BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0072.p (425 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protei... 548 e-157 gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbasp... 541 e-155 gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protei... 81 2e-16 >gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protein FlgE [Herbaspirillum seropedicae SmR1] Length = 432 Score = 548 bits (1412), Expect = e-157, Method: Compositional matrix adjust. Identities = 296/434 (68%), Positives = 336/434 (77%), Gaps = 11/434 (2%) Query: 1 MSFQQGLSGLAGASKSLDVIGNNVANASTVGFKSAQAQFADMYANSMNRSGNSPVGIGVA 60 M FQQGLSGL GA+KSLDVIGNNVANASTVGFK +QAQFADMYANSMNRSGNSPVGIGV+ Sbjct: 1 MGFQQGLSGLNGAAKSLDVIGNNVANASTVGFKQSQAQFADMYANSMNRSGNSPVGIGVS 60 Query: 61 VANVQQAFTQGNVTTTNNPLDIAINGDGFFRMQGSLTDQSPMYARNGQFQLDKSGYIINP 120 V V Q FTQGN++++NNPLDIAINGDGFF++ SLT++SPMY RNGQFQLDKSGYI+NP Sbjct: 61 VPAVAQQFTQGNISSSNNPLDIAINGDGFFQLSASLTNKSPMYGRNGQFQLDKSGYIVNP 120 Query: 121 SEKGAYLTGWLGTVVGGDPVPLQIDTSNIPATATTLINTKLNLDSRLTSIDPAVTPFSPT 180 S GAYL GW GGDPVPLQIDTS+IPAT TT I+TK+NLDSRL+ P TPF+ Sbjct: 121 SMNGAYLMGWAAGAQGGDPVPLQIDTSSIPATPTTTISTKVNLDSRLSV--PTTTPFNAA 178 Query: 181 NAASYNSTTGVTMYDSLGNSYNVQTYYVKNKATSTATTTDWDVYATINNTTY---PVAGA 237 + SYNS+TGV +YDSLGN Y++QTYYVK T T TT W VYA I+NTT Sbjct: 179 DPTSYNSSTGVDLYDSLGNPYSMQTYYVKAAPTGTPPTTTWTVYAKIDNTTLNNGATPAV 238 Query: 238 PVKLDTLTFNSSGVLTA--GA----TGSAFTAAILNGPAFVQPGATFPANVALDYTGTTQ 291 PV L T+TF+S+G +T GA T + T + + G F A + + Y+G+TQ Sbjct: 239 PVALGTMTFDSTGTMTQINGAAISPTNNTLTISTAQLATVAKAGGVFAAPINISYSGSTQ 298 Query: 292 TGQAFTPVAQDQNGKAPGVLSSFSIGKDGSIVGSYSNNDTKVLGQVLLVNFANPNGLSPL 351 TG AF +AQ QNG PG LSSFS+ KDGSI+GSYSN TK LG V+LVNFANPNGL PL Sbjct: 299 TGSAFVNLAQVQNGMPPGSLSSFSVDKDGSIIGSYSNQQTKNLGTVVLVNFANPNGLQPL 358 Query: 352 GNNLYTATADAGQPLVGKPTTGQFGVLQARAVEDSNVDLTTELVNMIVAQRVYQANSQTI 411 GNNLY A++ AG PLVGKPTTG FG LQARA EDSNVDLT ELVNMIVAQRVYQANSQTI Sbjct: 359 GNNLYQASSAAGSPLVGKPTTGTFGTLQARAKEDSNVDLTAELVNMIVAQRVYQANSQTI 418 Query: 412 KVQDTVLQTLVSLR 425 KVQDTVLQTLVSLR Sbjct: 419 KVQDTVLQTLVSLR 432 >gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbaspirillum seropedicae SmR1] Length = 420 Score = 541 bits (1395), Expect = e-155, Method: Compositional matrix adjust. Identities = 291/428 (67%), Positives = 331/428 (77%), Gaps = 11/428 (2%) Query: 1 MSFQQGLSGLAGASKSLDVIGNNVANASTVGFKSAQAQFADMYANSMNRSGNSPVGIGVA 60 MSFQQGLSGL GASK LDVIGNNVANASTVGFK Q QFAD YANSMNRSGNSPVGIGV Sbjct: 1 MSFQQGLSGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSPVGIGVT 60 Query: 61 VANVQQAFTQGNVTTTNNPLDIAINGDGFFRMQGSLTDQSPMYARNGQFQLDKSGYIINP 120 VANV QAFTQGN+ TTNN LD+AINGDGFFRM SLTDQSP Y RNGQFQL+K GY+INP Sbjct: 61 VANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGYLINP 120 Query: 121 SEKGAYLTGWLGTVVGGDPVPLQIDTSNIPATATTLINTKLNLDSRLTSIDPAVTPFSPT 180 S GAYLTG+ GGDPVPL+IDT+ +PATATT ++TK+NLDSR + P V+PF P Sbjct: 121 SMNGAYLTGYPAGTNGGDPVPLKIDTATLPATATTRVSTKVNLDSRKSV--PTVSPFDPA 178 Query: 181 NAASYNSTTGVTMYDSLGNSYNVQTYYVKNKATSTATTTDWDVYATINNTTY---PVAGA 237 N SY+++TG T+YDSLGN Y++QT+YVK AT T +T W VYAT++ T P A Sbjct: 179 NPNSYSNSTGATVYDSLGNPYSLQTFYVKGAATGTPPSTTWSVYATLDGKTIINAPPATG 238 Query: 238 PVKLDTLTFNSSGVLTAGATGSAFTAAILNGPAFVQPGATFPANVALDYTGTTQTGQAFT 297 P L TL F+++G LTA T LN + GATFP +AL Y G+TQTG AF Sbjct: 239 PQLLGTLRFDATGALTAS------TGMSLNLSTLAKGGATFPGPIALGYAGSTQTGSAFA 292 Query: 298 PVAQDQNGKAPGVLSSFSIGKDGSIVGSYSNNDTKVLGQVLLVNFANPNGLSPLGNNLYT 357 +AQ Q+G PG LSSFSIGKDGSI+G+YSN T+V+GQ++LVNFANP+GL P+GNNLY Sbjct: 293 NLAQSQDGMPPGTLSSFSIGKDGSIIGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQ 352 Query: 358 ATADAGQPLVGKPTTGQFGVLQARAVEDSNVDLTTELVNMIVAQRVYQANSQTIKVQDTV 417 A+ AG LVG PTTG FG LQ RAVE SNVDLT ELVNMIVAQRVYQANSQTIKVQDTV Sbjct: 353 ASEAAGSALVGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTV 412 Query: 418 LQTLVSLR 425 LQTLVSLR Sbjct: 413 LQTLVSLR 420 >gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protein FlgG [Herbaspirillum seropedicae SmR1] Length = 260 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 48/125 (38%), Positives = 67/125 (53%), Gaps = 13/125 (10%) Query: 8 SGLAGASKSLDVIGNNVANASTVGFKSAQAQFADMYANSMNRSGNSP-----------VG 56 +GL LDVI NN+AN ST GFK ++A F D+ ++ + G +G Sbjct: 10 TGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQTQLPSGLQIG 69 Query: 57 IGVAVANVQQAFTQGNVTTTNNPLDIAINGDGFFRMQGSLTDQSPMYARNGQFQLDKSGY 116 GV ++ FTQGNV TNN D+AI G+GFF++ L D + Y R+G FQ D G Sbjct: 70 TGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQI--LLPDGTTAYTRDGAFQTDSQGQ 127 Query: 117 IINPS 121 ++ S Sbjct: 128 LVTSS 132 Score = 71.6 bits (174), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 46/115 (40%), Positives = 62/115 (53%), Gaps = 3/115 (2%) Query: 312 SSFSIGKDG--SIVGSYSNNDTKVLGQVLLVNFANPNGLSPLGNNLYTATADAGQPLVGK 369 + ++G+DG SI+ + S T++ G + + F NP GL G NLY TA +G P Sbjct: 147 TKLTVGRDGVVSIMQAGSTATTQI-GTIQVATFINPAGLESRGENLYMETAASGAPNPNN 205 Query: 370 PTTGQFGVLQARAVEDSNVDLTTELVNMIVAQRVYQANSQTIKVQDTVLQTLVSL 424 P T G L VE SNV++ ELVNMI QR Y+ NS+ I D +L L L Sbjct: 206 PGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAYEINSKAITTSDQMLAKLSQL 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.128 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,204,726 Number of extensions: 49158 Number of successful extensions: 121 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 115 Number of HSP's successfully gapped: 5 Length of query: 425 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 337 Effective length of database: 1,203,190 Effective search space: 405475030 Effective search space used: 405475030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)