BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0003.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314018|ref|YP_003778110.1| two component sensor histidine ... 506 e-145 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 93 3e-20 gi|300311808|ref|YP_003775900.1| two component sensor histidine ... 90 2e-19 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 90 3e-19 gi|300311751|ref|YP_003775843.1| signal transduction histidine k... 85 7e-18 >gi|300314018|ref|YP_003778110.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 492 Score = 506 bits (1304), Expect = e-145, Method: Compositional matrix adjust. Identities = 250/308 (81%), Positives = 276/308 (89%) Query: 1 LSNDLEQQRQMLTFQVIGAIVLAVLLAFWFGLWLSRPLARIELAINRLGENRFDQPIIVK 60 LS LEQQRQMLT QV+GAIVLAVLLAF FGLWLSRP+AR+E AI RLG+NRFDQPI V+ Sbjct: 185 LSAALEQQRQMLTAQVVGAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRFDQPIDVR 244 Query: 61 GPADLRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDEVAGKLT 120 GPAD+RRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDD VAG+LT Sbjct: 245 GPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLT 304 Query: 121 DSQREISRILRQNTQALQNQIEDLLRYNEVAFDAQHINRLPVDLRALLYKVIDDQRLQWT 180 DSQREI+RILRQNTQ+LQ QIEDLLRYNEV+FDAQ I+ +PVDLRALL+KVIDDQRLQW Sbjct: 305 DSQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQRLQWL 364 Query: 181 ARDLKVEVEGGARTAVLDPDKFAIVLANLLSNAVRFSPRGGTIRFVLSGHEGRVRIDCID 240 AR+LKVE+EG ART V+DP+K AIVLANLLSNAVRFSP+GG IRF+LS G VR++C+D Sbjct: 365 ARELKVEIEGAARTVVVDPEKMAIVLANLLSNAVRFSPQGGFIRFLLSEAPGLVRVECVD 424 Query: 241 QGPGVAATDTVRIFDPFYQGLNQPQGARHGNGIGLSIVREYVLAHNGEVQLVAGPGGAHF 300 QGPGVA TD RIF+PFYQGLNQP AR GNGIGLS+VREYV AH+G+V LV GGAHF Sbjct: 425 QGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVPREGGAHF 484 Query: 301 RIELPDES 308 RIELPDE Sbjct: 485 RIELPDEK 492 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 86/288 (29%), Positives = 133/288 (46%), Gaps = 27/288 (9%) Query: 21 VLAVLLAFWFGLWLS-RPLARIELAINRLGENRFDQPIIVKGPADLRRLGQQLDWLRQRL 79 + AV+L W + L+ +PL + A +LG N P+ GP ++ Q + +++R+ Sbjct: 211 IFAVVLIAWMAVRLAMKPLQVMAEAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQRRI 270 Query: 80 ADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDEVAG-KLTDSQREISRILRQNTQALQ 138 D AE++RFL VSH+L++P+ + +L DE K E+ ++ A Sbjct: 271 IDNIAERTRFLAAVSHDLRSPITRMKLRTEMLPDERGKEKFRKDLDEMDALI----SATL 326 Query: 139 NQIEDLLRYNEVAFDAQHINRLPVDLRALLYKVIDDQRLQWTARDLKVEVEGGARTAVLD 198 N ++D+ D Q +L +DL +LL + DQ ++ EV A L Sbjct: 327 NVVQDI--------DVQERPQL-IDLDSLLESLRTDQ------AEVGGEVTLSGHAAPLQ 371 Query: 199 --PDKFAIVLANLLSNAVRFSPRGGTIRFVLSGHEGRVRIDCIDQGPGVAATDTVRIFDP 256 P L NL+ NAVR+ R R L +V I D GPG+ R+ +P Sbjct: 372 GYPRSLRRCLQNLVENAVRYGQRA---RVQLQDDADQVCIRVDDDGPGIPEALLERVQEP 428 Query: 257 FYQGLNQPQGARHGNGIGLSIVREYVLAHNGEVQLVAGPGGAHFRIEL 304 FY+ G G+GLSI V AH G ++LV PGG R+E+ Sbjct: 429 FYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGG-LRVEI 475 >gi|300311808|ref|YP_003775900.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 289 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 70/229 (30%), Positives = 111/229 (48%), Gaps = 14/229 (6%) Query: 86 KSRFLRHVSHELKTPLAALCEGAA-LLDDEVAGKLTDSQREIS--RILRQNTQALQNQIE 142 K FL +SHEL+TPL A+ LL+D +D+ R + +++ +N + +E Sbjct: 50 KDEFLATLSHELRTPLNAIVGWTQYLLNDH-----SDADRLVKGLQVIERNARLQAQMVE 104 Query: 143 DLLRYNEVAFDAQHINRLPVDLRALLYKVIDDQRLQWTARDLKVEVEGGARTAVL-DPDK 201 DLL + + + P+DL ++ + I R AR +++ G +L DP + Sbjct: 105 DLLDMSRMLAGKLRLETRPMDLASVAEEAIAAVRPAAEARQIELHPALGQGGVILGDPAR 164 Query: 202 FAIVLANLLSNAVRFSPRGGTIRFVLSGHEGRVRIDCIDQGPGVAATDTVRIFDPFYQ-- 259 V+ NLL NA++F+PR G + L H+ V ++ D G G+ +FD F Q Sbjct: 165 LQQVVWNLLMNAIKFTPRQGRVSLSLRQHDDTVALEVSDNGRGIKGEFLGSVFDRFTQED 224 Query: 260 -GLNQPQGARHGNGIGLSIVREYVLAHNGEVQLV-AGPG-GAHFRIELP 305 G G+GL+I R V H G+++ AGPG GA F + LP Sbjct: 225 VSTGSTGSTASGLGLGLAIARHLVELHGGQIRAASAGPGMGAAFTVTLP 273 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 89.7 bits (221), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 85/307 (27%), Positives = 133/307 (43%), Gaps = 9/307 (2%) Query: 4 DLEQQRQMLTFQVIGAIVLAVLLAFW---FGLWLSRPLARIELAINRLGENRFDQPIIVK 60 DL Q+ Q+I A+V +L AF L+RPL R+ A +RL + + V+ Sbjct: 163 DLRFQQSQFAAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVE 222 Query: 61 GPADLRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDEVAGKLT 120 ++ L Q + L L E + F+ VSHEL+TPLA L L+D V Sbjct: 223 SGDEVGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGV----L 278 Query: 121 DSQREISRILRQNTQALQNQIEDLLRYNEVAFDAQHINRLPVDLRALLYKVIDDQRLQWT 180 + R L+ + L + DL + A R +DL LL +++ Sbjct: 279 QPDAQTLRSLKSEVEHLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFS 338 Query: 181 ARDLKVEVEGGARTAVL-DPDKFAIVLANLLSNAVRFSPRGGTIRFVLSGHEGRVRIDCI 239 R +++ E A V D + +L+NL N++R++ GG ++ + + Sbjct: 339 QRGMRLSCELPASMPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFD 398 Query: 240 DQGPGVAATDTVRIFDPFYQGLNQPQGARHGNGIGLSIVREYVLAHNGEVQLVAGP-GGA 298 D PGV R+F+ F++ A G G+GL+I R V AH G + A P GG Sbjct: 399 DSEPGVPPEVMARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGL 458 Query: 299 HFRIELP 305 + LP Sbjct: 459 RVVVVLP 465 >gi|300311751|ref|YP_003775843.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 85.1 bits (209), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 74/251 (29%), Positives = 121/251 (48%), Gaps = 21/251 (8%) Query: 37 PLARIELAINRLGENRFDQPIIVKG-PADLRRLGQQLDWLRQRLADLEAEKSRFLRHVSH 95 PL + I+ GE+ QPI + P +++ ++ L R+ A + RF+ +H Sbjct: 179 PLQALRQEIDSRGEDDL-QPIAERLLPREVQAFVAAINGLLARVEAGMAAQRRFVADAAH 237 Query: 96 ELKTPLAALCEGAALLDDEVAGKLTDSQREISRILR-----QNTQALQNQIEDLLRYNEV 150 EL+TPL AL L E G+ S+ R+ R Q + L Q+ L R Sbjct: 238 ELRTPLTALS-----LQAERLGRAPMSETARERLTRLETGLQRARQLVTQLLALARAQTP 292 Query: 151 AFDAQHINRLPVDLRALLYKVIDDQRLQWTARDLKVEVEGGARTAVL-DPDKFAIVLANL 209 A A P L +L +VI++ + + + ++G VL P + A +L NL Sbjct: 293 AGAALQ----PCSLHQVLREVIEELLPLAQCKQIDLGLDGERDACVLTQPQELAQLLRNL 348 Query: 210 LSNAVRFSPRGGTIRFVLSGHEGRVRIDCIDQGPGVAATDTVRIFDPFYQGLNQPQGARH 269 + NA+ ++P GG + + H V ++ D GPG+AA++ R+F PFY+ P G Sbjct: 349 IDNAIAYTPAGGAVSLRMERHAQWVALEICDTGPGIAASERERVFQPFYR--IDPSG--Q 404 Query: 270 GNGIGLSIVRE 280 G+G+GL+IV+ Sbjct: 405 GSGLGLAIVQS 415 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.140 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,029,336 Number of extensions: 45332 Number of successful extensions: 313 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 38 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)