BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0023.p (544 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 132 5e-32 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 110 2e-25 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 106 4e-24 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 105 7e-24 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 101 1e-22 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 132 bits (333), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 150/524 (28%), Positives = 220/524 (41%), Gaps = 99/524 (18%) Query: 10 DEVQQTIAAARTASLAWRNVPAPVRGELVRLLGEVLREKREALGLLVSLEAGKIRAEGLG 69 D+V ++ A A AW +P R ++ R + + + L +V+ E G + +G Sbjct: 48 DDVSASVELAIAAQAAWVAMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEH 107 Query: 70 EVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVITAFNFPVAVWAWNA 129 EV+E I +C A G+ Q G + S PG + PLGV GVI+ FNFP+ + Sbjct: 108 EVREAITLCHLAAGMPLQAQGQLLPST-PGRLSIARRAPLGVIGVISPFNFPLILTLRTV 166 Query: 130 ALALVCGNAVVWKPSEKTPVTALAVQALMRQAIARFGQQRPGAVPAGLCGLLIGGANVGA 189 A AL GNAVV KP +TPV+ M +AR +Q +PAGL +L GGA G Sbjct: 167 APALAAGNAVVVKPDLRTPVSG----GFM---LARIFEQ--AGLPAGLLHVLPGGAETGE 217 Query: 190 AISASPDVALVSATGSVAMGRKVAVTVARRLGRSLLELGGNNAMIVAPTADLNLALRAIV 249 A+ +P V +++ TGS A+GR++ R L + LELGG NA+++ ADL++A Sbjct: 218 ALVTAPGVPMIAFTGSPAVGRRIGELAGRHLKKLSLELGGANALVILEDADLDVAASHAA 277 Query: 250 FSAVGTAXLGRRRRRPSRHLPAATAGSTFGPEPRGLHRARYSRSLRR--RHAHVAQGLGH 307 + A LH+ + + R HA + +GL Sbjct: 278 WGA-------------------------------WLHQGQICMAANRILVHASLEEGLLQ 306 Query: 308 ATGPGPSSDLVGLXATGP---GPSSDLVGLRRVFIHRSLFDKVCADIERVYAGIRVGDPL 364 VG A+G GP D L+RV H + + V A + Sbjct: 307 RLADKARHLPVGDGASGQVALGPMIDERQLQRV--HALVQESVAAGAQ------------ 352 Query: 365 APETLVGPLIDGGAYDQMQQALAQAQKEGGNVLGGERVTTFGGEHAYYVRPALVRMPRQS 424 L+ GG Y+Q+ Q VLGG VRP + Sbjct: 353 --------LVAGGRYEQL---FYQP-----TVLGG-------------VRPGM------- 376 Query: 425 AVMHRETFAPILYAVAYDDVEEAIALNNAVPQGLSSAIFTNDVRESELFMSAQGSDCGLA 484 V E F P+ + +D+ E+AIAL N GL++ + + V + A G+ Sbjct: 377 RVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRA--MAVAARLRQGMV 434 Query: 485 NVNIGTSGAEIGGAFGGEKETGGGRESGSDA-WKNYMRRATNTI 527 +VN T E FGG G G G A W+ Y + TI Sbjct: 435 HVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTI 478 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 77/245 (31%), Positives = 123/245 (50%), Gaps = 11/245 (4%) Query: 8 GDDEVQQTIAAARTASLAWRNVPAPVRGELVRLLGEVLREKREALGLLVSLEAGKIRAEG 67 G E ++ I AA A AW+ A R ++R E++ + L L+++ E GK AE Sbjct: 47 GAAETRRAIEAANAAWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEA 106 Query: 68 LGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVITAFNFPVAVWAW 127 GE+ ++ ++ +G TI S P +R++ +GVC IT +NFP A+ Sbjct: 107 RGEIGYAASFIEWFAEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITR 166 Query: 128 NAALALVCGNAVVWKPSEKTPVTALAVQALMRQAIARFGQQRPGAVPAGLCGLLIGGAN- 186 A AL G +V KP+E TP +ALA+ L +A +PAG+ ++ G Sbjct: 167 KAGPALAAGCPMVLKPAEATPFSALALAVLAERA----------GIPAGVFSVVTGTPKG 216 Query: 187 VGAAISASPDVALVSATGSVAMGRKVAVTVARRLGRSLLELGGNNAMIVAPTADLNLALR 246 +G ++++P V +S TGS +G+ + A + + LELGGN IV ADL+ A+ Sbjct: 217 IGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVE 276 Query: 247 AIVFS 251 + S Sbjct: 277 GAIAS 281 Score = 72.4 bits (176), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 50/189 (26%), Positives = 86/189 (45%), Gaps = 5/189 (2%) Query: 332 VGLRRVFIHRSLFDKVCADIERVYAGIRVGDPLAPETLVGPLIDGGAYDQMQQALAQAQK 391 V R+++ ++D A + +VG GPLI+ A +++Q +A A Sbjct: 291 VCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVA 350 Query: 392 EGGNVLGGERVTTFGGEHAYYVRPALVRMPRQSAVMHRETFAPILYAVAYDDVEEAIALN 451 +G VL G + G H+++ L + V ETF P+ + EE +AL Sbjct: 351 KGARVLLGGKRHALG--HSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALA 408 Query: 452 NAVPQGLSSAIFTNDVRESELFMSAQGSDCGLANVNIGTSGAEIGGAFGGEKETGGGRES 511 N GL+S ++ D+ ++ A+G + G+ +N G E+ FGG K++G GRE Sbjct: 409 NDTEFGLASYFYSRDI--GRIWRVAEGLESGMVGINTGLISNEV-APFGGVKQSGLGREG 465 Query: 512 GSDAWKNYM 520 +Y+ Sbjct: 466 SHYGIDDYL 474 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 106 bits (265), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 79/268 (29%), Positives = 124/268 (46%), Gaps = 14/268 (5%) Query: 10 DEVQQTIAAARTASLAWRNVPAPVRGELVRLLGEVLREKREALGLLVSLEAGKIRAEGLG 69 DEV + A A AW N P R ++ +++++ + ++ E GK ++ G Sbjct: 41 DEVDAAVKAGVAAFPAWSNTPPLTRARVLFRFLHLIQQRADDFARIIVREHGKTFSDAQG 100 Query: 70 EVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVITAFNFPVAVWAWNA 129 EV I+I +FA G+ + L G G LGV IT FNFP V W Sbjct: 101 EVARGIEIVEFAAGIPQMLKGEYTDQIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMF 160 Query: 130 ALALVCGNAVVWKPSEKTPVTALAVQALMRQAIARFGQQRPGAVPAGLCGLLIGGANVGA 189 +A+ CGN V KPSE+ P +L + L+++A +P G+ ++ G Sbjct: 161 PIAIACGNCFVLKPSERDPSASLLIADLLKEA----------GLPDGVFNVVQGDKVTVD 210 Query: 190 AISASPDVALVSATGSVAMGRKVAVTVARRLGRSLLELGG-NNAMIVAPTADLNLALRAI 248 A+ P V +S GS + + + + G+ + LGG N M+V P AD+ LA+ A+ Sbjct: 211 ALLDHPQVKAISFVGSTPIAEYIYARGSAK-GKRVQALGGAKNHMVVMPDADMQLAVDAL 269 Query: 249 VFSAVGTAXLGRRRRRPSRHLPAATAGS 276 + +A G+A G R S L +AG Sbjct: 270 MGAAYGSA--GERCMAISVALAVGSAGD 295 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 105 bits (263), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 72/247 (29%), Positives = 116/247 (46%), Gaps = 10/247 (4%) Query: 10 DEVQQTIAAARTASLAWRNVPAPVRGELVRLLGEVLREKREALGLLVSLEAGKIRAEGLG 69 +EV + +A A+ WRN R ++ ++LRE L L++ E GK + G Sbjct: 46 EEVDRAVANAKETFKTWRNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEG 105 Query: 70 EVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVITAFNFPVAVWAWNA 129 EV +++ + A ++ G + G + + PLGV ITAFNFPV + + Sbjct: 106 EVMRGLEVVEHACSITSLQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMF 165 Query: 130 ALALVCGNAVVWKPSEKTPVTALAVQALMRQAIARFGQQRPGAVPAGLCGLLIGGANVGA 189 +A+ CGN V KPSE+ P ++L + L QA +P G+ ++ GG +V Sbjct: 166 PIAVACGNTFVLKPSEQDPTSSLFLVELANQA----------GLPPGVLNVVHGGPDVAN 215 Query: 190 AISASPDVALVSATGSVAMGRKVAVTVARRLGRSLLELGGNNAMIVAPTADLNLALRAIV 249 I PD+ VS GS +G + + R+ +G N IV P A + A+ ++ Sbjct: 216 MICDHPDIKAVSFIGSTHVGTHIYRRASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLL 275 Query: 250 FSAVGTA 256 +A G A Sbjct: 276 GAAFGAA 282 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 101 bits (252), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 120/479 (25%), Positives = 199/479 (41%), Gaps = 88/479 (18%) Query: 11 EVQQTIAAARTASLAWRNVPAPVRGELVRLLGEVLREKREALGLLVSLEAGKIRAEGLGE 70 ++++ I AA A WR V R +++R + RE+ +A+ ++++ GK A+ + E Sbjct: 43 DIEEAIQAAHRAFQQWRQVSPLERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAE 102 Query: 71 VQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVITAFNFPVAVWAWNAA 130 + + D+ R+L+G + + P R + P+GVC I+ +NFP A Sbjct: 103 IINAAEHLDWHAEEGRRLYGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVA 162 Query: 131 LALVCGNAVVWKPSEKTPVTALAVQALMRQAIARFGQQRPGAVPAGLCGLLIG-GANVGA 189 A+ G VV K ++P + + L A VPAG+ ++ G + + Sbjct: 163 AAMAAGCTVVLKGPSESPAAIVLMAQLFHDA----------GVPAGVFNIVSGVSSEISQ 212 Query: 190 AISASPDVALVSATGSVAMGRKVAVTVARRLGRSLLELGGNNAMIVAPTADLNLALRAIV 249 + SP V +S TGSV +G K+A A + R +ELGG+ ++V A++ A R + Sbjct: 213 HLIESPLVRNISFTGSVPVGSKLAAQAALHMKRMSMELGGHAPVLVFDDAEVEPAAR--L 270 Query: 250 FSAVGTAXLGRRRRRPSRHLPAATAGSTFGPEPRGLHRARYSRSLRRRHAHVAQGLGHAT 309 + + T G+ PSR +G++ AR+ + + G GH Sbjct: 271 LARLKTRNAGQVCVSPSRFYVQ-----------KGIY-ARFVTAFAEALLQIKVGPGHEP 318 Query: 310 GPGPSSDLVGLXATGPGPSSDLVGLRRVFIHRSLFDKVCADIERVYAGIRVGDPLAPETL 369 G GP IH F+ V Sbjct: 319 G----------VQMGP------------LIHGRRFEAVQG-------------------- 336 Query: 370 VGPLIDGGAYDQMQQALAQAQKEGGNVLGGERVTTFGGEHAYYVRPALVR-MPRQSAVMH 428 LID ALAQ + V GG R+ G+ ++ P L+ +P + VM Sbjct: 337 ---LID--------DALAQGSE---LVTGGARL----GQRGHFYAPTLITGVPPSARVMQ 378 Query: 429 RETFAPILYAVAYDDVEEAIALNNAVPQGLSSAIFTNDVRESELFMSAQGSDCGLANVN 487 E F PI + EEAIA+ NA+P GL+S +FT + + ++ G+ N+N Sbjct: 379 EEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTAHRV--SRDLQAGMVNIN 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,546,987 Number of extensions: 61582 Number of successful extensions: 328 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 29 Length of query: 544 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 454 Effective length of database: 1,193,720 Effective search space: 541948880 Effective search space used: 541948880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)