BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0014.p (442 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 296 2e-81 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 291 7e-80 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 286 2e-78 gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis pro... 285 4e-78 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 281 6e-77 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 296 bits (758), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 189/407 (46%), Positives = 261/407 (64%), Gaps = 4/407 (0%) Query: 1 LKLGTRLGLAFGLVVLLLIGITGLGIQSLGALHDGTQLIVKNRYPQVVVANDIVLQISDN 60 +K+ RL + F +++ ++ ITG+G++SL ++ + LIV++RY ++ +A +I ++ Sbjct: 6 IKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRDNVNGA 65 Query: 61 AIAMRNILLIDDHDTLLKEIQRIEDGEKSINANLVTLRQMLSSDMGRKSYGEISALRATY 120 A +RN LL + + + + R L + ++LS+ GR+ + I R+ Y Sbjct: 66 ARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQEARSAY 125 Query: 121 EKGQKEFLKLAATGATMDASQLLLTSLRNDQQNYINRVKGFIKGGSIVMAKSSVEASDLY 180 + + +L +A +LL T + Q Y + F+ +M +S E D Sbjct: 126 NTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTEGRDTT 185 Query: 181 RNKTVIMLSMAAIAVLLACGFAYW-ITRSITRPVNHAVQVAQSVAAGDLSSHVEVRSQDE 239 ++ +ML ++A+A+ L C A W +TRSITRP+N AV VA +VAAGDL+ + S+DE Sbjct: 186 QSAIAMMLGLSAVAIAL-CVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGATSKDE 244 Query: 240 IGQLLMALRDMNGSLTNIVSEVRTGADTIATASSEIATGNLDLSSRTEQQAGSLEETAAT 299 G LL AL+ MN SL IVSEVR G+DTI TAS+EIATGNLDLSSRTE+QAG+LEETA+ Sbjct: 245 TGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEETASA 304 Query: 300 MEQLTSTVKQNADNARQANQLAATASDVAAHSGSVVAQVVTTMGSISESSRKIVDIISVI 359 ME+LTSTVKQNADNARQAN LAATAS+VA GSVV QVV TMG I+++SRKIVDIISVI Sbjct: 305 MEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIVDIISVI 364 Query: 360 DGIAFQTNILALNAAVEA--HAPASRAAALRLSPAKCAAWRNARPAR 404 DGIAFQTNILALNAAVEA R A+ S + A R+A A+ Sbjct: 365 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAK 411 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 291 bits (745), Expect = 7e-80, Method: Compositional matrix adjust. Identities = 187/410 (45%), Positives = 257/410 (62%), Gaps = 2/410 (0%) Query: 1 LKLGTRLGLAFGLVVLLLIGITGLGIQSLGALHDGTQLIVKNRYPQVVVANDIVLQISDN 60 +K+ RLG F +++++ I G+G+++L + D T IV +RY +V + I +++ Sbjct: 7 MKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQITDKVNTG 66 Query: 61 AIAMRNILLIDDHDTLLKEIQRIEDGEKSINANLVTLRQMLSSDMGRKSYGEISALRATY 120 A ++RN ++ + + K + R++ ++ +L + + ++ G + + RA Y Sbjct: 67 ARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTNTPRGSELLAALKQARADY 126 Query: 121 EKGQKEFLKLAATGATMDASQLLLTSLRNDQQNYINRVKGFIKGGSIVMAKSSVEASDLY 180 K + + + L +A+ L Q Y VK + S +M KS+ AS Sbjct: 127 SKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDKSAERASAAS 186 Query: 181 RNKTVIMLSMAAIAVLLACGFAYWITRSITRPVNHAVQVAQSVAAGDLSSHVEVRSQDEI 240 + IML ++ +AVLL+ A ITRSITRP+N AVQVA +VAAGDL+ +E S+DE Sbjct: 187 SSAINIMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQIEATSKDET 246 Query: 241 GQLLMALRDMNGSLTNIVSEVRTGADTIATASSEIATGNLDLSSRTEQQAGSLEETAATM 300 G LL AL+ MN +L IV+EVR G DTI TAS EIATGNLDLSSRTEQQAG+LEETA+ M Sbjct: 247 GALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALEETASAM 306 Query: 301 EQLTSTVKQNADNARQANQLAATASDVAAHSGSVVAQVVTTMGSISESSRKIVDIISVID 360 E+LTSTV+QNADNARQAN LA TAS VA GSVV +VV TMG I+++SRKIVDIISVID Sbjct: 307 EELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVDIISVID 366 Query: 361 GIAFQTNILALNAAVEA--HAPASRAAALRLSPAKCAAWRNARPARRKRS 408 GIAFQTNILALNAAVEA R A+ S + A R+A A+ +S Sbjct: 367 GIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKS 416 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 286 bits (733), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 171/407 (42%), Positives = 255/407 (62%), Gaps = 2/407 (0%) Query: 1 LKLGTRLGLAFGLVVLLLIGITGLGIQSLGALHDGTQLIVKNRYPQVVVANDIVLQISDN 60 L + TRL +AF +++L++ GI LG+ L ++ L+V +RY ++ + N I ++ D Sbjct: 6 LSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGKVDDV 65 Query: 61 AIAMRNILLIDDHDTLLKEIQRIEDGEKSINANLVTLRQMLSSDMGRKSYGEISALRATY 120 AI++RN LL+ D + E + IE L +++++ + +++ R +Y Sbjct: 66 AISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTETRKSY 125 Query: 121 EKGQKEFLKLAATGATMDASQLLLTSLRNDQQNYINRVKGFIKGGSIVMAKSSVEASDLY 180 +K+ KL ++G A +L+L+ + Q+ Y +++ +M +S +A Y Sbjct: 126 AALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQSVDQAEAAY 185 Query: 181 RNKTVIMLSMAAIAVLLACGFAYWITRSITRPVNHAVQVAQSVAAGDLSSHVEVRSQDEI 240 +IM+ +A A A+ I+RSITRP++ AV+VA++VAAGDL++ + S DE Sbjct: 186 ETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVIAAHSNDET 245 Query: 241 GQLLMALRDMNGSLTNIVSEVRTGADTIATASSEIATGNLDLSSRTEQQAGSLEETAATM 300 G+LL AL MN L IV EVR G D++ TAS++IATGNLDLSSRTE+QA +LEETA+++ Sbjct: 246 GRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASALEETASSL 305 Query: 301 EQLTSTVKQNADNARQANQLAATASDVAAHSGSVVAQVVTTMGSISESSRKIVDIISVID 360 E+LTS+VKQNAD+ R++++LAA AS VA G V QVV TMG+I++SS KIVDIISVID Sbjct: 306 EELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKIVDIISVID 365 Query: 361 GIAFQTNILALNAAVEA--HAPASRAAALRLSPAKCAAWRNARPARR 405 GIAFQTNILALNAAVEA R A+ S + A R+A A+ Sbjct: 366 GIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKE 412 >gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 534 Score = 285 bits (730), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 175/373 (46%), Positives = 241/373 (64%), Gaps = 2/373 (0%) Query: 6 RLGLAFGLVVLLLIGITGLGIQSLGALHDGTQLIVKNRYPQVVVANDIVLQISDNAIAMR 65 RL L FGL+++L++ + GI LG L+ + ++ ++YP+ VVA DIV Q++ A ++R Sbjct: 11 RLWLGFGLLMVLMLAMAASGIARLGQLNAQMKDVIHDKYPKTVVAGDIVDQLNLIARSVR 70 Query: 66 NILLIDDHDTLLKEIQRIEDGEKSINANLVTLRQMLSSDMGRKSYGE-ISALRATYEKGQ 124 NILL+ + E +RI +++I +L L ++L+ R + ++A A +EK Sbjct: 71 NILLLKREPQIKSEYERISGADRAIREDLAQLDKLLTDGDSRAALTTLVTAQNAYFEKRD 130 Query: 125 KEFLKLAATGATMDASQLLLTSLRNDQQNYINRVKGFIKGGSIVMAKSSVEASDLYRNKT 184 K L + G A +L+ +R Q +N I+ +M + E Y Sbjct: 131 K-VLAMVQQGGKEGAIDMLIDEVRPVQSATMNAADALIRQQHEMMKTAGDEVESNYAQAR 189 Query: 185 VIMLSMAAIAVLLACGFAYWITRSITRPVNHAVQVAQSVAAGDLSSHVEVRSQDEIGQLL 244 ++L +AA + A A+ ITRSIT P++ AV+VA++VAAGDL+S ++ +DE LL Sbjct: 190 TLLLLLAAAGLAAAAVIAWMITRSITAPLDRAVRVAETVAAGDLTSAIDAHGRDETSMLL 249 Query: 245 MALRDMNGSLTNIVSEVRTGADTIATASSEIATGNLDLSSRTEQQAGSLEETAATMEQLT 304 AL+ MN L +IVS+VR DTIATASSEIA GNLDLSSRTE+QA SLEETA++MEQLT Sbjct: 250 RALKKMNDRLLDIVSQVRVSTDTIATASSEIARGNLDLSSRTEEQAASLEETASSMEQLT 309 Query: 305 STVKQNADNARQANQLAATASDVAAHSGSVVAQVVTTMGSISESSRKIVDIISVIDGIAF 364 +TVKQNA +A QA+QLA +ASD+A G VV VV TMG+I SS+KIVDII VIDGIAF Sbjct: 310 ATVKQNASHAGQASQLAGSASDIARQGGEVVGHVVETMGAIDASSKKIVDIIGVIDGIAF 369 Query: 365 QTNILALNAAVEA 377 QTNILALNAAVEA Sbjct: 370 QTNILALNAAVEA 382 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 281 bits (720), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 175/413 (42%), Positives = 248/413 (60%), Gaps = 16/413 (3%) Query: 1 LKLGTRLGLAFGLVVLLLIGITGLGIQSLGALHDGTQLIVKNRYPQVVVANDIVLQISDN 60 LK+G RL + F +++L+ + LG+ LG++ D T + + + A I +N Sbjct: 4 LKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKEN 63 Query: 61 AIAMRNILLIDD---HDTLLKEIQ----RIEDGEKSINANLVTLRQMLSSDMGRKSYGEI 113 + ++ DD K+I RI +K + A + + ++ + E+ Sbjct: 64 GVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEE-------KRLFDEV 116 Query: 114 SALRATYEKGQKEFLKLAATGATMDASQLLLTSLRNDQQNYINRVKGFIKGGSIVMAKSS 173 LRA Y ++ + G A ++ T+L +Y + V + V+ +++ Sbjct: 117 GKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTA 176 Query: 174 VEASDLYRNKTVIMLSMAAIAVLLACGFAYWITRSITRPVNHAVQVAQSVAAGDLSSHVE 233 + YR+ ++L++ AI ++L A+ +TRSITRP+N AV +A++VAAGDL+S ++ Sbjct: 177 AAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRID 236 Query: 234 VRSQDEIGQLLMALRDMNGSLTNIVSEVRTGADTIATASSEIATGNLDLSSRTEQQAGSL 293 S+DE G+LL AL+ MNG+L +IV VR G +TI TAS EIATGNLDLS+RTE QAGSL Sbjct: 237 SSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSL 296 Query: 294 EETAATMEQLTSTVKQNADNARQANQLAATASDVAAHSGSVVAQVVTTMGSISESSRKIV 353 EETA+ MEQLTSTVKQNADNARQAN+LAA+AS VA G+VV QVV TMG+I SS+KI Sbjct: 297 EETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIA 356 Query: 354 DIISVIDGIAFQTNILALNAAVEA--HAPASRAAALRLSPAKCAAWRNARPAR 404 DII VIDGIAFQTNILALNAAVEA R A+ S + A R+A A+ Sbjct: 357 DIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAK 409 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.126 0.338 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 877,857 Number of extensions: 27770 Number of successful extensions: 418 Number of sequences better than 1.0e-08: 67 Number of HSP's gapped: 360 Number of HSP's successfully gapped: 67 Length of query: 442 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 354 Effective length of database: 1,203,190 Effective search space: 425929260 Effective search space used: 425929260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)