BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0016.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 250 1e-67 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 207 8e-55 gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 192 2e-50 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 62 4e-11 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 250 bits (638), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 147/320 (45%), Positives = 190/320 (59%), Gaps = 10/320 (3%) Query: 4 SLLSVRVQAIRFEAQGIVSVELVSPEGAELPAFEAGSHVDLHLANGIVRSYSLLNAPAER 63 + L V+V A R EA I+++ELV+ +GA LPAF AG+H+D+HL G+VR YSL NAP E+ Sbjct: 2 TTLQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQ 61 Query: 64 HRYVVGVLNDRNSRGGSRYVHEQLRVGATLRISAPRNNFPVDESATHSVLIAGGIGITPI 123 HRY +GVL D NSRGGS VHE L++G L ISAPRN F + A HS+L+AGGIG+TPI Sbjct: 62 HRYQIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFAL-VPAQHSLLLAGGIGVTPI 120 Query: 124 SCMLNYLRGKGKSAELLYCARSRAEAAFASELLQQTG----VSSHF-DAEKGAPPDLRAF 178 CM L G S E+ YCARS A AF E + +G V H+ D E DL A Sbjct: 121 LCMAEALAAAGASFEMHYCARSPARQAF-RERIAASGFAARVVHHYDDGEAAQKLDLDAL 179 Query: 179 LASKPKDAHFYCCGPTPMLNAFEAICAELGLP--NVHIERFAAAENVEAVQGDEYVAELA 236 LA H Y CGP + G P VH E F AA V+A + +LA Sbjct: 180 LAQADPATHLYVCGPAGFIAHVVDTARARGWPEAQVHFEYFGAAP-VKADGNGAFEVKLA 238 Query: 237 RSKKSVTVPAGKSLLDALLDAGLDVEHSCREGVCGACETKVLEGEPEHRDGVLSKAEKAS 296 S + T+P ++++ L + G+DV SC +G+CG C T+VLEGEP+HRD L+ E+A+ Sbjct: 239 SSGQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAA 298 Query: 297 NKMMMVCVSGCKGRRLVLDL 316 N C S + LVLDL Sbjct: 299 NDQFTPCCSRARSALLVLDL 318 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 207 bits (527), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 136/329 (41%), Positives = 193/329 (58%), Gaps = 15/329 (4%) Query: 2 SSSLLSVRVQAIRFEAQGIVSVELVSPEGAELPAFEAGSHVDLHLANGIVRSYSLLNAPA 61 +++ L +RV+A+R AQ I +ELVS +GA LP + G+H+ L L +G+VR+YSL N P Sbjct: 12 AATTLMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQ 71 Query: 62 ERHRYVVGVLNDRNSRGGSRYVHEQLRVGATLRISAPRNNFPVDESATHSVLIAGGIGIT 121 +R Y++ V + +SRGGS ++HEQ++ G T+ S P N FP+ +L+A GIGIT Sbjct: 72 QRDCYLIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGIT 131 Query: 122 PISCMLNYLRGKGKSAELLYCARSRAEAAFASELLQQT-GVSSHFDAEKGAPPD--LRAF 178 P+ M L +G+ L Y ARS A AAF EL + G+S H + GA + + A Sbjct: 132 PLHAMAAELATQGRRHRLHYFARSLAHAAFFHELAAGSQGMSLHLGLD-GAETEHAIAAA 190 Query: 179 LASKPKDAHFYCCGPTPMLNAF--EAICAELGLPNVHIERF-------AAAENVEAVQGD 229 LA+ P+ + Y CGP+P ++A A A ++H ERF A A+ VQ Sbjct: 191 LAAAPRSSPLYVCGPSPFIDAARRHAQLAGWREQDIHFERFAAPAMPAAEADPARPVQAP 250 Query: 230 EYVAELA--RSKKSVTVPAGKSLLDALLDAGLDVEHSCREGVCGACETKVLEGEPEHRDG 287 + EL RS V G+S++ A G+ + SC EG CG+CE+ VLEG+P HRD Sbjct: 251 DSTFELVLQRSGLRCQVLPGQSIVAAAAQVGVVIGTSCGEGFCGSCESTVLEGQPWHRDS 310 Query: 288 VLSKAEKASNKMMMVCVSGCKGRRLVLDL 316 VLS AE+AS + ++ CVS C G RLVLDL Sbjct: 311 VLSAAERASGRRILPCVSRCAGTRLVLDL 339 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 192 bits (489), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 119/319 (37%), Positives = 167/319 (52%), Gaps = 9/319 (2%) Query: 6 LSVRVQAIRFEAQGIVSVELVSPEGAELPAFEAGSHVDLHLANGIVRSYSLLNAPAERHR 65 + + V + E I S LV +GA LPA+E G+H+D+ GI+R YSL +R Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 66 YVVGVLNDRNSRGGSRYVHEQLRVGATLRISAPRNNFPVDESATHSVLIAGGIGITPISC 125 Y + V + SRGGS +H+ ++ G L + PRN F +D SA +L GIG+TP+ Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 126 MLNYLRGKGKSAELLYCARSRAEAAFASELLQQTGVSSHFDAEKGAPPD-----LRAFLA 180 M L + K L Y R+R AAFA +LL+ ++H P L L Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFA-DLLENAPFAAHVRFHYAVMPAQMEHYLTVCLE 179 Query: 181 SKPKDAHFYCCGPTPMLNA-FEAICAELGLPNVHIERF--AAAENVEAVQGDEYVAELAR 237 + K+AH Y CGP P ++A + A +H+E F A A A + +LA Sbjct: 180 AGSKEAHVYTCGPAPFMDAVVKCASATRSEEAIHLEHFQAAPAAPAAAGGDGAFEVKLAS 239 Query: 238 SKKSVTVPAGKSLLDALLDAGLDVEHSCREGVCGACETKVLEGEPEHRDGVLSKAEKASN 297 S K V +P G ++D L G+ ++ SCREG+CG C +LEGEP+HRD LSK EKA+N Sbjct: 240 SGKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAN 299 Query: 298 KMMMVCVSGCKGRRLVLDL 316 + CVS + LVLDL Sbjct: 300 DQICTCVSRARSGMLVLDL 318 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 62.4 bits (150), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 60/220 (27%), Positives = 92/220 (41%), Gaps = 29/220 (13%) Query: 16 EAQGIVSVELVSPEGAELPAFEAGSHVDLHL---ANGIVRSYSL---LNAPAERHRYVVG 69 E++ I S V +G EL F G ++ L + R YSL L + +Y + Sbjct: 166 ESEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQAGQPPQYRIS 225 Query: 70 VLNDRNSRGG--SRYVHEQLRVGATLRISAPRNNFPVDESATHSVLIAGGIGITPISCML 127 V + GG SR +H+Q+ VG+ + + P F + S LI+GG+GITP+ ML Sbjct: 226 V---KREPGGKVSRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGITPMMSML 282 Query: 128 NYLRGKGKSAELLYCARSRAEAAFASEL----LQQTGVSSHFDAEKGAPPD--------- 174 G+ ++ AR AF +L + + F E+ D Sbjct: 283 AAALPSGRPIHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGDAAPHATGLI 342 Query: 175 ----LRAFLASKPKDAHFYCCGPTPMLNAFEAICAELGLP 210 L+ +L P D Y GP + A + AELG+P Sbjct: 343 ERSLLQQWLPGNP-DLDAYFVGPKSFMKAIKRQLAELGVP 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 890,892 Number of extensions: 34941 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 4 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)