BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0015.p (346 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313395|ref|YP_003777487.1| porin transmembrane protein [He... 168 5e-43 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 145 5e-36 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 140 1e-34 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 139 3e-34 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 130 2e-31 >gi|300313395|ref|YP_003777487.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 168 bits (426), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 126/336 (37%), Positives = 171/336 (50%), Gaps = 35/336 (10%) Query: 18 FAQSNVTIYGSLDAGVAYVSNLGGGSVARVDQGTMQPDRIGFRGTEDLGGNLKANFQLET 77 +AQS+VT+YG++D G+AY SN GG S ++ G + ++G GTEDLGG A F+LE Sbjct: 32 WAQSSVTLYGTVDLGLAYSSNQGGASNTYMNSGALAASKLGLTGTEDLGGGNMALFRLEN 91 Query: 78 GFYTDSGAQPSAGKLFNRYSTVGLSG-DFGAVTMGHMPDIVFDYVGKLSNGYQLTNWYLF 136 GF D+GAQ +AG LFNR S VGLS D+G VT+G F YVG L LT Sbjct: 92 GFNADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTNVLTGATGA 151 Query: 137 HPGNLDSLANTYQFDNAVRYTSPTFAGLQLSGMYGFGEVAGDNSNGRNISAGATYTNGPL 196 HPG++D+L T + +N++ YT PT GLQ YG GE AG +NG ++SA Y Sbjct: 152 HPGDIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRYDYQAF 211 Query: 197 RAALAYSKLNNRAAG-----YG--GAFLQSLGLGTAATVFNSLSTTAAGVGYTIGPVRLN 249 + Y++L N A G +G G+F + + +S A YT G + L Sbjct: 212 SWSAGYTRLKNLANGNTAGSFGNVGSFANNSPVNAGYASADSTQLLATAARYTRGDLMLG 271 Query: 250 ADYTQVK-----------------ISL--PTGVTPKQKNFDLGAAWRYAAADTLNFGYGL 290 +Y+ V+ I L V+P L A + Y A N G+ Sbjct: 272 LNYSNVQYKAGALSAFSQTAVFNTIGLIGSYNVSPA---LVLAAGYSYTAEKARN---GI 325 Query: 291 SKLESARWNTFSLSHVHAFSKRTEFYVQAAYQRAGG 326 S A++N SL V++ SKRT Y AYQRA G Sbjct: 326 S--SPAKYNQLSLEEVYSLSKRTALYAIQAYQRASG 359 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 145 bits (366), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 104/295 (35%), Positives = 153/295 (51%), Gaps = 28/295 (9%) Query: 1 MKKVLFAVAATG-FAASAFAQSN-VTIYGSLDAGVAYVSNLGG-----GSVARVDQGTMQ 53 MKK L A+A G FA +A AQS+ +TIYG++DA + Y + + GS+ V+ G ++ Sbjct: 1 MKKSLLALAVLGAFAGAAHAQSSSITIYGAIDASLTYTNKIATDGKNTGSLTAVESGLVK 60 Query: 54 PDRIGFRGTEDLGGNLKANFQLETGFYTDSGAQPSAGKLFNRYSTVGLSGDFGAVTMGHM 113 R+G +G+EDLGG LKA F LE GF D+G ++ R +TVGLSG+FG V +G Sbjct: 61 GSRLGIKGSEDLGGGLKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGLSGNFGTVLVGRQ 120 Query: 114 PDIVFDYVGKLSNGYQLTNWYLFHPGNLDSLANTYQFDNAVRYTSPTFAGLQLSGMYGFG 173 D + D +G +++ H LD + + +N++RY SP F GL SG+YGFG Sbjct: 121 NDPLND-IGGIASAGGFGVPGTVHSKGLDR-TDGQRLNNSIRYNSPNFNGLSFSGIYGFG 178 Query: 174 EVAGDNSNGRNISAGATYTNGPLRAALAY------------SKLNNRAAGYGGAFLQSLG 221 E G GR+I G+ Y NGPL L Y S +N +AA G G Sbjct: 179 EQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGSSDVNAKAACNGAGEKDHAG 238 Query: 222 LGTAATVFNSLSTTAAGVGYTIGPVRLNADYTQVKISLPTGVTPKQKNFDLGAAW 276 T + + AA Y GP ++ ++ V++ L T +K+ + GA + Sbjct: 239 ----DTCLKTWTLAAA---YQFGPAKVYGAFSHVRLPLAKLHTKVRKDNNFGAMY 286 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 140 bits (353), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 114/322 (35%), Positives = 155/322 (48%), Gaps = 28/322 (8%) Query: 16 SAFAQSNVTIYGSLDAGVAYVSNLG---GGSVARVDQGTMQPDRIGFRGTEDLGGNLKAN 72 S AQS+VTIYG +D GV Y++N G GSV R+ G RIGFRGTEDLGG LKA Sbjct: 18 SVQAQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTSGATNTSRIGFRGTEDLGGGLKAL 77 Query: 73 FQLETGFYTDSGA-QPSAGKLFNRYSTVGLSGDFGAVTMGHMPDIVFDYVGKLSN-GYQL 130 FQLE GF D+G+ A +LFNR STVGL G FG V G +D++G GY Sbjct: 78 FQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGYSA 137 Query: 131 TNWYLFHPGNLDSLANTYQ--FDNAVRYTSPTFAGLQLSGMYGFGEVAGDNSNGRNISAG 188 + G + + N V+Y F+G+++ MYGFGEVAG+ NG G Sbjct: 138 NYSWATTAGATGNRKDGMPTGVSNLVKYQGE-FSGVKIGAMYGFGEVAGETRNGAKYGVG 196 Query: 189 ATYTNGPLRAALAYSKLNNRAAGYGGAFLQSLGLGTAATVFNSLSTTAAGVGYTIGPV-R 247 + GP Y + N+ A T ++ ++ GY + Sbjct: 197 LGWGTGPFSVVATYDRNNSATA--------------TNTPYDRATSAHLAAGYQPSEAWK 242 Query: 248 LNADYTQVKISLPTGVTPKQKNFDL-GAAWRYAAADTLNFGYGLSKLES----ARWNTFS 302 N Y K SL TG + +F GAA+++ A TL +++ A +S Sbjct: 243 FNLGYRYYKKSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDADPIMYS 302 Query: 303 LSHVHAFSKRTEFYVQAAYQRA 324 L +A SKRT+ Y+ AY +A Sbjct: 303 LRLKYAMSKRTDLYLSTAYAKA 324 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 139 bits (350), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 116/353 (32%), Positives = 158/353 (44%), Gaps = 39/353 (11%) Query: 1 MKKVLFAVAA-TGFAASAFAQSNVTIYGSLDAGVAYVSNL-----GGGSVARVDQGTMQP 54 MKK L A G A +A AQS+V+IYG +D G+ Y S+ GG + R+D G Q Sbjct: 1 MKKTLLVAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQG 60 Query: 55 DRIGFRGTEDLGGNLKANFQLETGFYTDSGAQPSAGKLFNRYSTVGL-SGDFGAVTMGHM 113 RIGF+GTEDLGG + A F LETGF D G+ G LF R S VGL S +G++++G Sbjct: 61 SRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQ 120 Query: 114 PDIVFDYVGKLSNGYQLT----NWYLFHPGNLDSLANTYQF-----DNAVRYTSPTFAGL 164 D + + G+ + G W L + N + + +N+++Y S F G Sbjct: 121 YDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGF 180 Query: 165 QLSGMYGFGEVAGDNSNGRNISAGATYTNGPLRAALAYSKLNNRAAGYGGAFLQSLGLGT 224 M G GE AG+N+ GR +SA Y NGP ALAY+ + + + Sbjct: 181 SFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSF------------ 228 Query: 225 AATVFNSLSTTAAGVGYTIGPVRLNADYTQVKISLPTGVTPKQKNFDLGAAWRYAAADTL 284 S G Y IG TQVK T + K ++LG + L Sbjct: 229 ------STRIYGGGARYQIGAFTPFGVVTQVK---NTNTSAKATTYELGTVYALTPLWDL 279 Query: 285 NFGYGLSKLESARWNTFSLSHV--HAFSKRTEFYVQAAYQRAGGSARFAVMNG 335 Y K N +L + SKRT+ YV A Y R G + V G Sbjct: 280 AAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYVGAVYDRDKGYNAYPVFGG 332 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 130 bits (326), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 117/363 (32%), Positives = 168/363 (46%), Gaps = 41/363 (11%) Query: 1 MKKVLFAVAA-TGFAASAFAQSNVTIYGSLDAGVAYVSNL-----GGGSVARVDQGTMQP 54 MKK L A+A FA +A AQS+VTIYG +D GV Y S + G GS ++ G +Q Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQG 60 Query: 55 DRIGFRGTEDLGGNLKANFQLETGFYTDSGA---QPSAGKLFNRYSTVGLSGDFGAVTMG 111 RIGF+GTEDLGG L A F LE GF D+GA ++ LF R S VGL+G+FG+V +G Sbjct: 61 SRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLLG 120 Query: 112 HMPDIVFDYVGKLSNGYQLTNWYLFHPGNLDSLANTYQFDNAVRYTSPTFAGLQLSGMYG 171 D D + ++ W NLD L T + +N++ YT+ G + MYG Sbjct: 121 RQTDFA-DTISSYTSVQDFGGWTGAAGHNLDRLEGT-RTNNSISYTTANLNGFTGNLMYG 178 Query: 172 FGEVAGDNSNGRNISAGATYTNGPLRAALAYSKLNNRAAGYGGAFLQSLGLGTAATVFNS 231 FGE AG S G+ G Y NGPL + Y + + + L + G N+ Sbjct: 179 FGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQFTNGTNN 238 Query: 232 LSTTA----AGVGYTIGPVRLNADYTQVKISLPT--------------------GVTPKQ 267 ST Y GP R+ ++++VK L T + K Sbjct: 239 GSTAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKA 298 Query: 268 KNFDLGAAWRYAAADTLNFGYGLSKLE------SARWNTFSLSHVHAFSKRTEFYVQAAY 321 +++G A+ + + L ++ + A+ SL + SKRT+ Y AY Sbjct: 299 DIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDLYAFLAY 358 Query: 322 QRA 324 +A Sbjct: 359 SKA 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,122,741 Number of extensions: 51122 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 14 Length of query: 346 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 260 Effective length of database: 1,212,660 Effective search space: 315291600 Effective search space used: 315291600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)