BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0070.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protei... 473 e-135 gi|300311353|ref|YP_003775445.1| flagellar basal body rod protei... 92 6e-20 gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbasp... 78 8e-16 gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protei... 77 2e-15 >gi|300311354|ref|YP_003775446.1| flagellar basal-body rod protein FlgG [Herbaspirillum seropedicae SmR1] Length = 260 Score = 473 bits (1216), Expect = e-135, Method: Compositional matrix adjust. Identities = 228/260 (87%), Positives = 246/260 (94%) Query: 1 MIRSLWIAKTGLDAQQTQLDVISNNLANTSTNGFKRSRAVFEDLMYQTLRQPGAQSSQQT 60 MIRSLWI+KTGLDAQQTQLDVISNNLAN STNGFKRSRAVFEDL+YQT+RQPGAQSSQQT Sbjct: 1 MIRSLWISKTGLDAQQTQLDVISNNLANVSTNGFKRSRAVFEDLLYQTVRQPGAQSSQQT 60 Query: 61 QLPSGLQIGTGVRPVATERIHTQGNTNQTSNDKDVAIQGAGFFQVLMPDGTTQYTRDGSF 120 QLPSGLQIGTGVRPVATERI TQGN NQT+NDKD+AIQG GFFQ+L+PDGTT YTRDG+F Sbjct: 61 QLPSGLQIGTGVRPVATERIFTQGNVNQTNNDKDLAIQGNGFFQILLPDGTTAYTRDGAF 120 Query: 121 QSDAQGQLVTSSGYVVQPPITMPANMTKLTIGRDGTVSITQAGSTATTQIGTLQIATFIN 180 Q+D+QGQLVTSSGY +QPPIT+P N TKLT+GRDG VSI QAGSTATTQIGT+Q+ATFIN Sbjct: 121 QTDSQGQLVTSSGYTLQPPITVPLNTTKLTVGRDGVVSIMQAGSTATTQIGTIQVATFIN 180 Query: 181 PAGLESKGENLYAETAASGTPAPNTPGTNGAGALWQGYVETSNVNVVEEMVNMIQTQRAY 240 PAGLES+GENLY ETAASG P PN PGTNGAGALWQGYVETSNVNVVEE+VNMIQTQRAY Sbjct: 181 PAGLESRGENLYMETAASGAPNPNNPGTNGAGALWQGYVETSNVNVVEELVNMIQTQRAY 240 Query: 241 EMNSKAITTSDQMLAKLSQL 260 E+NSKAITTSDQMLAKLSQL Sbjct: 241 EINSKAITTSDQMLAKLSQL 260 >gi|300311353|ref|YP_003775445.1| flagellar basal body rod protein FlgF [Herbaspirillum seropedicae SmR1] Length = 245 Score = 91.7 bits (226), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 86/267 (32%), Positives = 116/267 (43%), Gaps = 33/267 (12%) Query: 1 MIRSLWIAKTGLDAQQTQLDVISNNLANTSTNGFKRSRAVFEDLMYQTLRQPGAQSSQQT 60 M R ++ A +G Q SNNLAN +T GF+ F Sbjct: 1 MDRLVYTAMSGAKHTLEQQATASNNLANATTTGFRAQLNTFR---------------AAP 45 Query: 61 QLPSGLQIGTGVRPVATERIHTQGNTNQTSNDKDVAIQGAGFFQVLMPDGTTQYTRDGSF 120 + GL T V +QG T DVAIQG G+ V DG YTR+GSF Sbjct: 46 VVGDGLPTRTFVVDSTAGNDFSQGPIQDTGRALDVAIQGRGWIAVQTADGGEAYTRNGSF 105 Query: 121 QSDAQGQLVTSSGYVVQ---PPITMPANMTKLTIGRDGTVSI--TQAGSTATTQIGTLQI 175 + + G L T SG V PIT+P + T + I DGTVS T A +G +++ Sbjct: 106 KINENGILQTQSGQTVLGDGGPITIPPDAT-VAIAADGTVSAIPTNGIPNAVNVLGRIKL 164 Query: 176 ATFINP--AGLESKGENLYAETAASGTPAPNTPGTNGAGALWQGYVETSNVNVVEEMVNM 233 +NP ++ + L+ + P N L G +E SNVNVVE MVNM Sbjct: 165 ---VNPDEKNMKRGDDGLFRTITGAAQPDANV-------RLASGALEGSNVNVVEAMVNM 214 Query: 234 IQTQRAYEMNSKAITTSDQMLAKLSQL 260 I R +EMN K I T++ K + + Sbjct: 215 INLSRQFEMNMKMIQTAESDATKATSI 241 >gi|300312833|ref|YP_003776925.1| flagellar hook protein [Herbaspirillum seropedicae SmR1] Length = 420 Score = 77.8 bits (190), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 45/118 (38%), Positives = 65/118 (55%), Gaps = 1/118 (0%) Query: 143 PANMTKLTIGRDGTVSITQAGSTATTQIGTLQIATFINPAGLESKGENLYAETAASGTPA 202 P ++ +IG+DG++ I + T +G + + F NP GL+ G NLY + A+G+ Sbjct: 303 PGTLSSFSIGKDGSI-IGAYSNQQTRVMGQIVLVNFANPHGLQPIGNNLYQASEAAGSAL 361 Query: 203 PNTPGTNGAGALWQGYVETSNVNVVEEMVNMIQTQRAYEMNSKAITTSDQMLAKLSQL 260 TP T GAL VE SNV++ E+VNMI QR Y+ NS+ I D +L L L Sbjct: 362 VGTPTTGAFGALQGRAVEQSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSL 419 Score = 69.7 bits (169), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 44/125 (35%), Positives = 62/125 (49%), Gaps = 13/125 (10%) Query: 10 TGLDAQQTQLDVISNNLANTSTNGFKRSRAVFEDLMYQTLRQPGAQSSQQTQLPSGLQIG 69 +GL LDVI NN+AN ST GFK+ + F D ++ + G +G Sbjct: 8 SGLHGASKHLDVIGNNVANASTVGFKQGQVQFADAYANSMNRSGNSP-----------VG 56 Query: 70 TGVRPVATERIHTQGNTNQTSNDKDVAIQGAGFFQVL--MPDGTTQYTRDGSFQSDAQGQ 127 GV + TQGN T+N DVAI G GFF++ + D + +Y R+G FQ + G Sbjct: 57 IGVTVANVSQAFTQGNIATTNNALDVAINGDGFFRMAASLTDQSPRYGRNGQFQLNKDGY 116 Query: 128 LVTSS 132 L+ S Sbjct: 117 LINPS 121 >gi|300311352|ref|YP_003775444.1| flagellar basal-body rod protein FlgE [Herbaspirillum seropedicae SmR1] Length = 432 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 48/131 (36%), Positives = 69/131 (52%), Gaps = 20/131 (15%) Query: 10 TGLDAQQTQLDVISNNLANTSTNGFKRSRAVFEDLMYQTLRQPGAQSSQQTQLPSGLQIG 69 +GL+ LDVI NN+AN ST GFK+S+A F D+ ++ + G +G Sbjct: 8 SGLNGAAKSLDVIGNNVANASTVGFKQSQAQFADMYANSMNRSGNS-----------PVG 56 Query: 70 TGVRPVATERIHTQGNTNQTSNDKDVAIQGAGFFQV--LMPDGTTQYTRDGSFQSDAQGQ 127 GV A + TQGN + ++N D+AI G GFFQ+ + + + Y R+G FQ D Sbjct: 57 IGVSVPAVAQQFTQGNISSSNNPLDIAINGDGFFQLSASLTNKSPMYGRNGQFQLD---- 112 Query: 128 LVTSSGYVVQP 138 SGY+V P Sbjct: 113 ---KSGYIVNP 120 Score = 75.5 bits (184), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 42/118 (35%), Positives = 65/118 (55%), Gaps = 1/118 (0%) Query: 143 PANMTKLTIGRDGTVSITQAGSTATTQIGTLQIATFINPAGLESKGENLYAETAASGTPA 202 P +++ ++ +DG++ I + T +GT+ + F NP GL+ G NLY ++A+G+P Sbjct: 315 PGSLSSFSVDKDGSI-IGSYSNQQTKNLGTVVLVNFANPNGLQPLGNNLYQASSAAGSPL 373 Query: 203 PNTPGTNGAGALWQGYVETSNVNVVEEMVNMIQTQRAYEMNSKAITTSDQMLAKLSQL 260 P T G L E SNV++ E+VNMI QR Y+ NS+ I D +L L L Sbjct: 374 VGKPTTGTFGTLQARAKEDSNVDLTAELVNMIVAQRVYQANSQTIKVQDTVLQTLVSL 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.125 0.344 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 756,822 Number of extensions: 31005 Number of successful extensions: 84 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 79 Number of HSP's successfully gapped: 6 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 129 (54.3 bits)