BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0064.p
         (627 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311358|ref|YP_003775450.1| flagellar hook-associated FlgK ...   517   e-147

>gi|300311358|ref|YP_003775450.1| flagellar hook-associated FlgK
           protein [Herbaspirillum seropedicae SmR1]
          Length = 778

 Score =  517 bits (1331), Expect = e-147,   Method: Compositional matrix adjust.
 Identities = 336/652 (51%), Positives = 427/652 (65%), Gaps = 34/652 (5%)

Query: 1   VLSAGSTLAARFQSLNDQMAQSRTSVNAQITSSIGSINSLATQIQELNQAIIKAQGSGGG 60
           VLS  STL AR  S+NDQ++ S  SVN+QITS++ SINS A QI +LNQAI+KA G+GGG
Sbjct: 134 VLSQASTLVARISSINDQLSSSSASVNSQITSTVTSINSFAQQISQLNQAIVKAIGTGGG 193

Query: 61  AAPNDLLDQRDQAIADLNKIVKVTTLPQNNGSMSVFIGNGQPLVLGDQVSQLVATPADDD 120
             PNDLLDQRDQ I+ LNK+VK+TT+PQ+ GS+SVFIG GQ LV GD V+Q+V T +  D
Sbjct: 194 QPPNDLLDQRDQVISQLNKLVKITTVPQDTGSVSVFIGTGQSLVAGDNVTQMVVTNSPTD 253

Query: 121 PTRLQVSIKTSSNATILLPDGAF-TGGSLGGILQYRTETLDTAQNTLGRVAIAMASAFNA 179
            +RLQ+        T  +PD  F  GGSLGG+L+YR+ETLD  QN LGR+AIAM +AFN 
Sbjct: 254 VSRLQIGQAIPGGGTSTIPDSFFYDGGSLGGLLKYRSETLDPTQNALGRIAIAMGTAFNQ 313

Query: 180 QHKLGQDQNGALGTDFFNVPVPSVVANIKNHVALPVATATATISNPSALTTSDYRLDYDG 239
           Q KLG DQNG  GT  FNV  P++   I           T TI++PSALTTSDY L YDG
Sbjct: 314 QQKLGLDQNGNQGTALFNVSSPNL---IGYPGNTGTTNLTTTITDPSALTTSDYTLSYDG 370

Query: 240 FNYSVTRLSDNTKTTSA--TMPVTIDGVDF--GSAG-------MVAKDSFLIKPTIDGAN 288
            NY+ TRLSDNTKT  A    PVT+DGV +  G+A        M A +++ I+PT +GA 
Sbjct: 371 TNYTFTRLSDNTKTIKAPGAFPVTLDGVTYSNGAAAPGPFAPTMAAGNTYKIQPTANGAA 430

Query: 289 GISVAITNTQKIAAGTPIVTSSNAVTNMGL-VTSGNTGTGVVTSATVDKAAFAPNSTQTY 347
           G S+A+ NTQ +A   PI TS NA  N+   + + NTG  ++++ ++D + +   S+ ++
Sbjct: 431 GFSLALNNTQLLATAAPIATSINATNNVNASMPASNTGNAIISNTSLDSSQYQQGSSVSF 490

Query: 348 TYSSGTNT---LALSPN-----PVGNVMVTTTAGVTTPYTPGTAIPYSAGATITADGVSF 399
           T +   +T   + LS       PV  V V    G T   T  T   Y +G TIT+ GV++
Sbjct: 491 TAALDASTPPKMQLSAAWTGAAPVPGVTVNYADGTTASVTGATPFDYKSGMTITSGGVTY 550

Query: 400 VLSGTPANNDKFVFSPTPANTGTATISSGTINSSFTATTLPAGSKLSFTFDSTTGTLATS 459
            L+G+PA  DKF F+P  AN GTATIS+G++  ++  TT P     + T++ST    A  
Sbjct: 551 ALTGSPAAGDKFNFAPVSANKGTATISTGSVTPAYLTTTTPLTKPTTLTYNST----AAP 606

Query: 460 PALGTAPM-----GTVTVKDAGGNPVLVTPPNLPFGPGYSYTTSTGMSFSITGQPANGDQ 514
           P    +P      GT+T KD     +      L +  G SY  S G+ F+I+GQP NGDQ
Sbjct: 607 PVFNISPAVPAGGGTITHKDGTTTTIAGGATTLSYTAGDSYEIS-GVQFAISGQPGNGDQ 665

Query: 515 FYVSPNTGGVTDNRNALALNALQTANTIGNTSFQGSYSQLVSLIGNKTNEITVTNKAENA 574
           F ++ NT   +DNRNALA+  LQTANTI  TSFQGSYSQLV+ IGNKTNEI VTN AE A
Sbjct: 666 FTIAANTNAASDNRNALAMGKLQTANTINGTSFQGSYSQLVATIGNKTNEINVTNTAEKA 725

Query: 575 RLTAIQQEQQSESGVNQDEELANMIKNQTAYQAAAKIIQAASDMLNVLFTLG 626
           RLTAIQ +QQSESGVNQDEELA+MI+NQ  YQAAAKIIQAASDM+NVL +LG
Sbjct: 726 RLTAIQNQQQSESGVNQDEELAHMIQNQQQYQAAAKIIQAASDMINVLLSLG 777


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.309    0.125    0.340 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,721,115
Number of extensions: 70638
Number of successful extensions: 187
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 180
Number of HSP's successfully gapped: 1
Length of query: 627
Length of database: 1,619,870
Length adjustment: 91
Effective length of query: 536
Effective length of database: 1,188,985
Effective search space: 637295960
Effective search space used: 637295960
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 133 (55.8 bits)