BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0077.p (60 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311347|ref|YP_003775439.1| anti-sigma-28 factor protein [H... 84 2e-18 >gi|300311347|ref|YP_003775439.1| anti-sigma-28 factor protein [Herbaspirillum seropedicae SmR1] Length = 121 Score = 84.3 bits (207), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 40/59 (67%), Positives = 49/59 (83%) Query: 1 LSSQYQSLGVKAAATGSTFDAKKVAEIKAAIANGTFQVDPEKVANGLFDTVKDLIGSRK 59 LS+ YQSL K +++ +FDAKKVAEIKAAIANGTFQ+D +KVANGL DTVK LI +R+ Sbjct: 62 LSATYQSLETKVSSSSGSFDAKKVAEIKAAIANGTFQIDADKVANGLIDTVKTLINARR 120 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.310 0.126 0.329 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 136,656 Number of extensions: 2936 Number of successful extensions: 9 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 9 Number of HSP's successfully gapped: 1 Length of query: 60 Length of database: 1,619,870 Length adjustment: 33 Effective length of query: 27 Effective length of database: 1,463,615 Effective search space: 39517605 Effective search space used: 39517605 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 123 (52.0 bits)