSLARELG 142 GT M+ A LPA+R GS++ +SS + G L Y A+K+ V+G+ R++ARE G Sbjct: 115 GTLYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFG 174 Query: 143 GDNITVNAVAPGLTLVEATE-YVPKERHKTYHDRRAIQRDQLPEDVCGAVIFALSDLSRF 201 + I VN++ PGL + + + +E+ + + R +D+ GA +F SDLS++ Sbjct: 175 PEGIRVNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQY 234 Query: 202 YTGQVMAVNGGFVMN 216 TG + VNGG +++ Sbjct: 235 CTGITLDVNGGMLIH 249 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 102 bits (254), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 68/195 (34%), Positives = 107/195 (54%), Gaps = 5/195 (2%) Query: 25 GFTVDLADPQSIDACAKASVQWLGGLDGLVNNAAITNSGGKTTEQLAIDMWDKVMDVNVR 84 G D+ D + +A A A ++ G +D LVNNA +T K E D +D+++DV++R Sbjct: 57 GLVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPA-KLLEITGKD-YDRILDVSLR 114 Query: 85 GTWLMTNACLPALRESGRGSVVNLSSDTPLWGATNLLA--YVASKSAVIGMTRSLARELG 142 GT M+ A LPA++ GS+V +SS + G L Y A+K+ V+G+ R++ARE G Sbjct: 115 GTLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFG 174 Query: 143 GDNITVNAVAPGLTLVEA-TEYVPKERHKTYHDRRAIQRDQLPEDVCGAVIFALSDLSRF 201 I VN + PGL + + + E+ + R P+DV G +F SDLS++ Sbjct: 175 PQGIRVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQY 234 Query: 202 YTGQVMAVNGGFVMN 216 TG + VNGG +++ Sbjct: 235 CTGVTLDVNGGMLIH 249 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 95.9 bits (237), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 61/191 (31%), Positives = 103/191 (53%), Gaps = 6/191 (3%) Query: 29 DLADPQSIDACAKASVQWLGGLDGLVNNA-AITNSGGKTTEQLAIDMWDKVMDVNVRGTW 87 D+ D +I+A +V +G +D L+NNA A+ E + +++D ++++N R Sbjct: 64 DVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRA--PLETVTDELFDDIININARSVV 121 Query: 88 LMTNACLPALRESGRGSVVNLSSDTPLWGA-TNLLAYVASKSAVIGMTRSLARELGGDNI 146 T A +P +R G G+++N++S G L Y ASK V +TR +A+EL D I Sbjct: 122 AFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKI 181 Query: 147 TVNAVAPGLTLVEATE-YVPKERHKTYHDRRAIQRDQLPEDVCGAVIFALSD-LSRFYTG 204 VNAVAPG+ + E + E+ + + + R P++ GA ++ S+ +S + TG Sbjct: 182 RVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTG 241 Query: 205 QVMAVNGGFVM 215 Q++ VNGG M Sbjct: 242 QIIDVNGGQYM 252 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 92.8 bits (229), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 68/215 (31%), Positives = 106/215 (49%), Gaps = 3/215 (1%) Query: 1 MADILQDTLQGAVAGLQQRGLNVAGFTVDLADPQSIDACAKASVQWLGGLDGLVNNAAIT 60 +ADI D L G A +DL P+SI A + G D LVNNA + Sbjct: 37 VADINLDAASKTADALVAAGHRAAPLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVA 96 Query: 61 NSGGKTTEQLAIDMWDKVMDVNVRGTWLMTNACLPALRESGRGSVVNLSSDTPLWGATNL 120 + + ++ W + M+VNV G L + + G +VN+SS + + Sbjct: 97 RT--YSFLDYPLENWLQTMNVNVTGVLLAGQHAARLMVKQQWGRIVNISSISGIRAGAGR 154 Query: 121 LAYVASKSAVIGMTRSLARELGGDNITVNAVAPGLTLVEATEYVPKER-HKTYHDRRAIQ 179 AY SK+AVIG+TR +A EL ITVN+VAPG T+ + E+ ++Y+ ++ Sbjct: 155 TAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPMR 214 Query: 180 RDQLPEDVCGAVIFALSDLSRFYTGQVMAVNGGFV 214 R +++ AV + ++ S + TG V+ V+GGFV Sbjct: 215 RYGTTDEIASAVCYLAAEESAYITGHVIPVDGGFV 249 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 91.7 bits (226), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 71/225 (31%), Positives = 114/225 (50%), Gaps = 19/225 (8%) Query: 1 MADILQDTLQGAVAGLQQRGLNVAGFTVDLADPQSIDAC-AKASVQWLGGLDGLVNNAAI 59 + D+ +D L A A ++ + D++ + +DA A + +W G LD L+NNA I Sbjct: 36 ICDVSEDFLASARARFGHAPVSYS--RTDVSSEREVDAMFADLAQRWSGRLDVLINNAGI 93 Query: 60 TNSGGKTT--EQLAIDMWDKVMDVNVRGTWLMTNACLPALRESGRGSVVNLSSDTPLWGA 117 G T+ E++A+ WD+ + VN+ G +L T +P L+++G G++VN+SS G Sbjct: 94 A---GPTSPVEEVALSDWDQTLAVNLTGPFLCTRRAVPLLKKNGGGAIVNISSVAGRLGF 150 Query: 118 TNLLAYVASKSAVIGMTRSLARELGGDNITVNAVAPGLTLVEATEYVPKERHKTY---HD 174 Y ASK VIG+T + A ELG +I VNAV PG+ E + + Y H+ Sbjct: 151 ALRTPYSASKYGVIGLTETWAIELGPSHIRVNAVLPGIVEGARQERIVAAKAAAYGIGHE 210 Query: 175 --------RRAIQRDQLPEDVCGAVIFALSDLSRFYTGQVMAVNG 211 R ++++ ED+ VIF S +GQ ++V G Sbjct: 211 EMRQRLLSRVSLRKMVTAEDIANQVIFLCSPAGASISGQSLSVCG 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 674,961 Number of extensions: 26209 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 41 Length of query: 216 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 135 Effective length of database: 1,236,335 Effective search space: 166905225 Effective search space used: 166905225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)