BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0018.p (305 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313279|ref|YP_003777371.1| vanillate O-demethylase oxygena... 71 9e-14 gi|300309801|ref|YP_003773893.1| phenylpropionate dioxygenase [H... 63 3e-11 >gi|300313279|ref|YP_003777371.1| vanillate O-demethylase oxygenase subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 71.2 bits (173), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 41/126 (32%), Positives = 65/126 (51%), Gaps = 3/126 (2%) Query: 22 GWPLSMGIAMGDRIACGYHGVQVRFDGVVMSVPGSPGCKLEGAKATRSYHVTEACGAIFL 81 G PLS+G ++ CGYHG+++ DG +S+PG ++ G RSY V E G I++ Sbjct: 51 GAPLSLGFVREGQLVCGYHGLEMGCDGKAISMPGQ---RVRGFPCIRSYPVEERYGFIWV 107 Query: 82 FNAGSDIAEPPPLRLPEELTSPEYSNFLCYTEWKGDYRYVLDNVMDPMHGTFLHKQSHSM 141 + + A+P + E +PE++ DYR ++DN+MD H T++H S Sbjct: 108 WPGDKEQADPAQIHHLEWADNPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQ 167 Query: 142 AEGDNT 147 E D Sbjct: 168 KEIDEA 173 >gi|300309801|ref|YP_003773893.1| phenylpropionate dioxygenase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 63.2 bits (152), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 74/295 (25%), Positives = 115/295 (38%), Gaps = 43/295 (14%) Query: 25 LSMGIAMGDRIACGYHGVQVRFDGVVMSVPGSPGCKLEGAKATRSYHVTEACGAIFLFNA 84 LS G I CGYHG +G + VP + + +SY E G ++ Sbjct: 60 LSKGFVENGNIVCGYHGWTFNGEGACVRVPQNADGSVPAGACVKSYFCKERYGYVW---- 115 Query: 85 GSDIAEPPPLR-LPE--ELTSPEYSN-FLCYTEWKGDYRYVLDNVMDPMHGTFLHKQSHS 140 +A PLR +PE E +P Y F Y W+ +++N D H +++H+ + Sbjct: 116 ---VALEEPLRDIPEFPEEGAPGYRRIFQFYERWETSALRMMENSFDNSHFSYVHRATFG 172 Query: 141 MAEGDNTASFGIRETDIGFIFEKEGQRDV-NFDWT-----EWADTDLHWM---------- 184 + + + I ETD G FE E + + N + ADT + M Sbjct: 173 LYDQPKPEKYEIAETDYG--FEAETRVPIKNVPASHRVTGSTADTTVRHMFNRWYLPFVR 230 Query: 185 RLAIPYPKTGGPGGSFYIVGSYTPIVDNIAAVFHWRCRKLTGWQKDTWRFLYRNRLEARH 244 R YP++ G I TPI D + W ++ DT L A Sbjct: 231 RFGCTYPES---GRHHIIYNCATPIDDGSIMLSQWL------YRNDTEEECSEAELIAWD 281 Query: 245 WNVLEQDRAVLEVMEPDA-----NQREMLYQHDMGIVRLRRHMRNLAKEQLAKEA 294 +L +DR +LE +PDA ++E D + +RR + L +E E Sbjct: 282 APILVEDREILEATDPDACIDVRRRQEFHMASDRPGLIMRRMLLQLLEEHGESEV 336 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 924,857 Number of extensions: 37700 Number of successful extensions: 85 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 82 Number of HSP's successfully gapped: 2 Length of query: 305 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 220 Effective length of database: 1,217,395 Effective search space: 267826900 Effective search space used: 267826900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)