BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0058.p (162 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311366|ref|YP_003775458.1| flagellar biogenesis protein Fl... 117 5e-28 >gi|300311366|ref|YP_003775458.1| flagellar biogenesis protein FliO [Herbaspirillum seropedicae SmR1] Length = 206 Score = 117 bits (293), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 71/95 (74%), Positives = 79/95 (83%) Query: 41 LPSATGSVFTMLLGLTAVLAIMAGIAWLFKRTGLVQRAQGNAVAKIIGGVSVGTRERVMV 100 LPS+ GSVFTMLLGLT VLA++A +AWLFKR GL Q GNA AK++GGVSVGTRERVMV Sbjct: 77 LPSSGGSVFTMLLGLTVVLAVLAAVAWLFKRFGLNQGLAGNAAAKVVGGVSVGTRERVMV 136 Query: 101 IEVADQWIVIGVAPGRVNTLATMPRQEHVAAPASA 135 IEV +QWIV+GVAPGRVN LATMPRQ AA A A Sbjct: 137 IEVGEQWIVVGVAPGRVNALATMPRQTLAAAQAGA 171 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.132 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 352,504 Number of extensions: 10970 Number of successful extensions: 65 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 65 Number of HSP's successfully gapped: 1 Length of query: 162 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 84 Effective length of database: 1,250,540 Effective search space: 105045360 Effective search space used: 105045360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 127 (53.5 bits)