BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0088.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 529 e-152 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 219 3e-58 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 199 3e-52 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 120 2e-28 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 91 1e-19 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 529 bits (1363), Expect = e-152, Method: Compositional matrix adjust. Identities = 267/299 (89%), Positives = 277/299 (92%) Query: 1 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAVDFVTKPKISIQSGMQEYTE 60 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGA+DFVTKPKISIQSGMQEY + Sbjct: 61 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYAD 120 Query: 61 MIADKIRAASKASIRPRTIPHPDAPKASGDGPLPQMRNPLLSSEKLIIIGASTGGTEAIK 120 MIADKIRAA+KA +R R + G LPQ+RNPL SSEKLIIIGASTGGTEAIK Sbjct: 121 MIADKIRAAAKARVRARPARSAEQAAGGGSEALPQIRNPLTSSEKLIIIGASTGGTEAIK 180 Query: 121 EFLIRMPSDCPGILITQHMPEGFTRSFAQRLDNLCKISVKEAAGNERVLPGHAYIAPGHS 180 EFLIRMPSDCPGILITQHMPEGFTRSFAQRL+NLCKISV E+AGNERVLPGHAYIAPGHS Sbjct: 181 EFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGHS 240 Query: 181 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFNSAAACAGKNAVGVILTGMGKDGALGML 240 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLF SAA CAGKNAVGVILTGMGKDGA GML Sbjct: 241 HLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGML 300 Query: 241 EMRNAGAYNFAQDEASCVVFGMPREAIAVGGAHEVLALQAMPGRVLSYLAEHGSRALRV 299 EMRNAGAYNFAQDEASCVVFGMPREAIAVG AHEVLALQAMPGRVL++LAEHGSRALRV Sbjct: 301 EMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLAEHGSRALRV 359 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 219 bits (557), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 127/317 (40%), Positives = 178/317 (56%), Gaps = 32/317 (10%) Query: 1 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAVDFVTKPKISIQSGMQEYTE 60 MDG+ FL ++M RP PV+M S+ + + AL GAV + +P++ ++ +Q+ E Sbjct: 65 MDGVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVE 124 Query: 61 MIADKIRAASKASIRPRTIPHPDAPKASGDGPLPQMRNPLL------------------- 101 +A IRAA +A R P AP G P+ +L Sbjct: 125 ELASAIRAAMQAREGQRA---PKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQ 181 Query: 102 ----------SSEKLIIIGASTGGTEAIKEFLIRMPSDCPGILITQHMPEGFTRSFAQRL 151 ++ +++ IGASTGG +A++ L ++ CPGI++ QHMP+ FTRSFA+RL Sbjct: 182 PPGPEDGLRPATARIVAIGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERL 241 Query: 152 DNLCKISVKEAAGNERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVL 211 + VKEA +R+LPG IAPG HL + R G Y + GP V+RH+PSVDVL Sbjct: 242 HRISGAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVL 301 Query: 212 FNSAAACAGKNAVGVILTGMGKDGALGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGG 271 F S A AG NAVG+I+TGMG DGA GM E+ + G +AQDEAS VVFGMP+EAI GG Sbjct: 302 FRSLAKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGG 361 Query: 272 AHEVLALQAMPGRVLSY 288 +V+ L+ + + Y Sbjct: 362 VGDVITLEHISAVIEQY 378 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 199 bits (505), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 119/281 (42%), Positives = 164/281 (58%), Gaps = 6/281 (2%) Query: 1 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAVDFVTKPKISIQSGMQEYTE 60 MDGL L +M RP+PVVMVSSLTE+G+ T A+ LGAVD+V KP +I + E + Sbjct: 59 MDGLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQ 118 Query: 61 MIADKIRAASKASIRPRTIPHPDAPKASGDGPLP---QMRNPLLSSEKLIIIGASTGGTE 117 + K+R A+KA IR R P P+ +A P R +S+ L++IG STGG Sbjct: 119 DLLAKVRVAAKARIRSRA-PAPELARAREPLAAPVAEAARRGRPASDGLVLIGVSTGGPS 177 Query: 118 AIKEFLIRMPSDCPG-ILITQHMPEGFTRSFAQRLDNLCKISVKEAAGNERVLPGHAYIA 176 ++E L +P+ P IL+ QHMP FTRSFA+R+D LC ++V E A V GH YIA Sbjct: 178 TLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIA 237 Query: 177 PGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFNSA-AACAGKNAVGVILTGMGKDG 235 G + + + + G++ P + PSV++L SA + A + +GV+LTGMG DG Sbjct: 238 RGGADMLVAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLIGVMLTGMGNDG 297 Query: 236 ALGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGGAHEVL 276 A E+ G A+ E+SCVVFGMP++ I GGA VL Sbjct: 298 AAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVL 338 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 89/293 (30%), Positives = 145/293 (49%), Gaps = 14/293 (4%) Query: 1 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAVDFVTKPKISIQSGMQEYTE 60 +DG + ++M P P+++V+S + ++ A+ GA+D P + +G ++ Sbjct: 59 VDGAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAA 118 Query: 61 MIADKIRAASKASIRPRTIPHP-DAPKASGDGPLPQMRNPLLSSEKLIIIGASTGGTEAI 119 +I +KI + S P + P +A P R L L+ +GAS GG A+ Sbjct: 119 LI-EKIDNLGRLS--PEMVSPPVRGGRAETLAPAQPARADSLPP--LVAVGASAGGPAAL 173 Query: 120 KEFLIRMPSDCPG-ILITQHMPEGFTRSFAQRLDNLCKISVKEAAGNERVLPGHAYIAPG 178 L +P G ++I QH+ F AQ L + V A + G +A Sbjct: 174 ATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAAS 233 Query: 179 HSHLKLVRSGA--NYMTQLDQGPPVNRHRPSVDVLFNSAAACAGKNAVGVILTGMGKDGA 236 + HL +V+ G Y+ +G P + +RPSVDV F S G++LTGMG+DGA Sbjct: 234 NRHL-VVKEGGRLGYV----EGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGA 288 Query: 237 LGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGGAHEVLALQAMPGRVLSYL 289 G+ +R++GA+ AQD+ SC V+GMP+ A+A+ A EVL + M R+ + + Sbjct: 289 AGLKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQAII 341 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 91.3 bits (225), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 65/190 (34%), Positives = 90/190 (47%), Gaps = 6/190 (3%) Query: 92 PLPQMR-NPLLSSEKLIIIGASTGGTEAIKEFLIRMPSDCP-GILITQHMPEGFTRSFAQ 149 PLP++ P+ + I+IGAS GG EA+ E L + ++ H+P AQ Sbjct: 5 PLPELAARPI----EAIVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQ 60 Query: 150 RLDNLCKISVKEAAGNERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVD 209 + C+ V EA E G + AP HL + RS + + VN RP++D Sbjct: 61 IFADRCRQPVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAID 120 Query: 210 VLFNSAAACAGKNAVGVILTGMGKDGALGMLEMRNAGAYNFAQDEASCVVFGMPREAIAV 269 VLF SAA G +G++LTG DGA GM + AG Q V MP +A+A+ Sbjct: 121 VLFESAADVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAM 180 Query: 270 GGAHEVLALQ 279 G VL LQ Sbjct: 181 GNIDYVLTLQ 190 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 979,082 Number of extensions: 41627 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 5 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)