BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0005.p (410 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 564 e-162 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 233 2e-62 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 127 1e-30 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 109 4e-25 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 107 2e-24 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 564 bits (1454), Expect = e-162, Method: Compositional matrix adjust. Identities = 286/351 (81%), Positives = 312/351 (88%) Query: 1 MEPSKSKAAAAAPVHPVVIAGEGLPADKRAIAIITLLIAVGLATLDTAIANTALPVMARD 60 M + AAP +VIAGEGLP ++R AI+ L IAVGLATLDTAIANTALP MA D Sbjct: 1 MSSQPTAPGTAAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALD 60 Query: 61 LNTTPAASVWIVTAYQLAMMVSLLPLAALGEIVGYRRIYIGGLALFTAASLLCAVAWSLP 120 L TTPAASVWIVTAYQLAMMV+LLPLAALGEI+GYRRIYI GL LFTAASLLCAVAWSLP Sbjct: 61 LQTTPAASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLP 120 Query: 121 ALTVARFLQGLGGAGIMSVNTALIRYIYPTRSLGRGVGINTLIVAISFTIGPSVASGILA 180 L +ARFLQGLGGAGIMSVNTALIR+IYPTRSLGRGVG+NTLIVAI+FT+GPSVASGILA Sbjct: 121 TLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA 180 Query: 181 IAPWPWLFAVNVPLGIIGLVMAVPSLPYTPKSTHHFDIKSALLNAAAFGLLIMAIGEGAH 240 IAPWPWLFAVNVPLG++ +V+AVPSLP+T + H FD++SALLNAAAFGLLI+AIGEGAH Sbjct: 181 IAPWPWLFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAH 240 Query: 241 QAAPSVVVLEFVAALVCGYLLMRREASHPAPMLPVDLFRRPMFSLSAITAVCSFAAQGLA 300 QA SVV LE AA++CG L++RE SHPAPMLPVDLFR PMFSLSA+TAVCSFAAQGLA Sbjct: 241 QAQASVVGLELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLA 300 Query: 301 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDHYPAGMLGG 351 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSD YP GMLGG Sbjct: 301 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGG 351 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 233 bits (594), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 134/331 (40%), Positives = 200/331 (60%), Gaps = 8/331 (2%) Query: 22 EGLPADKRAIAIITLLIAVGLATLDTAIANTALPVMARDLNTTPAASVWIVTAYQLAMMV 81 EGLP +R A++ ++ A +A D I N ALP +++ L T+ A S+W+ AY LA + Sbjct: 20 EGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAM 79 Query: 82 SLLPLAALGEIVGYRRIYIGGLALFTAASLLCAVAWSLPALTVARFLQGLGGAGIMSVNT 141 +L AAL +G+R + GLA+FT ASL CA++ S+ L + R LQGLGGA ++S+ Sbjct: 80 TLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAP 139 Query: 142 ALIRYIYPTRSLGRGVGINTLIVAISFTIGPSVASGILAIAPWPWLFAVNVPLGIIGLVM 201 A+ R I+P R LGR +G+N ++VA S +GP + +LA+ W W+FA+NVPLG++ + + Sbjct: 140 AIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPLGVLAVWL 199 Query: 202 AVPSLPYTPKSTH-HFDIKSALLNAAAFGLLIMAIGEGAHQAAPS-------VVVLEFVA 253 A ++P T K FD+ AL +A A G LIMA A A P V Sbjct: 200 AWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFTAAAYAVT 259 Query: 254 ALVCGYLLMRREASHPAPMLPVDLFRRPMFSLSAITAVCSFAAQGLAFVSLPFFFHHDLG 313 AL+ G +R + P+LP+D+F P FSL+A+T++ SF QG+AFV+LPF F + G Sbjct: 260 ALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQNAYG 319 Query: 314 RSQVEIGLLMTPWPVAVAIMAPIAGRMSDHY 344 + E L TPWP+ + ++AP AGR++D + Sbjct: 320 YTAFESAALFTPWPLGIVLVAPHAGRLADRH 350 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 127 bits (320), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 102/315 (32%), Positives = 151/315 (47%), Gaps = 4/315 (1%) Query: 33 IITLLIAVGLATLDTAIANTALPVMARDLNTTPAASVWIVTAYQLAMMVSLLPLAALGEI 92 ++ L I +A LD + N L +A L+ + VWIV Y L LL AL + Sbjct: 32 LVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALADR 91 Query: 93 VGYRRIYIGGLALFTAASLLCAVAWSLPALTVARFLQGLGGAGIMSVNTALIRYIYP-TR 151 G RR+Y+ GL LF ASLLC A + P L VAR LQGLG A M + +L+ ++Y R Sbjct: 92 FGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYEDDR 151 Query: 152 SLGRGVGINTLIVAISFTIGPSVASGILAIAPWPWLFAVNVPLGIIGLVMAVPSLPYTPK 211 R +G+ + V ++ +GP V ++ W +F +NVP+G++GLVMA +P Sbjct: 152 VRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQVGG 211 Query: 212 STHHFDIKSALLNAAAFGLLIMAIGEGAHQAAPSVVVLEFVA-ALVCGYLLMRREASHPA 270 ++ S L L + +G S +L +A AL G L+R E A Sbjct: 212 HARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRHELRGIA 271 Query: 271 PMLPVDLFRRPMF-SLSAITAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVA 329 P+LP LF P F + + I + +F G F L F G ++ GL + P Sbjct: 272 PLLPKALFATPQFAAANGIGFLINFGVYGNLF-YLALFLQQGRGADALQTGLQLVPMMAV 330 Query: 330 VAIMAPIAGRMSDHY 344 + ++GRMS H+ Sbjct: 331 IFFGNLMSGRMSAHW 345 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 109 bits (272), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 104/337 (30%), Positives = 161/337 (47%), Gaps = 24/337 (7%) Query: 25 PADKRAIAIITLLIAVG--LATLDTAIANTALPVMARDLNTTPAASVWIVTAYQLAMMVS 82 P +RA+ I +A G + TLDT I NTALP MAR L +V AY L M S Sbjct: 48 PVTQRALLWI---VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTM-AS 103 Query: 83 LLPLAA-LGEIVGYRRIYIGGLALFTAASLLCAVAWSLPALTVARFLQGLGGAGIMSVNT 141 L P + L + G RR+Y + LF+ SLLCA+A SLP L AR +QGLGG+ ++ V Sbjct: 104 LTPASGWLADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGR 163 Query: 142 ALIRYIYPTRSLGRGVGINTLIVAISFTIGPSVASGILAIAPWPWLFAVNVPLGIIGLVM 201 + P + ++ + +GP + ++ W W+F +N+P+G++G+ Sbjct: 164 LAVLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGA 223 Query: 202 AVPSLPYTP-KSTHHFDIKSALLNAA---AFGLLIMAIGEGAHQAAPSVVVLEFVAALVC 257 + LP T FD+ +L +A AF L + + +H+ S+ + A V Sbjct: 224 VLYFLPDDSLAGTPRFDLLGCVLLSACMVAFS-LALDVPFDSHRTGWSIALFVVAALTVL 282 Query: 258 GYLLMRREASHPAPMLPVDLFRRPMFSLS-AITAVCSFAAQGLAFVSLPFFFHHDLGRSQ 316 GY R + HP P+ + LF P FS+ A VC + + F+ LG S Sbjct: 283 GY--WRHASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLP-LLLQVRLGYSP 339 Query: 317 VEIGLLMTPWPVAVAIMAPIAGRMSDHYPAGMLGGFG 353 + G+++ P A IAG ++ + A ++ FG Sbjct: 340 LHAGMVLLP--------AAIAGTIAKPWIAPLVKRFG 368 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 85/310 (27%), Positives = 153/310 (49%), Gaps = 10/310 (3%) Query: 38 IAVGLATLDTAIANTALPVMARDLNTTPAASVWIVTAYQLAMMVSLLPLAALGEIVGYRR 97 +AV + LD++IAN A+P ++ +L + W++T++ A +S+ L + +G R Sbjct: 31 LAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVR 90 Query: 98 IYIGGLALFTAASLLCAVAWSLPALTVARFLQGLGGAGIMSVNTALIRYIYPTRSLGRGV 157 +++ + LF AS LC +A +LP L AR LQGL ++ ++ +L+ YP + Sbjct: 91 LFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFAL 150 Query: 158 GINTLIVAISFTIGPSVASGILAIAPWPWLFAVNVPLGII--GLVMAVPSLPYTPKSTHH 215 + + ++ GP++ I WPW+F +N+P+G+I G+ +A+ TP TH Sbjct: 151 ALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETP--THK 208 Query: 216 FDIKS---ALL--NAAAFGLLIMAIGEGAHQAAPSVVVLEFVAALVCGYLLMRREASHPA 270 I ALL AAF +++ + A+P++V L +A + Y ++ E ++ Sbjct: 209 LPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVV-WELNNDH 267 Query: 271 PMLPVDLFRRPMFSLSAITAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVAV 330 P++ + LF R F+ I + V LP + LG ++ GL P + Sbjct: 268 PVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPIGIFA 327 Query: 331 AIMAPIAGRM 340 I+ P+ GR Sbjct: 328 VILMPLIGRF 337 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,227,407 Number of extensions: 52506 Number of successful extensions: 367 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 352 Number of HSP's successfully gapped: 17 Length of query: 410 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 323 Effective length of database: 1,207,925 Effective search space: 390159775 Effective search space used: 390159775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)