BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0006.p (269 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 163 1e-41 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 147 8e-37 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 109 2e-25 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 105 3e-24 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 102 5e-23 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 163 bits (412), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 92/180 (51%), Positives = 119/180 (66%), Gaps = 11/180 (6%) Query: 1 VLKRIAPDQLRLGMYVQEIPGPWMNHPFWRGSFKLEKYEDLKTLRSAKIEQILIDTTKGR 60 +LK IA DQLRLGMY+ IPG W++HPF R SFK+E EDL+TLR I+ I I+T KGR Sbjct: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 Query: 61 DIVMED--EPKAAAAAPATAEIAAETASEATRPKQAKIVVHVGAEQERVQAARVLTGSRR 118 D+ E+ E A APA A + A+R A E+ER Q R+++ S+ Sbjct: 61 DVTREELEEDDADQDAPAAPPADAANRNPASRTDTA-------IERERAQ--RIISSSKS 111 Query: 119 AIMTMFNETRMGKALDIRQAMPVVQEITASVARNSGALISLARLKTADDYTYMHSVXASA 178 ++ MF+E RMGKA+D++ A +V EITASV+RN+ ALISLARLKT DDYTY+HSV A Sbjct: 112 TVLHMFSEARMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCA 171 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 147 bits (372), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 80/178 (44%), Positives = 113/178 (63%), Gaps = 4/178 (2%) Query: 1 VLKRIAPDQLRLGMYVQEIPGPWMNHPFWRGSFKLEKYEDLKTLRSAKIEQILIDTTKGR 60 +LK I P QLR+GMY+Q G W P WR F LEK +DL L+++ I +I+ID KG Sbjct: 1 MLKFITPKQLRIGMYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGA 60 Query: 61 DIVMEDEPKAAAAAPATAEIAAETASEATRPKQAKIVVHVGAEQERVQAARVLTGSRRAI 120 D V DE + ++ AE P+ K + AE+ER AAR+++ S + + Sbjct: 61 D-VQTDEAEQGNGVESSLGKNAEIEVAQALPEAGK-RTDIQAERER--AARIISSSEKTV 116 Query: 121 MTMFNETRMGKALDIRQAMPVVQEITASVARNSGALISLARLKTADDYTYMHSVXASA 178 +TMF++ RMGK +D+++A +V+EI ASV+RN+ A+ISL RLKTAD+YTYMHSV A Sbjct: 117 LTMFSQARMGKTIDVKEASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVAVCA 174 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 109 bits (273), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 62/185 (33%), Positives = 96/185 (51%), Gaps = 10/185 (5%) Query: 1 VLKRIAPDQLRLGMYVQEIPGPWMNHPFWRGSFKLEKYEDLKTLRSAKIEQILIDTTKGR 60 +LK+I + L LGMY W++HPFW+ F L+ +L+ ++ I IDT +G Sbjct: 1 MLKKIKVEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGL 60 Query: 61 DIVMEDEPKAAAAAPATAEIAAETASEATRPKQAKIVVHVGAEQERVQAARVLTGSRRAI 120 D+ E+E +AA E + E A P A ++ A + +++A Sbjct: 61 DVAQEEEAADSAAPLQETEPSPEPEHAAAPPIDAVLL----------DAMALRETAKKAT 110 Query: 121 MTMFNETRMGKALDIRQAMPVVQEITASVARNSGALISLARLKTADDYTYMHSVXASATP 180 MF+E RMGK LD+ + + VV+EI SV ++ +S+ RLK AD+YTY+HSV A Sbjct: 111 KAMFSEARMGKVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALM 170 Query: 181 RSTSR 185 + R Sbjct: 171 VALGR 175 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 105 bits (263), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 66/178 (37%), Positives = 93/178 (52%), Gaps = 13/178 (7%) Query: 1 VLKRIAPDQLRLGMYVQEIPGPWMNHPFWRGSFKLEKYEDLKTLRSAKIEQILIDTTKGR 60 +LK+I L LGMY W+ HPFW+ F L + DL+ R++ I ID ++GR Sbjct: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGR 60 Query: 61 DIVMEDEPKAAAAAPATAEIAAETASEATRPKQAKIVVHVGAEQERVQAARVLTGSRRAI 120 D+ A A A A A A+ S AT H GA+ +R A + +R Sbjct: 61 DVAG----AAPAKAEAAAAPASPDQSAAT---------HAGADADRASAVALREHAREVT 107 Query: 121 MTMFNETRMGKALDIRQAMPVVQEITASVARNSGALISLARLKTADDYTYMHSVXASA 178 MFN RMGK +++ Q + +V+EI SV R+ L+S+ RLK D+YTY+HSV A Sbjct: 108 RQMFNAARMGKMVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCA 165 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 102 bits (253), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 61/173 (35%), Positives = 89/173 (51%), Gaps = 18/173 (10%) Query: 2 LKRIAPDQLRLGMYVQEIPGPWMNHPFWRGSFKLEKYEDLKTLRSAKIEQILIDTTKGRD 61 LK+I QL +GMY G W++ PFWR SF + K ++ R+A IDT +G D Sbjct: 4 LKKIEVGQLSVGMYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQGID 63 Query: 62 IVMEDEPKAAAAAPATAEIAAETASEATRPKQAKIVVHVGAEQERVQAARVLTGSRRAIM 121 + A AE A+ E P+ A + E A R+ +++ Sbjct: 64 L-------------AGAE--ADRLPETEEPEAAG---SSSSRSELDDALRLYENAKQTTQ 105 Query: 122 TMFNETRMGKALDIRQAMPVVQEITASVARNSGALISLARLKTADDYTYMHSV 174 MF + R+GK LD+ +V+EI SVAR+S AL+S+ RLK D+YTY+HS+ Sbjct: 106 QMFKQARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSI 158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.126 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 577,841 Number of extensions: 15480 Number of successful extensions: 70 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 65 Number of HSP's successfully gapped: 5 Length of query: 269 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 185 Effective length of database: 1,222,130 Effective search space: 226094050 Effective search space used: 226094050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)