BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0075.p (140 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311349|ref|YP_003775441.1| Flagellar basal-body rod protei... 207 2e-55 >gi|300311349|ref|YP_003775441.1| Flagellar basal-body rod protein FlgB [Herbaspirillum seropedicae SmR1] Length = 142 Score = 207 bits (527), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 97/142 (68%), Positives = 117/142 (82%), Gaps = 2/142 (1%) Query: 1 MAAKLDDYLRFNETALNLRAQRQQLLASNIANADTPNYKARDIDFAKTLQGAMAGKSSTV 60 M +KLDD+ RFNE A+NLRAQRQQ+LASNIANADTPNYKARD++FA L+ A+ K++ + Sbjct: 1 MVSKLDDFFRFNEMAMNLRAQRQQVLASNIANADTPNYKARDVNFADALKNALDNKATAM 60 Query: 61 PPVELARTSSAHISGKA--TGDGGIGGSPLLYRKETQSSVDGNTVDMDVERNQFTDNAVR 118 PP+ELART++ H+ G A G G IGGSPLLYR TQ SVDGNTVD+DVERN+F DN +R Sbjct: 61 PPLELARTAATHVPGTAPENGPGSIGGSPLLYRTVTQGSVDGNTVDLDVERNEFVDNGLR 120 Query: 119 YEAGITAVNAQIKGLLAAIQPG 140 YEAG+TAVN QIK +L AIQPG Sbjct: 121 YEAGVTAVNGQIKAMLNAIQPG 142 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.128 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 380,945 Number of extensions: 14003 Number of successful extensions: 42 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 41 Number of HSP's successfully gapped: 1 Length of query: 140 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 64 Effective length of database: 1,260,010 Effective search space: 80640640 Effective search space used: 80640640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 126 (53.1 bits)