BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0001.p (224 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314016|ref|YP_003778108.1| two component transcription reg... 251 2e-68 gi|300312425|ref|YP_003776517.1| nitrogen assimilation transcrip... 124 4e-30 gi|300313916|ref|YP_003778008.1| two component response regulato... 115 4e-27 gi|300312654|ref|YP_003776746.1| C4-dicarboxylate transport resp... 110 8e-26 gi|300312042|ref|YP_003776134.1| response regulatory protein [He... 90 1e-19 >gi|300314016|ref|YP_003778108.1| two component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 466 Score = 251 bits (642), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 132/199 (66%), Positives = 157/199 (78%) Query: 25 TANGYRVTGVASTEAAQAQLAMDLPALVISDIRLPGKDGLALFDEIRAQYPALPVILLTA 84 A+GYR+ V S EAA AQLA+ LPALVI+D+RLPG DGLALF EIR++YPALPVILLTA Sbjct: 31 NASGYRILAVESAEAALAQLAISLPALVITDVRLPGMDGLALFAEIRSRYPALPVILLTA 90 Query: 85 HGTIPDAVEATTRGAFAYLTKPYDPKILLAKIEQALSLTASVDGNADEAWREELISRSPR 144 HGTIPDAV+ATT+GAFAYLTKP+D +LL K+ QALSL A + D++WR +++SRS R Sbjct: 91 HGTIPDAVDATTQGAFAYLTKPFDGHVLLDKVAQALSLAAPAPLSPDQSWRADILSRSQR 150 Query: 145 MAEVLSEARLVAVSDASILIRGESGTGKELLARAIHRASRRAKAPFIAVNCAAYYIGILP 204 +AE+L+EARLVA SDASILIRG+SGTGKELLARAIHRAS RA+ PFIAVNC A +L Sbjct: 151 VAELLAEARLVAASDASILIRGDSGTGKELLARAIHRASARAEQPFIAVNCGAIPEQLLE 210 Query: 205 AIKGGGRTTGARAAVESRE 223 + G AVE RE Sbjct: 211 SELFGHVKGAFTGAVEHRE 229 >gi|300312425|ref|YP_003776517.1| nitrogen assimilation transcription activator protein [Herbaspirillum seropedicae SmR1] Length = 500 Score = 124 bits (312), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 79/220 (35%), Positives = 119/220 (54%), Gaps = 4/220 (1%) Query: 7 LLLVDDDADLLQLLSLRLTANGYRVTGVASTEAAQAQLAMDLPALVISDIRLPGKDGLAL 66 + +VDDD + +L L +S A A L P +++SDIR+PG GL L Sbjct: 4 IWIVDDDESIRWVLEKALARENLATQSFSSARDAIAALQNGTPQVLVSDIRMPGASGLEL 63 Query: 67 FDEIRAQYPALPVILLTAHGTIPDAVEATTRGAFAYLTKPYDPKILLAKIEQALSLT-AS 125 ++A++P +PVI++TA + AV A GAF YL KP+D + I +AL + Sbjct: 64 LQTVKAKHPGIPVIIITAFSDLDSAVSAFQGGAFEYLAKPFDVDKAVELIRRALDESLRE 123 Query: 126 VDGNADEAWREELISRSPRMAEVLSEARLVAVSDASILIRGESGTGKELLARAIHRASRR 185 D A E++ ++P M +V ++ S+ ++LI GESG+GKEL+ARA+H+ S R Sbjct: 124 TDIEQGAAQTPEILGQAPAMQDVFRAIGRLSQSNVTVLITGESGSGKELVARALHKHSPR 183 Query: 186 AKAPFIAVNCAAYYIGILPAIKGG---GRTTGARAAVESR 222 A PF+A+N AA +L + G G TGA+A R Sbjct: 184 ASQPFVALNTAAIPKDLLESELFGHERGAFTGAQAMRRGR 223 >gi|300313916|ref|YP_003778008.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 448 Score = 115 bits (287), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 75/193 (38%), Positives = 107/193 (55%), Gaps = 3/193 (1%) Query: 7 LLLVDDDADLLQLLSLRLTANGYRVTGVASTEAAQAQLAMDLPALVISDIRLPGKDGLAL 66 +L+VDDDA L+ ++ G+ V ST A LA I D+R+P +DGLA Sbjct: 4 ILIVDDDAAFRGSLAETVSDLGHDVLEAGSTAAGLKLLAAHAVDAAIVDLRMPDEDGLAF 63 Query: 67 FDEIRAQYPALPVILLTAHGTIPDAVEATTRGAFAYLTKPYDPKILLAKIEQAL--SLTA 124 A A+P I+LTA+ + + +EA GAF +LTKP L+ +E+AL + Sbjct: 64 LQRAPA-IAAIPCIMLTAYASGDNTIEAIRLGAFDHLTKPVPRAALVETLERALRQGAST 122 Query: 125 SVDGNADEAWREELISRSPRMAEVLSEARLVAVSDASILIRGESGTGKELLARAIHRASR 184 + R +L+ S M +V + A SDA++L+ GE+GTGKEL+ARA+HR Sbjct: 123 PAAASPATEERNQLVGESEAMRQVFKQIGRAATSDATVLVLGETGTGKELVARALHRNGA 182 Query: 185 RAKAPFIAVNCAA 197 RA PF+AVNCAA Sbjct: 183 RAARPFVAVNCAA 195 >gi|300312654|ref|YP_003776746.1| C4-dicarboxylate transport response regulator transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 110 bits (275), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 71/200 (35%), Positives = 110/200 (55%), Gaps = 14/200 (7%) Query: 8 LLVDDDADLLQLLSLRLTANGYRVTGVASTEAAQAQLAMDLPALVISDIRLPGKDGLALF 67 LL++D+ + + + L G+ VT S E A + D +V+SD+RLPG GL + Sbjct: 11 LLIEDEQTVRRATAQALELGGFAVTACGSAEEALPLITRDFAGVVVSDVRLPGLSGLDVL 70 Query: 68 DEIRAQYPALPVILLTAHGTIPDAVEATTRGAFAYLTKPYDPKILL-----AKIEQALSL 122 + A LPVI++T HG + AVEA GA+ ++ KP+ LL A+ ++ L L Sbjct: 71 ARVVAIDHDLPVIVVTGHGDVGMAVEAMRAGAYDFVEKPFSSDTLLEIALRAQEKRNLVL 130 Query: 123 TASVDGNADEAWREE-----LISRSPRMAEVLSEARLVAVSDASILIRGESGTGKELLAR 177 + EAW + L+ +SP + V + R + +D +LI G++GTGKE++AR Sbjct: 131 E---NRRLREAWAHDPELPPLVGQSPAIERVRTLIRSLGPADVDVLINGQTGTGKEVVAR 187 Query: 178 AIHRASRRAKAPFIAVNCAA 197 +H AS R K PF+A+NC A Sbjct: 188 QLHAASGR-KGPFVALNCGA 206 >gi|300312042|ref|YP_003776134.1| response regulatory protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 90.1 bits (222), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 70/202 (34%), Positives = 103/202 (50%), Gaps = 13/202 (6%) Query: 7 LLLVDDDADLLQLLSLRLTANGYRVTGVASTEAAQAQLAMDLPALVISDIRLP-----GK 61 LL+V+DD L + LR + + Y V E+A A + PA+V D+ LP Sbjct: 8 LLIVEDDPGLQK--QLRWSLDAYEVLVAGDRESALAMVRRHEPAVVTMDLGLPPDPDGAS 65 Query: 62 DGLALFDEIRAQYPALPVILLTAHGTIPDAVEATTRGAFAYLTKPYDPKILLAKIEQALS 121 +G A ++ A P VI+LT + AV+A + GA+ + KP + L +++A Sbjct: 66 EGFATLQQVLALAPDTKVIMLTGNQDHAHAVKAISMGAYDFHQKPCIDETLRLVVQRAFY 125 Query: 122 LTASVDGNA--DEAWREE----LISRSPRMAEVLSEARLVAVSDASILIRGESGTGKELL 175 L A N +A LI+R M V VA S AS+++ G SGTGKE+L Sbjct: 126 LHALQRENQRMQQAGGHTALGGLITRDAGMLAVCRSIEKVAPSSASVMLLGSSGTGKEVL 185 Query: 176 ARAIHRASRRAKAPFIAVNCAA 197 ARA+H+ S R+ F+A+NCAA Sbjct: 186 ARALHQLSPRSGRRFMAINCAA 207 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 577,930 Number of extensions: 21845 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 253 Number of HSP's successfully gapped: 41 Length of query: 224 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 142 Effective length of database: 1,231,600 Effective search space: 174887200 Effective search space used: 174887200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)