BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0012.p (444 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 369 e-103 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 292 4e-80 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 73 6e-14 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 369 bits (947), Expect = e-103, Method: Compositional matrix adjust. Identities = 200/432 (46%), Positives = 265/432 (61%), Gaps = 7/432 (1%) Query: 7 MHLGAFLMS--VGHHAAGWRFPGVDPANTMNFAYYKKLAQIAEAGKFDLIFFADRLAISD 64 MHL AFL++ H A WR P + YY +A+ E GKFDL+FFADRLA+S Sbjct: 1 MHLAAFLIAGNAAHSQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVST 60 Query: 65 RFDNRFDTTVRYLAQ--ARPEPISLLGALGAVTEKIGLAATASTTYNEPFFLARAFATLD 122 R+ + + Q R +P+ +LGAL A T IGL AT STTY++P+ LAR FATLD Sbjct: 61 RYGDSHLHGLYIGDQDATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLD 120 Query: 123 HLTGGRIAWNIVTSTNDGEALNFSKQEHLEHETRYGRAHEFLDVVTRLWDSWEDDALVLD 182 HL+GGR AWN+VTS N GEA NF +E L H+ RY RA EFL+V +LW SW+ DAL L Sbjct: 121 HLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQ 180 Query: 183 KENGVYADPSKVHYLHHKGDWFGVRGPLNVTRPPQGHPVLIQAGSSDTGRDFAAQTAEVI 242 ++ YAD KV + H+G F VRGPLN+ PQGHPV+IQAGSSD G+DFAA+ AEV+ Sbjct: 181 QDR--YADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVV 238 Query: 243 FSSQPQIAAAREFRNDIHARVLKYGRTPDSIKILPGVMPIIGATQAEAEEKLRHLEDLVH 302 F+ QP + + F D+ R ++GR P I+IL VMP +G ++ A E L Sbjct: 239 FNIQPDLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQANALAD 298 Query: 303 PLGGLALLSDSMNHDLSVYSLDEPLPDLKDIRGNQSRYKLVQEMSKRDNLTLRQLGKRFG 362 PL GL+ LS MN D S ++LD PL D++ + G Q +KL++ +S LTL G+ + Sbjct: 299 PLVGLSTLSSHMNVDFSGFALDAPLGDVQ-VSGIQGLFKLLRAISAERQLTLADAGRLYA 357 Query: 363 GSRSHRVVAGTAEQIADNFAEWFDSGACDGFNLMPAYLPESLSDFVEQVVPILQERGLFR 422 +AGTA +AD + DGF + P++LP DFV+QV+P LQ RG FR Sbjct: 358 QGVLTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQVIPELQRRGAFR 417 Query: 423 REYEGDTLRRHL 434 ++Y G LR+HL Sbjct: 418 QDYAGRQLRQHL 429 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 292 bits (748), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 177/451 (39%), Positives = 245/451 (54%), Gaps = 20/451 (4%) Query: 3 SPKQMHLGAFLMS-VGHHAAG-WRFPGVDPANTMNFAYYKKLAQIAEAGKFDLIFFADRL 60 + K++ L AF M+ V H G W P + + AY+ LA++ E GKFD +F AD + Sbjct: 2 AKKEIRLNAFNMNCVSHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIV 61 Query: 61 AISDRFDNRFDTTVRYLAQ-ARPEPISLLGALGAVTEKIGLAATASTTYNEPFFLARAFA 119 + D + D T+R Q +P+ L+ A+ AVTE +G TA+ TY PF AR + Sbjct: 62 GVYDVYQGNTDLTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMS 121 Query: 120 TLDHLTGGRIAWNIVTSTNDGEALNFSKQEHLEHETRYGRAHEFLDVVTRLWD-SWEDDA 178 TLDHL+ GR+ WNIVT D A E LEH+ RY RA EF++VV +LW+ SWED A Sbjct: 122 TLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGA 181 Query: 179 LVLDKENGVYADPSKVHYLHHKGDWFGVRGPLNVTRPP-QGHPVLIQAGSSDTGRDFAAQ 237 ++ D+ VYADP+KVH + H GD++ V G ++++ P Q PVL QAGSS G+ F A+ Sbjct: 182 VLRDRARRVYADPAKVHKIAHHGDYYHVEG-IHLSEPSRQRTPVLYQAGSSGRGQAFGAR 240 Query: 238 TAEVIFSSQPQIAAAREFRNDIHARVLKYGRTPDSIKILPGVMPIIGATQAEAEEKLRHL 297 AE +F S A R+ I ++ GR PD+IKI G+ + G T+ +A+E+ R Sbjct: 241 HAECVFISSQSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERYREY 300 Query: 298 EDLVHPLGGLALLSDSMNHDLSVYSLDEPLPDLKDIRGNQSRYKLVQE----MSKRDNLT 353 P GLA S D S Y LDEP+ K +S K+V + +KR L Sbjct: 301 ARYASPEAGLAHYSAGTGIDFSQYELDEPIRPAK-TNAIESLTKVVTQPELNWTKRKLLQ 359 Query: 354 LRQLGKRFGGSRSHRVVAGTAEQIADNFAEWFDSGACDGFNLMPAYLPESLSDFVEQVVP 413 +LG R+ + G +++AD W DG+NL PES DFVE V+P Sbjct: 360 QLELGGRYAS------IVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIP 413 Query: 414 ILQERGLFRREYEGDTLRRHL---GLARPQN 441 +LQERG ++REY TLR L G PQ Sbjct: 414 VLQERGSYKREYAQGTLREKLFQRGATLPQE 444 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 72.8 bits (177), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 70/266 (26%), Positives = 112/266 (42%), Gaps = 38/266 (14%) Query: 38 YYKKLAQIAEAGKFDLIFFADRLAISDRFDNRFDTTVRYLAQARPEPISLLGALGAVTEK 97 Y ++LA AE F+ R T Y A+ + E +S AL T K Sbjct: 45 YNQRLAVTAEKAGFEYALSQIRF------------TAGYGAEYQHESVSFSQALLHATTK 92 Query: 98 IGLAATASTTYNEPFFLARAFATLDHLTGGRIAWNIVTSTNDGEALNFSKQEHLEHETRY 157 + + A P +A+ AT+DH++ GRIA N+V+ GE + LEH+ RY Sbjct: 93 LKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGE-FQAIGEPWLEHDERY 151 Query: 158 GRAHEFLDVVTRLWDSWEDDALVLDKENGVYADPSKVHYLHHKGDWFGVRGPLNVTRPPQ 217 R++EF+ + +W +DD KGD++ +P Q Sbjct: 152 RRSNEFIRALKGIWT--QDD-------------------FTFKGDFYRFNNYTLSPKPVQ 190 Query: 218 G-HPVLIQAGSSDTGRDFAAQTAEVIFSSQPQIAAAREFRNDIHARVLKYGRTPDSIKIL 276 HP + Q GSS RD AA ++ F++ + + +DI A+ + G ++I Sbjct: 191 KPHPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNGH---KVRIG 247 Query: 277 PGVMPIIGATQAEAEEKLRHLEDLVH 302 I T+ EA+ L+ + D H Sbjct: 248 VNAFVIARDTEEEAQAVLQDIIDHAH 273 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,500,255 Number of extensions: 65454 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 3 Length of query: 444 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 356 Effective length of database: 1,203,190 Effective search space: 428335640 Effective search space used: 428335640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)