BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0007.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 682 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 281 8e-77 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 206 2e-54 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 146 3e-36 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 122 6e-29 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 682 bits (1759), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/386 (86%), Positives = 358/386 (92%) Query: 1 MDFELNEDQLAFQASARDFAAGEMAPHAARWDEESHFPVDVIAKAGELGFCALYTPEEVG 60 MDFELN+DQLAFQ SARDFAAGEMAP AA+WDEE+HFP+DVIAKAGELGFC LYTPE G Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 61 GLGLSRLDATVVFEELAYACPSTAAFLTIHNMVSWMIASWATPEVKATWCGDLAAGTKIG 120 GLGLSRLDATVVFEELA AC STAA+LTIHNMVSWMIASW P +KA WC LAAG KIG Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGRKIG 120 Query: 121 SYCLTEPGSGSDAASLKTTAKLVDGHYIINGSKAFISGAGATDVLVLMARTGGDGAKGVS 180 SYCLTEPGSGSDAASLKTTA+L DGHY++NGSKAFISGAG+TDVLV+MARTGG+GA+GVS Sbjct: 121 SYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVS 180 Query: 181 AIVVPANTPGISYGKKEVKMGWNSQPTRTISFDNVKVPVNHLLGAEGEGFKFAMKGLDGG 240 AIVVP N PG+SYGKKE KMGWNSQPTRTISFDNVKVP +HLLG EG+GF FAMKGLDGG Sbjct: 181 AIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGG 240 Query: 241 RINIATCSVGAAQAALDAAHAYMKERKQFGRPLADFQALQFKLADMQTELVAARQMVRLA 300 RINIATCSVGAAQAALDAAHAYMKERKQFGRP+ADFQALQFKLADMQTELVAARQMVRLA Sbjct: 241 RINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLA 300 Query: 301 AVKLDSKSPNATTYCAMAKRFATDLGFKICNEALQIHGGYGYIREYPLERYFRDVRVHQI 360 A KLD+ SP ATTYCAMAKR ATDLGF++CNEALQ+HGGYGYIREYPLERYFRDVRVHQI Sbjct: 301 ATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQI 360 Query: 361 LEGTNEIMRVIIARHLLNNDSNEDIR 386 LEGTNEIMRVII+R LL+ D+ +DIR Sbjct: 361 LEGTNEIMRVIISRKLLDRDNLDDIR 386 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 281 bits (718), Expect = 8e-77, Method: Compositional matrix adjust. Identities = 149/380 (39%), Positives = 225/380 (59%), Gaps = 3/380 (0%) Query: 5 LNEDQLAFQASARDFAAGEMAPHAARWDEESHFPVDVIAKAGELGFCALYTPEEVGGLGL 64 L+ + + + R FA + P AA WD FP + + + ELG + PE+ GG G+ Sbjct: 3 LSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGM 62 Query: 65 SRLDATVVFEELAYACPSTAAFLTIHNMVSWMIAS-WATPEVKATWCGDLAAGTKIGSYC 123 + + EE+A +T+ +++ N ++ I + +P K W LA G +G +C Sbjct: 63 DYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFC 122 Query: 124 LTEPGSGSDAASLKTTAKLVDGHYIINGSKAFISGAGATDVLVLMARTGGD-GAKGVSAI 182 LTEP +GSDAA+++T A+ +I+NG+K FI+ V ++ A T G KG+S Sbjct: 123 LTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCF 182 Query: 183 VVPANTPGISYGKKEVKMGWNSQPTRTISFDNVKVPVNHLLGAEGEGFKFAMKGLDGGRI 242 +VP +TPG G+ E KMG ++ T I DN +VP LLG EGEG++ A+ L+ GRI Sbjct: 183 LVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRI 242 Query: 243 NIATCSVGAAQAALDAAHAYMKERKQFGRPLADFQALQFKLADMQTELVAARQMVRLAAV 302 IA SVG A+AA +AA +Y ++R+ FG P+ + QA+ F+LADM T L AAR MV AA Sbjct: 243 GIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQ 302 Query: 303 KLDSKSPNATTYCAMAKRFATDLGFKICNEALQIHGGYGYIREYPLERYFRDVRVHQILE 362 D P +MAK FA++ KI ++A+QIHGG GY ++P+ER +RDVR+ QI E Sbjct: 303 LKDQGRP-CLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYE 361 Query: 363 GTNEIMRVIIARHLLNNDSN 382 G N+I R++I R + ++ ++ Sbjct: 362 GANDIQRLVIGRAIASDQAH 381 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 206 bits (525), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 130/385 (33%), Positives = 199/385 (51%), Gaps = 9/385 (2%) Query: 1 MDFELNEDQLAFQASARDFAAGEMAPHAARWDEESHFPVDVIAKAGELGFCALYTPEEVG 60 + F+ ED A + + FA E+AP AA D FP+D+ K G+LG + EE G Sbjct: 7 LSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYG 66 Query: 61 GLGLSRLDATVVFEELAYACPSTAAFLTIH-NMVSWMIASWATPEVKATWCGDLAAGTKI 119 G GL L + EE++ A S H N+ I E K + L +G I Sbjct: 67 GAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFI 126 Query: 120 GSYCLTEPGSGSDAASLKTTAKLVDGHYIINGSKAFISGAGATDVLVLMARTGGD-GAKG 178 G+ ++EP +GSD S+K A Y++NGSK +I+ DVLV+ A+T + GA+G Sbjct: 127 GALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARG 186 Query: 179 VSAIVVPANTPGISYGKKEVKMGWNSQPTRTISFDNVKVPVNHLLGAEGEGFKFAMKGLD 238 ++A +V G S +K K+G T + F + +VP ++LG G G M GLD Sbjct: 187 MTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLD 246 Query: 239 GGRINIATCSVGAAQAALDAAHAYMKERKQFGRPLADFQALQFKLADMQTELVAARQMVR 298 R ++ +G QA +D Y+ +RKQFG+ + +FQ +Q KLADM + ++A + V Sbjct: 247 FERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVY 306 Query: 299 LAAVKLDSKSPNATTYCAMAKRFATDLGFK------ICNEALQIHGGYGYIREYPLERYF 352 D ++ +A A+ K A + + + EA+Q GG GYI EYP+ R + Sbjct: 307 AVGQACD-RADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLW 365 Query: 353 RDVRVHQILEGTNEIMRVIIARHLL 377 RD ++++I GT+EI R++I R L Sbjct: 366 RDAKLYEIGAGTSEIRRMLIGRELF 390 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 146 bits (368), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 118/375 (31%), Positives = 190/375 (50%), Gaps = 20/375 (5%) Query: 15 SARDFAAGEMAPHAARWDEESHFP---VDVIAKAGELGFCALYTPEEVGGLGLSRLDATV 71 + RD + + DE +P VD + KAG L A P+E GG GL +A+V Sbjct: 16 AVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWL---AALIPQEYGGSGLGLTEASV 72 Query: 72 VFEELAYAC-PSTAAFLTIHNMVSWMIASWATPEVKATWCGDLAAGT-KIGSYCLTEPGS 129 + EE+ S A ++NM + + +PE K + +A+G ++ S +TEP + Sbjct: 73 IMEEINRTGGNSGACHGQMYNMGTLL--RHGSPEQKERYLPRIASGELRLQSMAVTEPTT 130 Query: 130 GSDAASLKTTAKLVDGHYIINGSKAFISGAGATDVLVLMARTG-----GDGAKGVSAIVV 184 G+D +KT A Y+ING K +IS +D+++L+ART ++G+S +V Sbjct: 131 GTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIFLV 190 Query: 185 PANTPGISYGKKEVKMGWN--SQPTRTISFDNVKVPVNHLLGAEGEGFKFAMKGLDGGRI 242 I +G EV+ N + T + FDN+++P +L+G EG GF++ + GL+ R Sbjct: 191 DLKE-AIGHGM-EVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAERT 248 Query: 243 NIATCSVGAAQAALDAAHAYMKERKQFGRPLADFQALQFKLADMQTELVAARQMVRLAAV 302 IA +G +D A Y +ER F RP+ Q +QF LA+ E+ AA ++R A Sbjct: 249 LIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAA-NLMRYQAC 307 Query: 303 KLDSKSPNATTYCAMAKRFATDLGFKICNEALQIHGGYGYIREYPLERYFRDVRVHQILE 362 L + MAK A ++ N LQ HGG+G+ EY +ER FR+ R++Q+ Sbjct: 308 ALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRETRLYQVAP 367 Query: 363 GTNEIMRVIIARHLL 377 + ++ +A H+L Sbjct: 368 ISTNLIFSYVAEHVL 382 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 122 bits (305), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 104/368 (28%), Positives = 171/368 (46%), Gaps = 37/368 (10%) Query: 49 GFCALYTPEEVGGLGLSRLDATVVFEELAYACPSTAAFLTIHNMVSWMIASWATPEVKAT 108 G+ AL E GG GL + ++E L+ A + + + +A+ + ++A Sbjct: 91 GWPALACAPEHGGQGLPHVLDCALYEMLSAANHGWTMYPGLLHGAYACLAAHGSAALQAR 150 Query: 109 WCGDLAAGTKIGSYCLTEPGSGSDAASLKTTA-KLVDGHYIINGSKAFISGAGATD---- 163 + +A+G + + CLTE +GSD L+T A DG Y ++G K FISG G D Sbjct: 151 YLDKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYRLSGDKIFISG-GEHDLTPN 209 Query: 164 -VLVLMART--GGDGAKGVSAIVVP-----ANTPGISYGKKEVKMGWNSQPTRTISFDNV 215 V +++AR G+KG+S + P + G+ E KMG + T ++ F+ Sbjct: 210 IVHLVLARLPDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQA 269 Query: 216 KVPVNHLLGAEGEGFKFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQFGRP--- 272 + L+G G G ++ R+++ +G A+AA A AY +ER+Q P Sbjct: 270 QA---WLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRAPGSS 326 Query: 273 ----LADFQALQFKLADMQTELVAARQMVRLAAVKLD--SKSPNATTYCAMAKR------ 320 + A+Q L + + + AR + A + LD P A +R Sbjct: 327 GNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITP 386 Query: 321 ----FATDLGFKICNEALQIHGGYGYIREYPLERYFRDVRVHQILEGTNEIMRV-IIARH 375 F TD GF+ + ALQ+ GG+GY+ E +E++ RD R+ I EGTNEI + + R Sbjct: 387 VIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRK 446 Query: 376 LLNNDSNE 383 +L ++ Sbjct: 447 VLGDEGRR 454 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,202,473 Number of extensions: 50591 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 13 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)