BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0078.p (134 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311346|ref|YP_003775438.1| flagellar biosynthesis/type III... 142 1e-35 >gi|300311346|ref|YP_003775438.1| flagellar biosynthesis/type III secretory pathway chaperone protein [Herbaspirillum seropedicae SmR1] Length = 152 Score = 142 bits (357), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 72/135 (53%), Positives = 102/135 (75%), Gaps = 3/135 (2%) Query: 1 VAALQQEQDLLSGNGDADKIPEIINDKANIVANMATLADQRHKALAALGFTAGENGMQSW 60 V L+QEQ LL+ + + + E+I++KA IVA+MATLA RH L LG++ E GMQ W Sbjct: 18 VEKLRQEQALLA-DATPEGLTEVISEKATIVADMATLAGNRHALLGQLGYSEDEAGMQKW 76 Query: 61 IEQHGSAEDKQVWEELFALAQ-SRELNRLNGLLIGKQMAINQNAMNILQGKSN-GSFYGP 118 + +HGS D++ W LF LAQ ++ELNR+NGLLIGK++++NQ+A+ ILQGK+ G+FYGP Sbjct: 77 MNEHGSETDREHWAALFELAQDAKELNRVNGLLIGKKISLNQSALTILQGKTGAGNFYGP 136 Query: 119 DGQSSLRSSGRPLGI 133 DGQ+S+ +SGR LG+ Sbjct: 137 DGQNSISASGRRLGV 151 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.130 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 444,321 Number of extensions: 16473 Number of successful extensions: 37 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 35 Number of HSP's successfully gapped: 1 Length of query: 134 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 58 Effective length of database: 1,260,010 Effective search space: 73080580 Effective search space used: 73080580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 125 (52.8 bits)