BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0021.p (480 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding prote... 263 3e-71 gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-me... 93 4e-20 gi|300311907|ref|YP_003775999.1| ankyrin repeat-containing signa... 65 2e-11 >gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 384 Score = 263 bits (671), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 134/336 (39%), Positives = 192/336 (57%), Gaps = 3/336 (0%) Query: 141 GADINLTPNAVRALDGLGIADAVRRTAARPTHRISRTWDSGEETSRLEMGDTAEKKYGAP 200 GA I++ PN ++ L +GI D + R +RP SR W +G+ +++ +GD A K YGA Sbjct: 43 GAGIHVGPNVMKVLRRIGIEDEMNRQGSRPDFWYSRHWQTGDVLAQIPLGDYAVKHYGAS 102 Query: 201 QLTIHRADLLAALAEVFPAGRVQFAKRAEAIVEDAQGITLSFTDGTTDKVDVLIGGDGIH 260 LT+HR D L + P+ R+ F K + + + LSF DGTT++ D++IG DG++ Sbjct: 103 YLTVHRGDFHELLIKALPSERLSFGKCLTKVEDRGDVVILSFADGTTEEADIVIGADGVN 162 Query: 261 SAVRSAMFGKESPRFTGVVAFRAVIPADSV-AQVPNLQAFTKWWGPDPESQIVTFPLNCG 319 S +R + G E P++ G +A RAV P + A + A KWW D +V F + Sbjct: 163 SRIREELLGVEPPKYAGYLAHRAVFPTPELKAGMLPFDACVKWWS-DDRHMMVYFVTSKA 221 Query: 320 KDIFIFATTAQETWHLESWTTPGSVQELRDSYAGFHPDATALLDACDEVLKTALYERDPL 379 +++ E W L P S +E+R+++ G+HP AL+DA EV K +L ERDPL Sbjct: 222 NELYYVTGVPVEKWDLNDRWLPSSKEEMRETFHGWHPTVQALVDATVEVTKWSLLERDPL 281 Query: 380 PHWSKGHMTLLGDASHPMMPFMAQGAGMAIEDAVVLARCLEKVASVDGIDEALKTYQELR 439 P WS+G + LLGDA HPM P MAQGA MAIED +LARC ++V V +A Y+ R Sbjct: 282 PLWSRGRLVLLGDACHPMKPHMAQGAAMAIEDGAMLARCFKEVG-VSNYADAFALYEANR 340 Query: 440 LERTSKIQIGSRGNNWLREGGNADWVYGYDAWSIPL 475 R SK+Q S N WLR + W +GYD ++ PL Sbjct: 341 AARASKVQRISHDNTWLRTNEDPSWCFGYDVFTEPL 376 >gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 93.2 bits (230), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 92/350 (26%), Positives = 158/350 (45%), Gaps = 36/350 (10%) Query: 130 RVEIVEL---LLAHGADINLTPNAVRALDGLGIADAVRRTAARPTHRISRTWDSGEETSR 186 +V++VEL +GA I+L P +RAL LG+ +A R A + G + + Sbjct: 30 QVDLVELDAQWRNYGAGISLGPATLRALKQLGVLEAFLREGA-AGDGVRLCLPHGPQVAE 88 Query: 187 LEMGDTAEKKYGAPQLTIHRADLLAALAEVFP------AGRVQFAKRAEAIVEDAQGITL 240 L + +P + A L LA + V+ A+ E + + Sbjct: 89 LPT-----PRLASPDVPGGGAILRPVLARILADATRAAGADVRLGCTFSAVREVGHKVEV 143 Query: 241 SFTDGTTDKVDVLIGGDGIHSAVRSAMFGK-ESPRFTGVVAFRAVIPADSVAQVPNLQAF 299 FTDG T + D++IG DG++S +R +F PR++G +RAV+P + Sbjct: 144 DFTDGQTRRYDLVIGADGLYSKLRMHLFPHAPKPRYSGQAVWRAVLPRPQ-----EIVTC 198 Query: 300 TKWWGPDPESQIVTFPLNCGKDIFIFATTAQET-WHLESWTTPGSVQELRDSYAGFHPDA 358 T W GP + + + ++++F T + H++ T ++ L + ++ P Sbjct: 199 TMWMGPRIKPGVNPVSKD---EMYLFVTEPRPVNEHVDPTTFVSHLRGLLEEFSA--PVL 253 Query: 359 TALLDACDEVLKTALYERD----PLPHWSKGHMTLLGDASHPMMPFMAQGAGMAIEDAVV 414 + + D+ + + P P W +G + L+GDA H P +A GA + IEDA+V Sbjct: 254 KTIREQLDDNARIIFRPLEGLLLPRP-WYQGRVVLIGDAVHATTPHLASGACIGIEDALV 312 Query: 415 LARCLEKVASVDGIDEALKTYQELRLERTSK-IQIGSRGNNWLREGGNAD 463 LA LE+ +V +AL ++E R ER ++ +R EGG+ D Sbjct: 313 LADELERHGTV---PQALAAFEERRWERCRMVVENSARLGEIEVEGGDKD 359 >gi|300311907|ref|YP_003775999.1| ankyrin repeat-containing signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 64.7 bits (156), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 44/116 (37%), Positives = 66/116 (56%), Gaps = 9/116 (7%) Query: 51 KGDSLLMLAAYHGHAAAVRVMLQHKADAGLRNDNGQTPIAGAAFKGHVAVVQALLDGGAD 110 GD+ LMLA+++G+A V+++L + + N G T + AA G +V+ LLD A Sbjct: 100 NGDTPLMLASFYGNAPVVKLLLAREVEV---NRPGWTALHYAAINGSSEIVKLLLDASAY 156 Query: 111 IEGASPDGK-TALMIAAMFNRVEIVELLLAHGADINLTPNAVRALDGLGIADAVRR 165 ++ SPD K T +M+AAM RV VE+L +GAD++L DGL D +R Sbjct: 157 VDCESPDDKMTPVMLAAMRGRVAAVEVLRDNGADLSLKNK-----DGLTAMDLAKR 207 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,675,768 Number of extensions: 74244 Number of successful extensions: 258 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 3 Length of query: 480 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 391 Effective length of database: 1,198,455 Effective search space: 468595905 Effective search space used: 468595905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)